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Galectin-1 Secreted by Activated Stellate Cells in Pancreatic Ductal Adenocarcinoma Stroma Promotes Proliferation and Invasion of Pancreatic Cancer Cells: An In Vitro Study on the Microenvironment of Pancreatic Ductal Adenocarcinoma

Xue, Xiaofeng MD*; Lu, Zipeng MD; Tang, Dong MD*; Yao, Jie MD, PhD; An, Yong MD*; Wu, Junli MD, PhD; Li, Qiang MD; Gao, Wentao MD, PhD; Xu, Zekuan MD, PhD; Qian, Zhuyin MD, PhD; Dai, Cuncai MD, PhD; Wei, Jishu MD, PhD; Miao, Yi MD, PhD*†; Jiang, Kuirong MD, PhD

doi: 10.1097/MPA.0b013e318217945e
Original Articles
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Objectives: This study aimed to clarify that the activated pancreatic stellate cells (PaSCs) are the origin of the highly expressed galectin-1 in the stroma of pancreatic ductal adenocarcinoma (PDAC) tissue and to evaluate the effect of the secreted galectin-1 on proliferation and invasion ability of pancreatic cancer cell line CFPAC-1 in vitro.

Methods: Different kinds of PaSCs were isolated from the normal or cancerous pancreatic tissues and cultured. Immunohistochemistry study, quantitative polymerase chain reaction, and Western blot were carried out to check the cellular origin of galectin-1 in PDAC tissue. By using modified Boyden chambers, in vitro coculture system of PaSCs was established with the pancreatic cancer cell line CFPAC-1 and based on which we assessed the proliferation and invasion ability of CFPAC-1 with or without galectin-1 antagonists.

Results: We identified PaSCs as the primary source of the highly expressed galectin-1 in PDAC stroma. Galectin-1 secreted by PaSCs increased CFPAC-1 proliferative rate in the proliferation assay and facilitated CFPAC-1 infiltration in the invasion assay.

Conclusions: Under malignant circumstances, PaSCs express and secret galectin-1, which could further promote the proliferation and invasion of cancer cells.

From the *Laboratory of General Surgery, †Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing; and ‡Department of General Surgery, The First Affiliated Hospital of Yangzhou University, Yangzhou, PR China.

Received for publication July 30, 2010; accepted February 25, 2011.

Reprints: Yi Miao, MD, PhD; Kuirong Jiang, MD, PhD, Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Rd, Nanjing 210029, PR China (e-mail: miaoyi@njmu.edu.cn).

Xue and Lu contributed equally to this study.

This study was supported by the National Natural Science Foundation of China (no. 30972911).

© 2011 Lippincott Williams & Wilkins, Inc.