Spinal CGRP1 receptors contribute to supraspinally organized pain behavior and pain-related sensitization of amygdala neuronsAdwanikar, Hitaa; Ji, Guangchena; Li, Weidonga; Doods, Henrib; Willis, William D.a; Neugebauer, Volkera,*PAIN: November 2007 - Volume 132 - Issue 1 - p 53–66 doi: 10.1016/j.pain.2007.01.024 Research papers Buy Abstract Author InformationAuthors Article MetricsMetrics CGRP receptor activation has been implicated in peripheral and central sensitization. The role of spinal CGRP receptors in supraspinal pain processing and higher integrated pain behavior is not known. Here we studied the effect of spinal inhibition of CGRP1 receptors on supraspinally organized vocalizations and activity of amygdala neurons. Our previous studies showed that pain-related audible and ultrasonic vocalizations are modulated by the central nucleus of the amygdala (CeA). Vocalizations in the audible and ultrasonic range and hindlimb withdrawal thresholds were measured in awake adult rats before and 5–6 h after induction of arthritis by intra-articular injections of kaolin and carrageenan into one knee. Extracellular single-unit recordings were made from neurons in the latero-capsular division of the CeA (CeLC) in anesthetized rats before and after arthritis induction. CGRP1 receptor antagonists were applied to the lumbar spinal cord intrathecally (5 μl/min) 6 h postinduction of arthritis. Spinal administration of peptide (CGRP8-37, 1 μM) and non-peptide (BIBN4096BS, 1 μM) CGRP1 receptor antagonists significantly inhibited the increased responses of CeLC neurons to mechanical stimulation of the arthritic knee but had no effect under normal conditions. In arthritic rats, the antagonists also inhibited the audible and ultrasonic components of vocalizations evoked by noxious stimuli and increased the threshold of hindlimb withdrawal reflexes. The antagonists had no effect on vocalizations and spinal reflexes in normal rats. These data suggest that spinal CGRP1 receptors are not only important for spinal pain mechanisms but also contribute significantly to the transmission of nociceptive information to the amygdala and to higher integrated behavior. aDepartment of Neuroscience and Cell Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-1069, USA bBoehringer Ingelheim Pharma GmbH & Co. KG, D-88397 Biberach, Germany *Corresponding author. Tel.: +1 409 772 2124; fax: +1 409 772 2789. E-mail: email@example.com Submitted July 19, 2006; received in revised form and accepted January 26, 2007. © 2007 Lippincott Williams & Wilkins, Inc.