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The Research of Acellular Pancreatic Bioscaffold as a Natural 3-Dimensional Platform In Vitro

Wang, Xin, MD*†‡; Li, Yue-Guang, MD§; Du, Yue, PhD; Zhu, Ji-Ye, MD, PhD¶#; Li, Zhao, MD, PhD¶#

doi: 10.1097/MPA.0000000000001123
Original Article: PDF Only

Objective The aim of the study was to investigate the biochemical and functional properties of a rat acellular pancreatic bioscaffolds (APBs).

Methods Fresh pancreata from 10 rats were soaked and perfused through portal veins using Easy-Load Digital Drive peristaltic pumps. The histological structure, extracellular matrix composition, and the DNA content of the APBs were evaluated. Biocompatibility studies had also been performed. The proliferation and differentiation of AR42J pancreatic acinar cells were assessed.

Results The pancreatic tissue became translucent after decellularization. There were no visible vascular endothelial cells, cellular components, or cracked cellular debris. The extracellular matrix components were not decreased after decellularization (P > 0.05); however, the DNA content was decreased significantly (P < 0.05). The subcutaneous implantation sites showed low immunological response and low cytotoxicity around the APB. The proliferation rate was higher and the apoptosis rate was lower when AR42J cells were cultured on APB (P < 0.05). The gene expression and the protein expression were higher for the APB group (P < 0.001).

Conclusions Our findings support the biological utility of whole pancreas APBs as biomaterial scaffolds, which provides an improved approach for regenerative medicine.

From the *Key Laboratory of Hormones and Development (Ministry of Health), Key Laboratory of Metabolic Diseases, Tianjin Metabolic Diseases Hospital. Institute of Endocrinology, Tianjin Medical University;

Department of General Surgery, The Fourth Center Hospital

Division of Bioengineering, Department of Regenerative Medicine, The Fourth Center Hospital;

§Department of Hepatobiliary Surgery, Nankai Hospital;

Department of Public Health, Tianjin Medical University, Tianjin;

Department of Hepatobiliary Surgery, Peking University People's Hospital, Beijing; and

#Beijing Key Laboratory of Liver Cirrhosis and HCC Basic Research, Beijing, China.

Received for publication November 2, 2017; accepted June 12, 2018.

Address correspondence to: Zhao Li, MD, PhD, Department of Hepatobiliary Surgery, Peking University People's Hospital, No. 11 Xizhimen South St, Xicheng District, Beijing 100044, China (e-mail: wx007146@163.com).

The authors declare no conflict of interest.

The study was supported by National Science Foundation of China (Number 81502509) and Science Foundation of Tianjin (Number 2014KY04).

X.W. and Y.G.L. contributed equally to this work;

X.W., J.Y.Z., and Z.L. designed the research; X.W. and Y.D. performed the research; Y.G.L. and Y.D. contributed new reagents/analytic tools; X.W. and Z.L. analyzed the data and wrote the article.

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