Antinociceptive effect of cardiopulmonary chemoreceptor and baroreceptor reflex activation in the ratSévoz-Couche, Caroline∗; Hamon, Michel; Laguzzi, RaulPAIN: September 2002 - Volume 99 - Issue 1 - p 71–81 doi: 10.1016/S0304-3959(02)00055-6 Article Buy SDC Abstract Author InformationAuthors Article MetricsMetrics The effect on the nociceptive tail-flick (TF) reflex of cardiopulmonary chemoreceptor and arterial baroreceptor activation, producing Bezold–Jarisch like- and baro-reflex responses, respectively, was analysed in lightly halothane-anaesthetized rats. Intra-cardiac administration of phenylbiguanide (5–100 μg/kg, into the right atrium) or veratrine (30–150 μg/kg, into the left ventricle), which both elicited the characteristic Bezold–Jarisch-like cardiovascular reflex responses (hypotension and bradycardia), produced a dose-dependent increase in TF latency. A similar inhibitory influence on the TF reflex was noted upon baroreflex activation by acute administration of phenylephrine (15–50 μg/kg i.v.) or aortic depressor nerve stimulation (100–400 μA). As expected from the involvement of local excitatory amino acid receptors in both vagally mediated cardiovascular reflex responses and inhibition of the TF reflex, microinjections of kynurenic acid (3 nmol/0.1 μl), an N-methyl-D-aspartate (NMDA) and non-NMDA receptor antagonist, into the nucleus tractus solitarius, prevented the cardiovascular responses as well as the concomitant increase in TF latency produced by cardiopulmonary chemoreceptor and baroreceptor stimulations. The present data show that induction of the cardiopulmonary chemoreceptor and baroreceptor reflexes produces an antinociceptive effect which can be assessed using the TF test, and that glutamate ionotropic receptors within the nucleus tractus solitarius mediate this effect. Neuropsychopharmacologie Moléculaire, Cellulaire et Fonctionnelle (INSERM U288), CHU Pitié-Salpêtrière, 91 Boulevard de l'Hôpital, 75634 Paris Cedex 13, France ∗Corresponding author. Tel.: +33-1-4077-9714; fax: +33-1-4077-9790 E-mail: email@example.com Submitted July 27, 2001; revised November 29, 2001; accepted February 19, 2002. © 2002 Lippincott Williams & Wilkins, Inc.