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Selectively targeting pain in the trigeminal system

Kim, Hyun Yeonga; Kim, Kihwana; Li, Hai Yinga; Chung, Gehoona; Park, Chul-Kyua; Kim, Joong Sooa; Jung, Sung Junb; Lee, Min Kyungc; Ahn, Dong Kukc; Hwang, Se Jind; Kang, Youngname; Binshtok, Alexander M.f; Bean, Bruce P.g; Woolf, Clifford J.f; Oh, Seog Baea,*

doi: 10.1016/j.pain.2010.02.016
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We tested whether it is possible to selectively block pain signals in the orofacial area by delivering the permanently charged lidocaine derivative QX-314 into nociceptors via TPRV1 channels. We examined the effects of co-applied QX-314 and capsaicin on nociceptive, proprioceptive, and motor function in the rat trigeminal system. QX-314 alone failed to block voltage-gated sodium channel currents (INa) and action potentials (APs) in trigeminal ganglion (TG) neurons. However, co-application of QX-314 and capsaicin blocked INa and APs in TRPV1-positive TG and dental nociceptive neurons, but not in TRPV1-negative TG neurons or in small neurons from TRPV1 knock-out mice. Immunohistochemistry revealed that TRPV1 is not expressed by trigeminal motor and trigeminal mesencephalic neurons. Capsaicin had no effect on rat trigeminal motor and proprioceptive mesencephalic neurons and therefore should not allow QX-314 to enter these cells. Co-application of QX-314 and capsaicin inhibited the jaw-opening reflex evoked by noxious electrical stimulation of the tooth pulp when applied to a sensory but not a motor nerve, and produced long-lasting analgesia in the orofacial area. These data show that selective block of pain signals can be achieved by co-application of QX-314 with TRPV1 agonists. This approach has potential utility in the trigeminal system for treating dental and facial pain.

aNational Research Laboratory for Pain, Dental Research Institute and Department of Physiology School of Dentistry, Seoul National University, Seoul 110–749, Republic of Korea

bDepartment of Physiology School of Medicine, Hanyang University, Seoul 133–791, Republic of Korea

cDepartment of Oral Physiology and Neurobiology School of Dentistry, Kyungpook National University, Daegu 700–412, Republic of Korea

dDepartment of Anatomy and Cell Biology College of Medicine, Hanyang University, Seoul 133–791, Republic of Korea

eDepartment of Neuroscience and Oral Physiology, Osaka University Graduate School of Dentistry, Osaka 565–0871, Japan

fNeural Plasticity Research Group, Massachusetts General Hospital & Harvard Medical School, Charlestown, MA 02129, USA

gDepartment of Neurobiology, Harvard Medical School, Boston, MA 02115, USA

*Corresponding author. Address: Department of Physiology, School of Dentistry, Seoul National University, 28–2 Yeongeon-Dong, Chongno-Ku, Seoul 110–749, Republic of Korea. Tel.: +82 2 740 8656; fax: +82 2 7625107.

E-mail address:odolbae@snu.ac.kr

Submitted August 21, 2009; revised December 5, 2009; accepted February 9, 2010.

© 2010 Lippincott Williams & Wilkins, Inc.
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