Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis : Pancreas

Secondary Logo

Journal Logo

Advanced Search
Original Articles

Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis

Kawakubo, Kazumichi MD, PhD; Ohnishi, Shunsuke MD, PhD; Fujita, Hirotoshi BPharm; Kuwatani, Masaki MD, PhD; Onishi, Reizo MD, PhD; Masamune, Atsushi MD, PhD; Takeda, Hiroshi MD, PhD; Sakamoto, Naoya MD, PhD

Author Information

From the *Department of Gastroenterology and Hepatology, Graduate School of Medicine, †Laboratory of Pathophysiology and Therapeutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan; and ‡Division of Gastroenterology, Graduate School of Medicine, Tohoku University, Sendai, Japan.

Received for publication February 22, 2015; accepted August 5, 2015.

Reprints: Shunsuke Ohnishi, MD, PhD, Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University, N15, W7, Kita-ku, Sapporo 060-8638, Japan (e-mail: [email protected]).

This work was supported by a Grant-in Aid for Young Scientists (B) from the Japan Society for the Promotion of Science (JSPS, 24790670) and by the Pancreas Research Foundation of Japan.

The authors declare no conflict of interest.

Pancreas 45(5):p 707-713, May/June 2016. | DOI: 10.1097/MPA.0000000000000541

Abstract

Objectives 

Mesenchymal stem cells (MSCs) are a valuable cell source in regenerative medicine and can be isolated from fetal membranes (FMs), particularly amniotic membranes. We investigated the effect of rat FM-derived MSCs (rFM-MSCs) and human amnion-derived MSCs (hAMSCs) on the inflammatory reaction in vitro and therapeutic effects in rats with acute and chronic pancreatitis.

Methods 

Effect of rFM-MSCs or hAMSC-conditioned medium was investigated in vitro. Acute pancreatitis was induced by intraductal injection of 4% taurocholate, and rFM-MSCs were transplanted intravenously. Chronic pancreatitis was induced by intravenous injection of 5 mg/kg dibutyltin dichloride, and hAMSCs were transplanted intravenously.

Results 

The inflammatory reaction of macrophages induced by lipopolysaccharide and trypsin was significantly suppressed by rFM-MSC coculture. Pancreatic acinar cell injury induced by cerulein was significantly ameliorated by hAMSC-conditioned medium. Pancreatic stellate cell activation induced by tumor necrosis factor-α was significantly decreased by hAMSC-conditioned medium. Transplantation of rFM-MSCs significantly reduced the histological score and infiltration of CD68-positive macrophages in the rat pancreas. The hAMSC transplantation significantly decreased the expression of MCP-1 and attenuated the downregulation of amylase expression in the pancreas.

Conclusions 

Transplantation of FM-MSCs and AMSCs suppressed the inflammatory reaction of acute and chronic pancreatitis in rats.

Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.

Full Text Access for Subscribers:

Not a Subscriber?

Buy
Request Permissions

You can read the full text of this article if you:

Access through Ovid