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The role of Nav1.7 in human nociceptors

insights from human induced pluripotent stem cell–derived sensory neurons of erythromelalgia patients

Meents, Jannis E.a; Bressan, Elisangelaa; Sontag, Stephanieb,c; Foerster, Aleca; Hautvast, Petraa; Rösseler, Corinnaa; Hampl, Martina,d; Schüler, Herdite; Goetzke, Romanf; Le, Thi Kim Chia; Kleggetveit, Inge Petterg; Le Cann, Kima; Kerth, Claraa; Rush, Anthony M.h; Rogers, March; Kohl, Zachariasi; Schmelz, Martinj; Wagner, Wolfgangb,f; Jørum, Elleng,k; Namer, Barbarad; Winner, Beatel; Zenke, Martinb,c; Lampert, Angelikaa,*

doi: 10.1097/j.pain.0000000000001511
Research Paper
Open
SDC

The chronic pain syndrome inherited erythromelalgia (IEM) is attributed to mutations in the voltage-gated sodium channel (NaV) 1.7. Still, recent studies targeting NaV1.7 in clinical trials have provided conflicting results. Here, we differentiated induced pluripotent stem cells from IEM patients with the NaV1.7/I848T mutation into sensory nociceptors. Action potentials in these IEM nociceptors displayed a decreased firing threshold, an enhanced upstroke, and afterhyperpolarization, all of which may explain the increased pain experienced by patients. Subsequently, we investigated the voltage dependence of the tetrodotoxin-sensitive NaV activation in these human sensory neurons using a specific prepulse voltage protocol. The IEM mutation induced a hyperpolarizing shift of NaV activation, which leads to activation of NaV1.7 at more negative potentials. Our results indicate that NaV1.7 is not active during subthreshold depolarizations, but that its activity defines the action potential threshold and contributes significantly to the action potential upstroke. Thus, our model system with induced pluripotent stem cell–derived sensory neurons provides a new rationale for NaV1.7 function and promises to be valuable as a translational tool to profile and develop more efficacious clinical analgesics.

Human sodium channel NaV1.7 in induced pluripotent stem cell–derived sensory neurons sets the action potential threshold but does not support subthreshold depolarizations.

aInstitute of Physiology, Medical Faculty, RWTH Aachen University, Aachen, Germany. Dr. Bressan is now with the German Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany

bDepartment of Cell Biology, Institute for Biomedical Engineering, Medical Faculty, RWTH Aachen University, Aachen, Germany. Dr. Sontag is now with the Taconic Biosciences GmbH, Köln, Germany

cHelmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany

dInstitute for Physiology and Pathophysiology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany

eInstitute of Human Genetics, Uniklinik RWTH Aachen, Aachen, Germany

fDivision of Stem Cell Biology and Cellular Engineering, Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany

gSection of Clinical Neurophysiology, Department of Neurology, Oslo University Hospital-Rikshospitalet, Oslo, Norway

hMetrion Biosciences, Cambridge, United Kingdom

iDepartment of Molecular Neurology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany

jDepartment of Experimental Pain Research Mannheim, Heidelberg University, Mannheim, Germany

kInstitute of Clinical Medicine, University of Oslo, Oslo, Norway

lDepartment of Stem Cell Biology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany

Corresponding author. Address: Institute of Physiology, Medical Faculty, RWTH Aachen University, Pauwelsstr 30, 52074 Aachen, Germany. Tel.: 0049 241 8088810; fax: 0049 241 8082434. E-mail address: alampert@ukaachen.de (A. Lampert).

Sponsorships or competing interests that may be relevant to content are disclosed at the end of this article.

Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Web site (www.painjournalonline.com).

J.E. Meents, E. Bressan, S. Sontag, and A. Foerster contributed equally to this work.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

Received September 13, 2018

Received in revised form January 09, 2019

Accepted January 25, 2019

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1. Introduction

About 10 years ago, patients were described with a complete loss of function of the voltage-gated sodium channel NaV1.7. These patients were unable to sense any pain, whereas other senses of the skin remained unaltered.9 Shortly before, gain-of-function mutations in NaV1.7 in patients suffering from chronic pain syndromes, such as inherited erythromelalgia (IEM), had been identified.23,45 These discoveries sparked hope for millions suffering from chronic pain: a new and promising drug target had emerged. Consequently, inhibitors of NaV1.7 were developed and some entered clinical trials. Unfortunately, to date no, NaV1.7 inhibitors have shown significant clinical efficacy, and several have been given up (https://clinicaltrials.gov). Why are specific NaV1.7 inhibitors effective in heterologous systems and rodent models, but not in clinical trials? Our understanding is limited by the fact that it is difficult to investigate native human sensory neurons.

Recent recordings of postmortem dissociated human dorsal root ganglion (DRG) neurons suggest that NaV1.7 displays fundamentally different gating and pharmacological sensitivity to NaV1.7 inhibitors in human tissue compared with rodents or heterologous models.47 It is generally assumed that subthreshold depolarizations that initiate action potentials are due to NaV1.7-mediated ramp currents.12,21 However, human DRG do not display such TTX-s ramp currents.47 Based on experiments using knockout animals, NaV1.7 seems to contribute no more than 25% of the TTX-s current in sensory neurons.31 Several studies have highlighted the differences between rodent and human nociceptors.13,20,35,37 A recent report even suggests a role of NaV1.7 in regulation of gene expression, rather than in excitability.29 Thus, NaV1.7 is a validated target for pain treatment in humans, and we need human translational models to assess gating and pharmacology of NaV1.7 in a physiological background.

The well-described IEM mutation NaV1.7 p.I848T45 (NaV1.7/I848T) induces a shift of voltage dependence of activation to hyperpolarized potentials in HEK cells.11,40,43 This facilitates opening of the channel and has been linked to a reduced firing threshold and to an increased evoked firing frequency of rodent neurons.19 Thus, in rodents, potentially painful symptoms could be explained intuitively. However, it is unclear whether these assumptions translate to human nociceptors.

In a previous study, induced pluripotent stem cell (iPS cell)-derived sensory neurons from a patient with the NaV1.7/I848T mutation failed to show a significant increase in evoked firing frequency, although the neurons displayed a reduced action potential rheobase and expressed sodium currents sensitive to a potentially selective NaV1.7 inhibitor.5 However, selectivity in native human tissue may be different.47 Because no voltage-clamp experiments were conducted, the role of NaV1.7 is unclear, also if the IEM mutation induced a leftward shift of activation as suggested from heterologous expression systems. With this study, we aim to address these questions. We differentiated iPS cells from 2 related IEM patients (carrying NaV1.7/I848T) into sensory neurons. We determined the voltage dependence of activation of the mutated NaV1.7 and how this affects action potential firing. Our results highlight the potential of this human model system for the development of new analgesic strategies.

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2. Methods

2.1. Subjects

This study was conducted with iPS cells, derived from fibroblasts of 2 female subjects of the same family (mother and daughter, age at biopsy 59 and 36 years, respectively) clinically and genetically characterized with IEM.48 The skin biopsies were obtained in Oslo, Norway, and fibroblast lines were generated at the University of Erlangen-Nürnberg following written informed consents of the patients (in Norwegian) with approval by the Institutional Review Board (Nr. 4120: “Generierung von humanen neuronalen Modellen bei neurodegenerativen Erkrankungen”), Department of Molecular Neurology, Universitätsklinikum Erlangen (Erlangen, Germany), and the local ethical committee South East Norway. Induced pluripotent stem cells derived from the 2 IEM patients are labelled IEM 1 (mother; EBiSC repository name UKAi0005-A) and IEM 2 (daughter; EBiSC repository name UKAi0006-A). HUES6 ES cells and iPS cells of healthy Caucasian non-IEM subjects (Ctrl 1, female, age 30 years, unpublished; Ctrl 2, female, age 3 years, previously referred to as iPS_233) were used as control. All experiments with human embryonic stem cells (HUES6) were performed in accordance with the German Stem Cell Act (RKI AZ.3.04.02/0085) and in the same manner as described below for iPS cells.

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2.2. Generation and maintenance of induced pluripotent stem cells

Fibroblasts of IEM and Ctrl patients were reprogrammed into iPS cells using the CytoTune-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific, Schwerte, Germany) containing Sendai virus vectors for OCT4, KLF4, SOX2, and c-MYC. One day before transduction, fibroblasts were seeded at 2 × 104 cells/cm2 onto 0.1% gelatin-coated (Sigma-Aldrich, Taufkirchen, Germany) tissue culture plates and cultured in high-glucose DMEM, supplemented with 10% fetal calf serum, 100 U/mL penicillin, 100 µg/mL streptomycin, and 2 mM L-glutamine (all Thermo Fisher Scientific). For transduction, 15 µL of vector mix were applied and cells were cultured with virus particles for 2 days. On day 5 after transduction, fibroblasts were washed with 1× phosphate-buffered saline (PBS; Thermo Fisher Scientific) and transferred onto irradiated mouse embryonic fibroblasts. After day 6, cells were cultured in KnockOut-DMEM (KO-DMEM) supplemented with 20% KnockOut serum replacement, 100 U/mL penicillin, 100 µg/mL streptomycin, 2 mM L-glutamine, 0.1 mM nonessential amino acids (NEAA), 0.1 mM β-mercaptoethanol (all Thermo Fisher Scientific), and 10 ng/mL basic fibroblast growth factor (bFGF; PeproTech, Hamburg, Germany). Colonies were picked after 3 weeks. Induced pluripotent stem cells were maintained on Matrigel (Corning, Kaiserslautern, Germany) in mTeSR1 and passaged with dispase (both Stemcell Technologies, Cologne, Germany) every 5 to 6 days.

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2.3. Sanger sequencing

Genomic DNA of iPS cells was isolated using the NucleoSpin Tissue Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer's instructions. NaV1.7 target region was PCR-amplified with primers given in Supplementary Table S1 (available at http://links.lww.com/PAIN/A749). PCR products were purified with the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer's instructions and sent for sequencing (Eurofins Genomics, Ebersberg, Germany).

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2.4. Cytogenetic analysis

Structural and numerical chromosome alterations were investigated by conventional karyotyping using GTG banding at 400 to 550 band level. Metaphase spreads were prepared using standard procedures of blocking cell division at metaphase, hypotonic treatment, and methanol/acetic acid (3:1) fixation. GTG banding included trypsin pretreatment for partial removal of DNA-associated (chromosomal) proteins and subsequent Giemsa staining. Microscopy was performed using Axioplan fluorescence microscope (Carl Zeiss, Oberkochen, Germany) and IKARUS digital imaging systems (MetaSystems, Altlußheim, Germany). On average, 20 GTG-banded metaphases were analysed per clone.

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2.5. Embryoid body assay

To assess 3 germ layer differentiation potential, iPS cells were subjected to spontaneous differentiation as described previously.33 Briefly, embryoid bodies were formed by 30 minutes of collagenase IV (Thermo Fisher Scientific) treatment, transferred to ultralow attachment plates (Corning), and grown in suspension with KO-DMEM supplemented with 20% fetal calf serum (Lonza, Basel, Switzerland), 100 µM NEAA, 100 U/mL penicillin, 100 µg/mL streptomycin, 2 mM L-glutamine, and 0.1 mM β-mercaptoethanol. Samples were harvested for RNA isolation on day 0, 7, 14, 17, and 21 of differentiation and analyzed for germ layer–specific genes by RT-qPCR.

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2.6. Reverse transcription-quantitative PCR

RNA was isolated with the NucleoSpin RNA Kit (Macherey-Nagel) according to the manufacturer's instructions. RNA was reverse transcribed into cDNA using High-Capacity cDNA Reverse Transcriptase Kit (Thermo Fisher Scientific) or SensiFAST cDNA Synthesis Kit (Bioline, Luckenwalde, Germany). Quantitative PCR was performed on a StepOnePlus Real-Time cycler with FAST SYBR Green master mix (Thermo Fisher Scientific) or on a RotorGeneQ Real-Time cycler (Qiagen, Hilden, Germany) with SensiMix SYBR No-ROX Kit (Bioline). Human-specific primers (Eurofins Genomics) are listed in Supplementary Table S2 (available at http://links.lww.com/PAIN/A749). For heat maps (Supplementary Fig. S1c, available at http://links.lww.com/PAIN/A749), expression values were normalized per gene and subjected to bidirectional hierarchical clustering using Euclidean distance and average linkage clustering.

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2.7. DNA methylation and Epi-Pluri-Score analysis

To validate successful reprogramming into pluripotent iPS cells, DNA methylation (DNAm) status of 3 CpG sites (cg22247240 in C14orf115, cg23737055 in ANKRD46, and cg13083810 in OCT4) was analyzed in stem cells as described previously.25 In brief, genomic DNA was isolated and bisulfite-converted using the EZ DNA Methylation Kit (Zymo Research, Irvine, CA). The regions of interest were PCR-amplified using the PyroMark PCR Kit and pyrosequenced with gene-specific primers on a PyroMark Q96 ID instrument. DNAm at CpG sites was analyzed with the PyroMark CpG SW 1.0 software (all Qiagen). Methylation levels of CpG sites are characterized as β values with β value 0 and β value 1 representing 0% and 100% methylation, respectively. Epigenetic Pluripotency Score (Epi-Pluri-Score) was calculated as β value (ANKRD46) − β value (C14orf115) ranging from 1 for pluripotent cells to −1 for nonpluripotent (somatic) cells. In combination with the third CpG site in the pluripotency-associated gene OCT4, Epi-Pluri-Score classified all iPS cells and the HUES6 as pluripotent (see also Supplementary Fig. S1b, available at http://links.lww.com/PAIN/A749).

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2.8. Differentiation into sensory neurons

Differentiation of stem cells was performed as described previously.7,16 Induced pluripotent stem cells or HUES6 cells were seeded as single cells in a density of 105 cells/cm2 in the presence of 10 µM Y-27632 (Abcam Biochemicals, Bristol, United Kingdom). When cells reached 80% to 90% confluence, usually 24 hours after plating, we induced neural conversion using dual-SMAD inhibition.7 LDN-193189 (100 nM; Sigma-Aldrich) and SB431542 (10 µM; Miltenyi Biotec, Bergisch Gladbach, Germany) were added to the basal culture medium between days 0 to 5. To accelerate neural crest specification and peripheral neuron formation from neural crest cells, 3 small molecules (3 µM CHIR99021, 10 µM DAPT, and 10 µM SU5402, all Tocris, Bristol, United Kingdom)7 were added between days 2 to 10. Between days 0 and 5, cells were fed with knockout DMEM/F-12 containing 15% KnockOut serum replacement, 1 mM L-glutamine, 100 µM NEAA, 100 µM β-mercaptoethanol, 100 U/mL penicillin, and 100 µg/mL streptomycin (all from Thermo Fisher Scientific). Between days 4 and 10, cells were fed with DMEM/F-12, containing 10 mL/l N2, 20 mL/l B27 minus vitamin A supplements, 100 U/mL penicillin, and 100 µg/mL streptomycin (all from Thermo Fisher Scientific). N2/B27 medium was added to basal medium at 25% between days 4 to 5, 50% between days 6 to 7, and 75% between days 8 to 10. On day 10, cells were dissociated using Accutase (Stemcell Technologies) and seeded on glass coverslips, coated with 50 µg/mL poly-L-ornithine (Sigma-Aldrich) and 5 µg/mL laminin (Thermo Fisher Scientific). From dissociation at differentiation day 10, neurons underwent a maturation period of 8 weeks in N2/B27 medium supplemented with 20 ng/mL NGF (R&D Systems, Minneapolis, MN), BDNF, GDNF (both from PeproTech), and 200 ng/mL ascorbic acid (Sigma-Aldrich). Medium was changed every 3 to 4 days.

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2.9. Immunofluorescence imaging

For immunofluorescence staining, iPS cells or differentiated neurons were seeded onto glass cover slips, coated with Matrigel or poly-L-ornithin/laminin, respectively (Sigma-Aldrich and Thermo Fisher Scientific). Cells were fixed with 4% paraformaldehyde and blocked and permeabilized with 2% bovine serum albumin and 0.1% Triton X-100 in phosphate-buffered saline (all Sigma-Aldrich). Induced pluripotent stem cells were stained with anti-OCT4 (clone sc-9081; Santa Cruz Biotechnology, Dallas, TX) and anti-TRA-1-60 (clone TRA-1-60; Merck Millipore, Darmstadt, Germany) (Supplementary Fig. S1a, available at http://links.lww.com/PAIN/A749). Induced pluripotent stem cell–derived neurons were stained with anti-peripherin (clone sc-7604; Santa Cruz Biotechnology), anti-β-III-tubulin (clone TUJ-1; R&D Systems), anti-NaV1.8 (clone ASC-016, 1:1000, see Supplementary Fig. S2, available at http://links.lww.com/PAIN/A749), and anti-TRPV1 (clone ACC-030, 1:1000; all from Alomone Labs, Jerusalem, Israel). Secondary antibodies were goat anti-rabbit IgG Alexa Fluor 594 (OCT4, NaV1.8, TRPV1), donkey anti-mouse IgM Alexa Fluor 488 (TRA-1-60), donkey anti-goat IgG Alexa Fluor 488 (peripherin), and goat anti-mouse IgG Alexa Fluor 488 and 594 (β-III-tubulin). Nuclei were counterstained with DAPI (all Thermo Fisher Scientific). Fluorescent images were acquired using an LSM 700 confocal microscope (Carl Zeiss) with 40× oil-immersion objective, 4× line averaging, 200 mHz scanning speed, and pinhole set to 1 Airy unit. Fluorescent images were obtained on maturation day M35.

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2.10. Calcium imaging

Stem cell–derived sensory neurons were loaded with Fura-2 AM (5 µM; Biotium, Scarborough, Canada), supplemented with Pluronic F-127 (0.04%; Thermo Fisher Scientific), for 30 minutes followed by 15 minutes washout at 37°C and 5% CO2. Experiments were performed at room temperature using a Till Photonics Polychrome V monochromator (excitation wavelengths 340/380 nm) and were recorded using a 12-bit CCD camera (Imago Sensicam QE; Till Photonics, Gräfeling, Germany) at 1 Hz and an exposure time of 50 ms. The fluorescence ratio was calculated for all regions of interest after background subtraction (TILLvisION 4.0.1.3 software, Till Photonics). Cells were continuously superfused through a gravity-driven perfusion system. Extracellular solution was composed of (in mM): 145 NaCl, 5 KCl, 1.25 CaCl2, 1 MgCl2, 10 HEPES, and 10 glucose (pH 7.4). For a solution containing 60 mM KCl, the extracellular solution was prepared by reducing NaCl to 90 mM. Capsaicin (1 µM), AITC (100 µM), menthol (100 µM) (all from Sigma-Aldrich), pH 6.0, and high KCl solution (60 mM) were diluted in extracellular solution and applied for 30 seconds each at an interval of 5 minutes. Calcium imaging was performed on maturation day M35.

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2.11. Electrophysiology

Whole-cell patch-clamp recordings were performed on iPS cell–derived neurons after a minimum of 8 weeks of maturation. Experiments were performed using a HEKA EPC 10USB amplifier and PatchMaster and analyzed using FitMaster v2.8 software (all HEKA electronics, Lambrecht, Germany), Igor Pro (WaveMetrics, Portland, OR), and GraphPad Prism version 5 or 6 (GraphPad Software, Inc, La Jolla, CA). Series resistance was compensated by 65% to 85%. Currents were low-pass filtered at 10 kHz and sampled at 100 kHz. Leak current was subtracted using the P/4 method. The liquid junction potential was not corrected. All experiments were performed at room temperature.

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2.12. Current-clamp recordings

The following bath solution was used (in mM): 140 NaCl, 3 KCl, 1 MgCl2, 1 CaCl2, 10 HEPES, and 20 glucose (pH 7.4; 300-310 mOsm). Intracellular solution contained (in mM): 4 NaCl, 135 K-gluconate, 3 MgCl2, 5 EGTA, 5 HEPES, 2 Na2-ATP, and 0.3 Na-GTP (pH 7.25; 290-300 mOsm). The resting membrane potential (RMP) was measured immediately after establishing the whole-cell configuration. Holding current was then adjusted to achieve a membrane voltage of −70 ± 5 mV. Action potentials were induced by slow ramp current injections (from 0 to 500 pA in 400 ms) and subsequently by 500 ms square current injections in steps of 0.5 × rheobase stimulus (up to 5.5 × rheobase stimulus). Action potentials were only considered if they exceeded 0 mV. The first action potential evoked by the square pulse protocol was used to calculate the action potential properties. The threshold was defined as the minimum of the first derivative of the action potential (=the point of inflection during the depolarization). The afterhyperpolarization is the minimum after the action potential peak. The amplitude is measured between RMP and action potential peak. To calculate the action potential half-width, the half-distance between threshold and peak is taken and the distance between this point during depolarization and repolarization is measured. The time to peak is the duration between current pulse onset and action potential peak. The maximum slope of the upstroke was calculated between threshold and peak, whereas the slope of the subthreshold depolarization was determined between RMP and threshold. Current-clamp recordings were performed between maturation days 55 and 75. For the characterization of action potential properties, data from the 2 IEM clones (IEM 1 and IEM 2) as well as data from the 2 control clones (Ctrl 1 and Ctrl 2) were pooled.

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2.13. Voltage-clamp recordings

External solution contained (in mM): 140 NaCl, 1 MgCl2, 1 CaCl2, 10 HEPES, 1 glucose, 20 TEA-Cl, 1 4-aminopyridin, and 0.1 CdCl2 (pH 7.4; 300-310 mOsm), and the internal solution contained (in mM): 140 CsF, 10 NaCl, 10 HEPES, 1 EGTA, 5 glucose, and 5 TEA-Cl (pH 7.3; 290-300 mOsm). Five hundred nM TTX (Tocris Bioscience) was diluted in external solution and applied through a gravity-driven perfusion system. For voltage clamp, 40 ms voltage pulses from −80 mV to +40 mV were applied from a holding potential of −120 mV to acquire current–voltage curves. To isolate somatic currents and avoid space clamp artefacts, a voltage prepulse protocol was used to inactivate distant sodium channels as previously described28 (see Results). The prepulse (4-6 ms, −50 to −15 mV) was followed by a repolarizing interpulse (1 ms, −120 to −70 mV), which preceded the regular testpulse (see Fig 4a) (40 ms, −80 to +40 mV in 10 mV steps). Prepulse and interpulse voltage and duration were adjusted to each cell individually to eliminate all artefacts and obtain optimal voltage-clamp conditions. Subsequently, 500 nM TTX was applied, and the identical voltage pulse protocol was repeated. Currents measured after TTX application were considered to be TTX-resistant (TTX-r). TTX-r current traces were subtracted from current traces measured before TTX application to obtain the TTX-sensitive (TTX-s) component. Conductance (G) was calculated at each voltage (V) using the equation G = I/(V − Vrev), where Vrev is the reversal potential, determined for each cell individually. Normalized conductance–voltage curves were fitted with a Boltzmann equation: G/Gmax = Gmax/(1 + exp[(V − V1/2)/k]), where Gmax is the maximum conductance, V is the membrane voltage, V1/2 is the voltage at half-maximal channel activation, and k is the slope factor.

Voltage-clamp experiments to determine the block induced by ProTx-II were performed in self-made HEK293 cell lines, stably expressing hNaV1.7 or hNaV1.6. External solution for HEK293 cells contained (in mM): 140 NaCl, 3 KCl, 1 MgCl2, 1 CaCl2, 10 HEPES, and 20 glucose (pH 7.4, 310 mOsm). The internal solution for HEK293 cells consisted of (in mM): 10 NaCl, 140 CsF, 1 EGTA, 10 HEPES, and 18 sucrose (pH 7.33, 305 mOsm). Solutions containing ProTx-II (Smartox Biotechnology, Saint Martin d'Hères, France) were prepared on the same experimental day. The toxin was diluted to a final concentration of 10 nM (HEK293) or 5 nM (iPS cells) in external solution and adding 0.1% BSA (Sigma-Aldrich). Stock solutions of ProTx-II (20 µM) dissolved in H2O were stored at −20°C. Voltage-clamp recordings were performed between maturation days 55 and 70.

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2.14. Statistical analysis

Statistical analysis was performed using GraphPad Prism version 5 or 6 (GraphPad Software, Inc). Two groups were compared using a 2-tailed t test or a Mann–Whitney test, depending on normal distribution. Comparisons between 3 or more groups were performed using a 1-way analysis of variance followed by Bonferroni multiple comparisons test. Statistical information, including the tests used, is presented in the figure legends. The exact value of n (representing number of cells) can be found in the figure legends. For voltage-clamp data, values of n are presented in Table 1. Data are presented as mean ± SEM. P values <0.05 were considered significant. The following significance values are given *P < 0.05; **P < 0.01; ***P < 0.001. No outliers were defined or eliminated.

Table 1

Table 1

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2.15. Data availability

Inherited erythromelalgia patient–derived iPS cells will be entered into the European Bank for induced pluripotent Stem Cells (www.EbiSC.org) by the names UKAi0006-A (IEM 1) and UKAi0007-A (IEM 2). The data that support the findings of this study are available from the corresponding author on request.

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3. Results

3.1. Sensory neurons are derived from erythromelalgia–specific induced pluripotent stem cells

Induced pluripotent stem cells were generated from fibroblasts of 2 consanguineous IEM patients using nonintegrating Sendai virus vectors. Patients (mother and daughter) were heterozygous for the I848T mutation in NaV1.748 and iPS cells are referred to as IEM 1 and IEM 2 for the mother and the daughter, respectively (Figs. 1A and B). Induced pluripotent stem cells were pluripotent by morphology, expression of pluripotency markers, and Epi-Pluri-Score (Supplementary Fig. S1a–c, available at http://links.lww.com/PAIN/A749). In addition, iPS cells demonstrated a normal karyotype and were heterozygous for the I848T mutation (Supplementary Fig. S1d and e, available at http://links.lww.com/PAIN/A749). HUES6 embryonal stem cells (ES cells) and iPS cells of healthy Caucasian non-IEM subjects (Ctrl 1 and Ctrl 233) were used as control.

Figure 1

Figure 1

All iPS cell clones were differentiated into sensory neurons using small molecule inhibition7,16 for 10 days, followed by maturation using neuronal growth factors for a minimum of 8 weeks (Fig. 2A). Differentiated neurons formed dense neuronal networks, large ganglion-like structures, and stained positive for specific neuronal markers, such as the peripheral nervous system type III filament protein peripherin and the class III β-tubulin TUJ-1 (Fig. 2B). Neurons also expressed the sensory neuron–specific ion channels NaV1.8 and TRPV1 (Figs. 2C and D).

Figure 2

Figure 2

To also functionally characterize iPS cell–derived and ES cell–derived neurons, expression of transient receptor potential channels and acid-sensing ion channels, which are primarily expressed in sensory neurons and are important for sensing heat, cold, and chemical stimuli, was analyzed by calcium imaging using specific agonists (Fig. 2E; and Supplementary Fig. S3, available at http://links.lww.com/PAIN/A749). The large majority of IEM 1 and IEM 2 iPS cell–derived neurons responded to pH 6 stimulation, indicating the expression of acid-sensing ion channels. A smaller cell fraction responded to menthol, AITC, or capsaicin, indicating the functional presence of TRPM8, TRPA1, and TRPV1, respectively. To test for functional expression of sensory neuron-specific NaV channels, we performed whole-cell voltage-clamp experiments using NaV-specific blockers. Recordings during application of tetrodotoxin (TTX, 500 nM) show a prominent TTX-resistant (TTX-r) current, indicating the functional expression of NaV1.8 and potentially NaV1.9 (Fig. 2F and Table 1). In addition, application of the NaV1.7-specific blocker ProTx-II (5 nM) led to a clear reduction in inward current (25 ± 7%; n = 4), indicating the functional expression of NaV1.7 (Fig. 2G). The latter was corroborated by real-time qPCR experiments (Supplementary Fig. S4, available at http://links.lww.com/PAIN/A749).

Taken together, these results demonstrate that functional sensory neurons can be generated from IEM-specific iPS cells.

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3.2. Action potential threshold is reduced for erythromelalgia nociceptors

Based on experiments in rodent sensory neurons, the NaV1.7/I848T mutation has been suggested to induce pain in patients by depolarizing the RMP of nociceptors, lowering the action potential threshold, and increasing action potential firing.19 However, a more recent study did not fully reproduce this predicted phenotype in human iPS cell–derived sensory neurons.5 To clarify the effect of the mutation in a human system, iPS cell–derived sensory neurons from IEM and control patients (in the following referred to as IEM nociceptors and controls) were used for whole-cell current-clamp electrophysiology (Fig. 3A). We found the RMP to be slightly but not significantly hyperpolarized in IEM nociceptors (Fig. 3B). The measured RMP was generally more depolarized than what had previously been found in iPS cell–derived peripheral neurons of IEM patients,5 but was comparable with the RMP of mouse peripheral sensory neurons and of iPS cell–derived human central and peripheral neurons from healthy individuals.3,32,39,42 The action potential threshold was significantly reduced in neurons from IEM patients (Fig. 3C), suggesting an increased excitability of these cells. In addition, we found single action potential characteristics to be affected by the mutation: the hyperpolarization that follows each action potential was more pronounced in IEM nociceptors (Fig. 3D), potentially explaining the faster conduction of active C-fiber nociceptors measured previously in an IEM patient with the identical mutation30 (see Discussion). In addition, IEM nociceptors showed an increase in action potential amplitude (Fig. 3E), suggesting more synchronized activity of different NaV isoforms and indicating that IEM nociceptors generate a stronger depolarization during the action potential.

Figure 3

Figure 3

These results suggest that NaV1.7 plays an important role not only in setting the action potential threshold but also during the upstroke of the action potential. Accordingly, IEM nociceptors had a reduced action potential half-width (Fig. 3F), a reduced time-to-peak of the action potential (Fig. 3G), and a steeper slope of the upstroke (Fig. 3H). However, the slope of the subthreshold depolarization did not differ between IEM and control nociceptors (Fig. 3I), indicating that NaV1.7 may not play a major role during human subthreshold depolarizations, as suggested before47 but in contrast to findings in rodents.13

Most of the changes we observed in action potential characteristics of IEM nociceptors might support higher frequency firing. We therefore compared the evoked action potential firing frequency in response to increasing current injections (Fig. 3J). Although IEM nociceptors seemed to support higher frequency firing, there was considerable variability between cells from the same or different clones, making these data unsuitable for statistical comparison. We find a slight increase in tonic action potential firing in IEM nociceptors compared with Ctrl neurons (Supplementary Fig. S5, available at http://links.lww.com/PAIN/A749).

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3.3. A prepulse voltage protocol allows for accurate measurements of NaV voltage dependence in human sensory neurons

The reduced action potential threshold in IEM nociceptors could be indicative of a shift in voltage dependence of activation in the mutated NaV1.7/I848T, as suggested by recordings in heterologous expression systems.11,40 However, it is not known whether this shift is also present in human nociceptors. So far, IEM-related changes in voltage dependence of NaV activation have not been investigated in human sensory neurons. These data are, however, crucial to determine whether the predictions based on heterologous model systems are even clinically relevant in the patient, and whether they pave the way to the correct pharmacological targets.

We investigated the voltage dependence of activation of NaV1.7 in IEM and control nociceptors using whole-cell voltage-clamp electrophysiology. Because iPS cell–derived nociceptors were grown for 8 weeks to obtain mature neurons, these cells had grown extensive neurite networks. Such neurons are notoriously difficult to use for voltage-clamp recordings due to space clamp artefacts and distortion of the current recording by unwanted activation of peripheral sodium channels. We therefore applied a prepulse voltage protocol, specifically adjusted to each cell, to inactivate and therefore eliminate unwanted peripheral sodium channel input28 (Fig. 4A). This protocol allowed for accurate recording of somatic sodium channel activation (Fig. 4B) without space clamp artefacts. Varying prepulse voltages have no effect on voltage dependence of activation of WT NaV1.7 (Supplementary Fig. S6, available at http://links.lww.com/PAIN/A749).

Figure 4

Figure 4

Apart from NaV1.7, it has been shown that human nociceptors also express NaV1.1, 1.2, 1.3, 1.6, 1.8, and 1.9.5 After measuring the total whole-cell current (Fig. 4B), we applied the NaV inhibitor TTX (500 nM) to measure the TTX-resistant (TTX-r) current component, which is mainly mediated by NaV1.8 and 1.9 (Fig. 4C). We recorded TTX-r current in all groups apart from Ctrl 1 nociceptors (Table 1) where expression of TTX-r channels seems to be decreased. During data analysis, the TTX-r component was subtracted from the total current to obtain the NaV1.7-containing TTX-sensitive (TTX-s) current component (Figs. 4D and E). Because NaV1.7 is one of the predominant TTX-s isoforms in peripheral sensory neurons, we used this TTX-s voltage dependence to measure the effect of the IEM mutation in human iPS cell–derived nociceptors. Finally, recordings done without this prepulse protocol show clear signs of inaccurate voltage clamp with space clamp artefacts (Fig. 4F).

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3.4. The erythromelalgia mutation shifts the voltage dependence of activation to more hyperpolarized potentials

We used the specific prepulse voltage protocol to measure accurately clamped TTX-s whole-cell currents in control and IEM nociceptors (Fig. 5A). The TTX-s current–voltage relationship (Fig. 5B) was used to determine the voltage dependence of activation of all TTX-s channels (Fig. 5C). If the IEM mutation does indeed induce a shift in voltage dependence of NaV1.7, this should become apparent in the TTX-s activation curve. Activation curves from the 2 healthy control patients were almost identical with a V1/2 of −17.6 mV (Ctrl 1) and −18.7 mV (Ctrl 2). By contrast, nociceptors from the 2 patients carrying the NaV1.7/I848T mutation showed a hyperpolarized activation curve, with a V1/2 of −24.4 mV (IEM 1) and −24.7 mV (IEM 2) (Table 1 and Figs. 5C and D). This 6.4 mV shift in voltage dependence of activation can explain the lowered action potential threshold seen in current-clamp recordings and the increased pain experienced by the patients. No significant differences were found in the slope of the activation curve or in TTX-s current density (Table 1 and Fig. 5E).

Figure 5

Figure 5

A similar hyperpolarized shift was also apparent in the total current before subtraction of TTX-r currents (Figs. 5F and G), indicating that NaV1.7 plays an important role in setting the voltage dependence of activation of the neuron. When we analyzed the TTX-r current component, we observed no difference in voltage dependence of activation between IEM and control nociceptors (Fig. 5H), confirming that the hyperpolarizing shift is restricted to the TTX-s current component. It also underlines the important role of NaV1.7 because the hyperpolarized shift in the total current is not compensated or masked by TTX-r channel activity. In analyzing the TTX-r current, we noticed a shallower slope of the activation curves in all measured groups compared with the total and the TTX-s currents (Table 1 and Fig. 5H). This could be due to an overlay of very hyperpolarized and very depolarized curves and might therefore reflect considerable differences in voltage dependence of NaV1.8 and 1.9.

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3.5. Erythromelalgia–induced shift of activation is abolished by application of ProTx-II

We next sought to determine whether the hyperpolarized shift of activation, seen in IEM nociceptors, is indeed due to NaV1.7 because the TTX-s currents measured above are still a combination of NaV1.7 and other NaV isoforms. The tarantula toxin ProTx-II has been described as a sodium channel–blocking peptide with ∼100-fold selectivity to NaV1.7 compared with other NaV isoforms.27,38 We tested ProTx-II in HEK293 cells, expressing either NaV1.7 or NaV1.6 (Fig. 6A), which had been described as having the second-highest affinity to the compound.38 In our hands, ProTx-II (10 nM) blocked 57 ± 6% of the NaV1.7-mediated current (Fig. 6B) with a very slow time course, not having reached a steady state after 10 minutes. ProTx-II-mediated block of NaV1.6 current, however, was not significant compared with vehicle measurements (Figs. 6A and B).

Figure 6

Figure 6

The slow time course of the NaV1.7 block made the drug unsuitable for perfusion-driven application on iPS cell‐derived nociceptors while measuring voltage dependence of activation. We therefore added ProTx-II in an even more NaV1.7-specific concentration (5 nM, Fig. 2G) to the bath solution and performed recordings on IEM 2 and Ctrl 2 nociceptors because these had shown prominent TTX-r currents. The following recordings were performed as described above, including application of TTX. This procedure allowed for measuring the TTX-s but NaV1.7-free current component (Fig. 6C), which should be identical between IEM and control nociceptors. Indeed, voltage dependence of activation was found to be indistinguishable between IEM 2 and Ctrl 2 (Figs. 6C and D and Table 1). This confirms that the observed shift in voltage-dependent gating is indeed mediated by the mutation in NaV1.7 and is independent of other NaV isoforms. Interestingly, the activation curve of IEM 2 nociceptors was only marginally different between recordings with and without ProTx-II application. By contrast, the activation curve of Ctrl 2 nociceptors was shifted significantly to hyperpolarized potentials when NaV1.7 was blocked (Figs. 6C and D). This suggests that in control nociceptors, NaV1.7 is responsible for a significant depolarization of the voltage dependence of activation.

Block of NaV1.7 also allowed us to measure the voltage dependence of all the remaining NaV channels that are expressed in these nociceptors (total current; including both TTX-s and TTX-r currents). Interestingly, the NaV1.7-free total current in Ctrl 2 nociceptors also displayed a hyperpolarized activation compared with measurements where NaV1.7 was not blocked (Fig. 6E).

Taken together, these results demonstrate for the first time in human sensory neurons the predicted left shift of NaV1.7 activation induced by the NaV1.7/I848T mutation. This shift not only affects the action potential threshold, but also particularly enhances the action potential upstroke and could thereby lead to the increased pain phenotype of IEM patients. This study establishes human iPS cell–derived sensory neurons as a suitable model system to investigate the effect of NaV channel mutations, not only by current-clamp but also by voltage-clamp electrophysiology. It should be noted, however, that although the voltage-clamp results are comparable with those obtained in heterologous expression systems, the current-clamp results in these human sensory neurons differ substantially from recordings in rodent neurons, highlighting the importance of this translational model.

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4. Discussion

Treatment of chronic pain conditions has remained unsatisfying over the past decades despite considerable advances in our molecular understanding of these diseases. A major obstacle in developing novel analgesic strategies has been the limited availability of human tissue and relevant translational assays. Here, we describe a human model system to investigate sensory neurons and their sodium channel function, which can be used to study mutation-linked pathomechanisms and to develop and validate disease-specific compounds. The human iPS cell–derived nociceptors reveal functional characteristics of adult sensory neurons. The molecular changes induced by the NaV1.7/I848T-IEM mutation in rodent tissue are partly, but importantly not completely, mirrored in this human model system. Similar iPS cell–derived nociceptors have been shown to express both TTX-s and TTX-r NaV isoforms as well as other sensory neuron–specific ion channels,5,16,46 which corresponds well with our results.

A recent study used iPS cell–derived nociceptors to investigate the effects of the NaV1.7/I848T mutation in IEM patient neurons.5 The authors mainly found an increase in spontaneous firing and a reduction of action potential rheobase. However, contrary to what has been shown in rodent neurons,19 evoked action potential firing was not significantly increased in human iPS cell–derived neurons, comparable with our results. In addition, a novel NaV1.7 inhibitor provided only short-lasting pain relief in the I848T patient.5 Thus, the study leaves a number of open questions: (1) How important is NaV1.7 in action potential generation? (2) Is the predicted hyperpolarized shift of NaV1.7/I848T activation even present in human nociceptors? (3) Are the observed changes in the I848T neurons pathophysiologically relevant? In this study, we addressed these questions by determining the voltage dependence of NaV1.7 in iPS cell–derived nociceptors for the first time and by investigating the contribution of NaV1.7 to action potential generation.

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4.1. Voltage sensitivity of activation is hyperpolarized in erythromelalgia nociceptors

The IEM-inducing NaV1.7/I848T mutation has been predicted to cause a hyperpolarizing shift in the activation of NaV1.7, leading to a reduced action potential threshold and consequently to increased pain sensation.11,19 Our results confirm the predicted hyperpolarizing shift in the activation of NaV1.7 in human nociceptors and show that this shift does indeed lead to a reduced action potential threshold. The I848T mutation was reported to induce a 10 to 14 mV left shift of activation in heterologous expression systems.11,40 We measured a 6.4 mV difference between IEM and control nociceptors. Given the fact that the IEM nociceptors investigated here carry a heterozygous mutation and therefore contain a combination of mutant NaV1.7 and unmutated channels, our results correlate very well with the previous reports.11,40 The hyperpolarized shift was not only observed in the TTX-s current component but also in the total whole-cell current before application of TTX. This shows that the left shift of NaV1.7 activation is not compensated for by other channels or mechanisms but will most likely affect excitability in these nociceptors. Indeed, we observed a reduced action potential threshold of IEM nociceptors as compared to unmutated control cells.

Contrary to what has been predicted based on experiments in rodent sensory neurons,19 we did not see a clear or consistent increase in firing frequency due to large variability. This is in line with the aforementioned study on patient iPS cell–derived nociceptors5 and may indicate that increased action potential frequency is not necessarily a prerequisite for increased pain perception. However, tonic action potential firing was more pronounced in IEM nociceptors, possibly indicating a lack of activity-dependent inhibition or even activity-dependent increase in excitability that could lead to pain attacks. Contrary to the study by Cao et al.,5 we did not find an increase in spontaneous action potential firing in our IEM nociceptors. The most likely explanations for this discrepancy are slight differences in the composition of differentiation and maturation media, which could affect ion channel expression. In addition, there are small differences in the ionic composition of the electrophysiological recording solutions between our study and the one by Cao et al. These small differences (mostly in the K+ gradient) lead to a small shift of the Nernst potential, thus potentially inducing changes in neuronal excitability.

We performed a detailed investigation of individual action potentials that revealed changes in several action potential characteristics induced by the IEM mutation: decreased threshold, increased amplitude, stronger afterhyperpolarization, as well as shorter and faster action potentials. Taken together, these findings suggest that due to the hyperpolarized voltage dependence, NaV1.7/I848T channels open sooner and in a more synchronized manner. This lowers the action potential threshold, meaning that a weak, potentially nonnoxious stimulus is enough to induce action potentials and might even trigger a pain attack.

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4.2. NaV1.7 plays a key role in action potential generation

It has previously been hypothesized that NaV1.7 is an amplifier of subthreshold stimuli, responsible for depolarizing the membrane potential to a point when NaV1.8 and 1.6 activate, which would then carry the upstroke of the action potential.2,13,14,34 Our results argue that NaV1.7 is unlikely to be a subthreshold channel because the slope of the subthreshold depolarization of the action potential is not changed in IEM nociceptors. In addition, blocking NaV1.7 by ProTx-II displayed a hyperpolarized voltage dependence of activation, suggesting that the remaining NaV isoforms open before NaV1.7 and are therefore likely to carry subthreshold depolarizations. This corresponds well with published activation V1/2 values for these NaV channels (NaV1.1,6,8 NaV1.2,8,22,44 NaV1.3,8,10 NaV1.6,4,8,36 and NaV1.926,41), all of which were reported to be more hyperpolarized compared with NaV1.7.10,11,18,24,40,43

As outlined in Figure 7, we show that the action potential upstroke depends significantly on NaV1.7 activity: action potential amplitude is increased, time to peak of the action potential is decreased, and the slope of the action potential upstroke is increased in IEM nociceptors. In addition, NaV1.7 seems to be crucial for the setting of the action potential threshold because the hyperpolarizing IEM mutation significantly reduces the neuron's firing threshold. This concept is supported by previous pharmacological evidence demonstrating that a specific NaV1.7 inhibitor affected the action potential threshold and rising phase of the DRG action potential but not the subthreshold phase.1

Figure 7

Figure 7

In patients suffering from IEM, the hyperpolarized voltage sensitivity of NaV1.7 could lead to a more synchronized activation of different NaV isoforms (Fig. 7). This would accelerate and increase the depolarization. As a result, it is possible that in IEM nociceptors, TTX-s isoforms other than NaV1.7 participate in the action potential upstroke. This increases the amplitude and the slope of the action potential. All these mechanisms together with the enhanced hyperpolarization support faster action potential propagation and potentially increased neurotransmitter release. These findings taken together argue that NaV1.7 is a key channel in defining action potential characteristics and in guaranteeing normal excitability of nociceptors. This crucial role of NaV1.7 is supported by the large number of mutations in NaV1.7 that are known to modify pain sensitivity in one way or another.15,17

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4.3. Erythromelalgia nociceptors display paradoxical activity-dependent speeding of conduction

In a previous study, we investigated conduction velocity of C-fiber nociceptors in an IEM patient carrying the same mutation using microneurography.30 In C-fiber nociceptors of this patient, normal activity-dependent slowing of conduction was turned into activity-dependent speeding. Based on these data, we predicted that the latter effect could be due to a more pronounced afterhyperpolarization in the neurons expressing the I848T mutation.30 This hypothesis is now confirmed in this study.

In that previous study, we also showed a slower resting conduction velocity in human IEM nociceptors in vivo.30 This could be due to an increased amount of inactivated NaVs at rest. Here, we show a slightly increased TTX-s NaV activity at RMP in IEM nociceptors (more channels open around −40 mV, Fig. 5C), which could lead to inactivation and therefore reduce conduction velocity. More importantly, the strongly enhanced hyperpolarization after an action potential in IEM nociceptors reverses this increased inactivation of resting TTX-s NaVs, creating a positive feedback loop and leading to faster-than-normal conduction in the later phase after an action potential, thus supporting our previous hypothesis. Combining the results of our previous clinical study with the present stem cell–based cellular study, we suggest that patients carrying the NaV1.7/I848T mutation suffer from increased pain sensation due to a reduced firing threshold in combination with an activity-dependent increase of axonal excitability.

In summary, we show that the NaV1.7/I848T mutation induces a hyperpolarized shift of voltage-dependent activation in a human patient-derived model system for IEM. This shift reduces the action potential threshold, enhances and speeds up the action potential upstroke, and increases hyperpolarization, inducing increased pain sensitivity in patients. These results from human nociceptors advance previous findings in human model systems and differ from previous reports on rodent sensory neurons, highlighting the impact of species differences. We therefore present iPS cell–derived neurons as a valuable tool for studying pain mechanisms and for developing and testing potential new pharmaceuticals. Finally, our results suggest a crucial role for NaV1.7 in action potential generation and propagation.

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Conflict of interest statement

A.M.R. and M.R. are shareholders and employees and M.R. is a founder and the director of Metrion Biosciences, Cambridge, United Kingdom. R.G. is an employee and W.W. is a cofounder of Cygenia GmbH, Aachen, Germany. The remaining authors have no conflicts of interest to declare.

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Acknowledgements

The authors thank B. Hoch and U. Gollan for excellent technical assistance, and Jürgen Winkler and Sonja Plötz for culture of fibroblasts.

This work was supported in part by the Ministry for Innovation, Science and Research of the German Federal State of North Rhine-Westphalia, Duesseldorf, Germany (S.S. and M.Z.), by the Bavarian Ministry of Education and Culture, Science, and the Arts in the framework of the Bavarian Molecular Biosystems Research Network, ForIPS, and the DFG-funded research training group GRK2162 (B.W.), GRK2416 (A.L.), and SFB 1158 (M.S.), by the Johannes and Frieda Marohn-Foundation (Win/2012 to B.W. and A.L.), by the Excellence Initiative of the German federal and state governments (Dean's Seed Fund of the RWTH Aachen University, A.L.), by the German Israeli Foundation (GIF, 1091-27.1/2010 to A.L.), by a grant from the Interdisciplinary Center for Clinical Research within the faculty of medicine at the RWTH Aachen University (B.N.), and the DFG grant NA 970 3 to 1 (B.N.) and LA 2740/3 to 1 (A.L.).

Author contributions: J.E. Meents planned and designed experiments, performed and analyzed patch-clamp experiments, interpreted the data, and wrote the manuscript. E. Bressan planned and designed experiments, generated and characterized iPS cells and iPS cell–derived neurons, performed and analyzed immunofluorescence and calcium imaging experiments, interpreted data, and contributed to writing the manuscript. S. Sontag planned and designed experiments, generated and characterized iPS cells, interpreted data, and wrote the manuscript. A. Foerster planned and designed experiments, performed and analyzed patch-clamp experiments, differentiated and maintained iPS cells, and contributed to writing the manuscript. P. Hautvast planned and designed experiments, performed and analyzed qPCR experiments, and differentiated and maintained iPS cells. C. Rösseler planned and designed experiments, and differentiated and maintained iPS cells. M. Hampl programmed analysis software for patch-clamp analysis, and supported design, analysis, and interpretation of patch-clamp experiments. H. Schüler performed and analyzed cytogenetic analysis. R. Goetzke performed Epi-Pluri-Score analysis. T.K.C. Le performed and analyzed patch-clamp experiments with ProTx-II, and interpreted data. I.P. Kleggetveit participated in assessment of the patients, took skin biopsies, and critically revised the manuscript. K. Le Cann performed current-clamp experiments and interpreted data. C. Kerth performed and analyzed patch-clamp experiments in HEK cells, and interpreted data. A.M. Rush discussed the results and critically revised the manuscript. M. Rogers discussed the results and critically revised the manuscript. Z. Kohl maintained fibroblast culture, helped to generate iPS cells, and interpreted data. M. Schmelz discussed the results and critically revised the manuscript. W. Wagner supported epigenetic characterization of iPS cells and critically revised the manuscript. E. Jørum is responsible for the recruitment of the IEM patients, their clinical assessment, and discussed data. B. Namer conceived the study, discussed experimental design, and critically revised the manuscript. B. Winner conceived the study, planned and designed experiments, and critically revised the manuscript. M. Zenke planned and designed experiments, interpreted data, and critically revised the manuscript. A. Lampert conceived the study, planned and designed experiments, analyzed and interpreted the data, and participated in writing and critically revising the manuscript.

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Supplemental digital content

Supplemental digital content associated with this article can be found online at http://links.lww.com/PAIN/A749.

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References

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Keywords:

Erythromelalgia; Pain; Nociceptor; Induced pluripotent stem cells; Voltage-gated sodium channel; Action potential firing; Patch-clamp; iPS cells; Inherited pain syndrome

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