Summary: Glomerular cells have been grown in a reproducible manner from 5 normal human kidneys. A technique is described which combines mechanical disruption of renal cortex and microdissection, and provides large numbers of pure glomeruli within 30-45 minutes. Histological examination shows this technique produces intact glomeruli without cell disturbance.
During tissue culture, glomeruli attach to the flask and the intrinsic cells migrate onto the flask and divide. Variations of culture conditions have shown that glomeruli are robust without fastidious culture requirements. Intact kidney tissue can be left at 4[degrees]C for periods up to 24 hours prior to isolation of individual glomeruli without affecting subsequent cellular growth in culture. They grow in most commonly used media although the cells require 20% foetal calf serum for optimum growth. Their pH optimum is between 7.0 and 7.4 with temperature optimum of 37[degrees]C. As glomeruli must attach prior to cell growth, minimum movement is critical to promote optimum growth.
Under these optimum conditions a regular and predictable growth of cells of two distinct types, has been observed over 14 days; one of these types is probably epithelial.
(C) 1978 Royal College of Pathologists of Australasia