ArticlePressure and stretch mechanosensitivity of peripheral nerve fibres following local inflammation of the nerve trunkDilley, Andrew*; Lynn, Bruce; Pang, See Jye Author Information Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK *Corresponding author. Tel.: +44 207 679 6082; fax: +44 207 383 7005. E-mail: [email protected] Submitted April 13, 2005; revised July 29, 2005; accepted August 12, 2005. Pain: October 2005 - Volume 117 - Issue 3 - p 462-472 doi: 10.1016/j.pain.2005.08.018 Buy Metrics Abstract Patients with non-specific limb pain often show signs of nerve mechanosensitivity, i.e. local tenderness over nerve trunks and pain in response to limb movements that cause nerve stretch. In such patients a nerve lesion is not apparent, and it has been suggested that local neural inflammation may be a key factor. The present study examines the extent to which nerve fibres in regions of local inflammation respond to small stretches, and whether functional changes occur throughout the primary afferent neurone. A local neuritis was induced in adult rats by wrapping oxidised cellulose saturated in complete Freund's adjuvant (CFA) around the peroneal or sciatic nerves. A small cut was made in the perineurium of some of the peroneal lesioned animals. A- and C-fibre recordings were made 2–10 days post-surgery from filaments dissected proximal to the lesion. Local mechanosensitivity was assessed using a glass probe and by small stretches. Responses to stretch and local pressure were recorded in 7% of C- and 8% of A-fibres from the peroneal nerve following CFA treatment with the sheath opened. A smaller proportion of stretch sensitive fibres were seen in sciatic and peroneal nerves after CFA treatment alone (2% of C- and 3% of A-fibres), but such fibres were not seen in control preparations. The most responsive fibres fired to 3% stretch, which is within the range of nerve stretch seen during normal limb movements. Less than 1% of stretch sensitive fibres had peripheral fields, indicating that most had probably degenerated distally. © 2005 Lippincott Williams & Wilkins, Inc.