The National Institutes of Health (NIH) minimum dataset for chronic low back pain (CLBP) was developed in response to the challenge of standardizing measurements across studies. Although reference values are critical in research on CLBP to identify individuals and communities at risk of poor outcomes such as disability, no reference values have been published for the Quebec (Canada) context. This study was aimed to: 1) provide reference values for the Canadian version of the NIH minimum dataset among individuals with CLBP in Quebec, both overall and stratified by gender, age, and pain impact stratification (PIS) subgroups; and 2) assess the internal consistency of the minimum dataset domains (pain interference (PI), physical function (PF), emotional distress/depression (EDD), sleep disturbance (SD), and PIS score). We included 2847 individuals living with CLBP who completed the baseline web survey of the Quebec Low Back Pain Study (age: 44.0±11.2 years, 48.1% women), and were recruited through social media and health care settings. The mean score was 6.1±1.8 for pain intensity. PI, PF, EDD, SD, and PIS scores were 12.9±4.1, 14.4±3.9, 9.8±4.4, 13.0±3.6, and 26.4±6.6, respectively. EDD showed floor effects. Good to excellent internal consistency was found overall and by language, gender, and age subgroups for all domains (alpha: 0.81-0.93) and poor to excellent internal consistency for PIS subgroups (alpha: 0.59–0.91). This study presents reference values and recommendations for using the Canadian version of the NIH minimum dataset for CLBP that can be useful for researchers and clinicians.