Secondary Logo

Share this article on:

Juvenile Tree Shrews Do Not Maintain Emmetropia in Narrow-band Blue Light

Gawne, Timothy J., PhD1*; Ward, Alexander H., PhD1; Norton, Thomas T., PhD, FAAO1

doi: 10.1097/OPX.0000000000001283
FEATURE ARTICLE – PUBLIC ACCESS

SIGNIFICANCE In spectrally broad-band light, an emmetropization mechanism in post-natal eyes uses visual cues to modulate the growth of the eye to achieve and maintain near emmetropia. When we restricted available wavelengths to narrow-band blue light, juvenile tree shrews (diurnal dichromatic mammals closely related to primates) developed substantial refractive errors, suggesting that feedback from defocus-related changes in the relative activation of long- and short-wavelength–sensitive cones is essential to maintain emmetropia.

PURPOSE The purpose of this study was to examine the effects of narrow-band ambient blue light on refractive state in juvenile tree shrews that had completed initial emmetropization (decrease from hyperopia toward emmetropia).

METHODS Animals were raised in fluorescent colony lighting until they began blue-light treatment at 24 days of visual experience, at which age they had achieved age-normal low hyperopia (mean ± SEM refractive error, 1.2 ± 0.5 diopters). Arrays of light-emitting diodes placed atop the cage produced wavelengths of 457 (five animals) or 464 nm (five animals), flickered in a pseudo-random pattern (temporally broad band). A third group of five animals was exposed to steady 464-nm blue light. Illuminance on the floor of the cage was 300 to 500 human lux. Noncycloplegic autorefractor measures were made daily for a minimum of 11 days and up to 32 days. Seven age-matched animals were raised in colony light.

RESULTS The refractive state of all blue-treated animals moved outside the 95% confidence limits of the colony-light animals' refractions. Most refractions first moved toward hyperopia. Then the refractive state decreased monotonically and, in some animals, passed through emmetropia, becoming myopic.

CONCLUSIONS From the tree shrew cone absorbance spectra, the narrow-band blue light stimulated both long-wavelength–sensitive and short-wavelength–sensitive cones, but the relative activation would not change with the refractive state. This removed feedback from longitudinal chromatic aberration that may be essential to maintain emmetropia.

1Department of Optometry and Vision Science, University of Alabama at Birmingham, Birmingham, Alabama *tgawne@gmail.com

Submitted: February 4, 2018

Accepted: May 22, 2018

Funding/Support: National Eye Institute (R21 EY02524; to TJG); UAB Tree Shrew Core; UAB Faculty Development Grant (to TJG); UAB Comprehensive Neuroscience Center; National Eye Institute (P30 EY003909); and EyeSight Foundation of Alabama.

Conflict of Interest Disclosure: None of the authors have reported a financial conflict of interest.

Author Contributions and Acknowledgments: Conceptualization: TJG, TTN; Data Curation: TJG, AHW, TTN; Formal Analysis: TJG, AHW, TTN; Funding Acquisition: TJG, TTN; Investigation: TJG, AHW, TTN; Methodology: TJG, AHW, TTN; Project Administration: TJG, AHW, TTN; Supervision: TJG, TTN; Validation: TTN; Visualization: TJG, TTN; Writing – Original Draft: TJG, AHW, TTN; Writing – Review & Editing: TJG, AHW, TTN.

The authors acknowledge the technical assistance of Regina Rab, Russell Veale, Alex Zotov, Wes Moore, and Eric Worthington.

Figure

Figure

It is well established that, in many species (including humans, monkeys, tree shrews, and chicks), an emmetropization mechanism operates in post-natal eyes using refractive error cues to dynamically adjust the eye's elongation rate to match the retinal location to that eye's focal plane.1–5 By fine tuning the eye's growth, the emmetropization mechanism achieves and actively maintains approximate emmetropia (typically, a slight hyperopia) as the eye continues to grow throughout the post-natal period and into adulthood.1,2,6–10 It is a failure of this mechanism to use visual cues to correctly guide the continued eye growth that is responsible for most human myopia. Therefore, understanding what these visual cues are and how the emmetropization mechanism interprets them is of high clinical relevance.

Animal experiments have determined that the emmetropization mechanism can respond appropriately not only to the presence of refractive error but also to its sign.1,4,6,9,10–18 Hyperopia (focal plane behind the retina), either naturally occurring or induced by placing a minus-power lens in front of the eye, causes the emmetropization mechanism to increase the axial (vitreous chamber) elongation rate. This moves the retina outward to the focal plane and reduces the hyperopia so the eye becomes nearly emmetropic while wearing the lens (“lens compensation”). Myopia (focal plane in front of the retina) induced by placing a positive (plus-power) lens in front of the eye produces slowed axial elongation if applied early in the post-natal period.12,16,18,19 The slowed axial elongation coupled with maturation of the optics gradually moves the retina to the focal plane so that the eye becomes emmetropic while wearing the lens and hyperopic with the plus lens removed.

Although the emmetropization mechanism can determine the sign of refractive error, the visual cues used to determine this are still poorly understood. In addition to optical defocus, many have suggested that longitudinal chromatic aberration could be used to determine the sign of refractive error because long-wavelength (“red”) light is focused farther from the cornea than is short-wavelength (“blue”) light.3,20–24 In most vertebrates, across the range of visible wavelengths, the short (blue) wavelengths focus 2 to 3 diopters closer to the cornea than do long (red) wavelengths,25 suggesting that longitudinal chromatic aberration would have a plausibly large magnitude to be useful as a cue to the emmetropization mechanism. In a visual environment containing a broad spectrum of wavelengths, if an eye is hyperopic, the blue wavelengths within an image will be in better focus compared with the red wavelengths, and the image contrast of the blue wavelengths will be greater than the image contrast of the red. This will be reversed if an eye is myopic. If the emmetropization mechanism uses this information as a signal to adjust the axial elongation rate to reduce refractive error, the image contrast will change so that, at emmetropia, a balance of image contrast at short versus long wavelengths will be achieved. Advantages of using longitudinal chromatic aberration as a cue are that the amount of longitudinal chromatic aberration is relatively constant across the surface of the retina (from center to periphery) and that its magnitude is relatively constant across both individuals and species. There is evidence that the emmetropization mechanism in chicks can make use of longitudinal chromatic aberration cues: one classic experiment using chicks showed that the eyes responded to simulations of longitudinal chromatic aberration–induced defocus as would be expected if longitudinal chromatic aberration was being used as a cue for emmetropization.26

In an environment that contains wavelengths across the visible spectrum (as in colony fluorescent lighting; Fig. 1A), refractive error could be signaled by optical defocus, higher-order aberrations (such as spherical aberration and off-axis astigmatism), longitudinal chromatic aberration, and possibly other cues, all providing information to the emmetropization mechanism that will change over time as refractive error is reduced. In the case of longitudinal chromatic aberration, ambient light containing a range of wavelengths will produce altered image contrast at long and short wavelengths as refractive error changes over time.

FIGURE 1

FIGURE 1

However, if only a narrow band of wavelengths is present, longitudinal chromatic aberration–related feedback will be absent (or reduced to a negligible level); no matter how refractive error changes, the relative effect of the narrow band of light on the short-, middle-, and long-wavelength–sensitive cones will remain essentially the same. In such a condition, it is possible that the emmetropization mechanism could use other refractive error cues to achieve and maintain emmetropia.29 However, it also is possible that, if longitudinal chromatic aberration feedback is absent, then the other refractive error cues become ineffective; that longitudinal chromatic aberration feedback is an essential cue.

We have previously found evidence suggesting that, without longitudinal chromatic aberration feedback, other refractive error cues become ineffective.28,30 When infant tree shrews (dichromatic mammals closely related to primates)31 were housed in an environment containing only narrow-band red light (Fig. 1C), which stimulates the long-wavelength–sensitive cones almost exclusively, the normal decrease in refraction from hyperopia to emmetropia did not occur. The growth of the eyes (primarily, vitreous chamber) was slowed, and the refractions remained substantially hyperopic. When juvenile and adolescent tree shrews that had achieved a nearly emmetropic refractive state were exposed only to the red light, the eyes also slowed their axial elongation and became significantly hyperopic. In this condition, hyperopic defocus was ineffective as a cue. However, when returned to colony fluorescent lighting containing a wide range of wavelengths (Fig. 1A), the hyperopia was quickly reduced by increased axial elongation.28,30 Similar results have been found in infant monkeys.32

If only narrow-band blue light is present, image contrast exists only for blue. However, in dichromatic mammals, the long-wavelength–sensitive cone pigment absorbs the short-wavelength light (albeit less effectively; Figs. 1B, C), so no blue wavelength can activate the short-wavelength–sensitive cones without also significantly activating the long-wavelength–sensitive cones (and, in trichromatic species, the middle-wavelength–sensitive cones as well). However, in narrow-band blue light, if refractive error changes over time, the ratio of excitation of all cones types remains constant, removing longitudinal chromatic aberration feedback.

In addition, in many species including humans,33 monkeys,34 and tree shrews,27 the density of short-wavelength–sensitive cones is sparse relative to that of longer wavelength cones (in tree shrews, short-wavelength–sensitive cones seem to be between 4 and 10% of the cone population, depending on location in the retina35), which likely would make it difficult to detect image defocus using the short-wavelength–sensitive cones. We had previously speculated that the emmetropization mechanism might use temporal flicker as a proxy for image sharpness in short wavelengths, signaling the emmetropization mechanism that the eye is too short and triggering axial elongation that would produce myopia. We found, in infant tree shrews, that flickering blue light created significant myopia.28 However, in steady blue light, eyes seemed to emmetropize normally.

Because the refractive state in the infant animals studied previously was changing rapidly (as in Fig. 2A, leftmost data points), decreasing from hyperopia toward emmetropia, we were concerned that the shifting normal baseline refractive state and normal variability might have made it difficult to accurately assess the effect of narrow-band blue light. Thus, in the present study, we reexamined the effect of flickering and steady, narrow-band blue light on the refractive state of juvenile tree shrews that had completed the initial phase of emmetropization, providing a more stable baseline against which to judge the effects of the blue light.

FIGURE 2

FIGURE 2

Back to Top | Article Outline

METHODS

Subjects and Experimental Groups

The tree shrews used in this study were raised by their mothers in the University of Alabama at Birmingham Tree Shrew Core until the time of the pedestal surgery (see discussion hereinafter) and the initiation of the experiment. This is approximately 6 weeks of age, which is generally the age of weaning. The colony is maintained on a 14-hour light-on/10-hour light-off cycle.36–38 Fluorescent lighting (F34CW RS WM ECO; General Electric Lighting, East Cleveland, OH) containing a wide range of wavelengths (Fig. 1A) provided illuminance of 100 to 300 lux on the floor of the cages. All procedures complied with the Association for Research in Vision and Ophthalmology statement for the use of animals in ophthalmic and visual research and were approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham.

Tree shrews are born with their eyes closed. It is presumed that the emmetropization mechanism does not start functioning until the eyes open and retinal image formation is possible. The first day that both eyes are open, which typically occurs approximately 3 weeks after birth, was designated as the first day of visual experience (DVE). Eye-opening status was checked daily, and all of our experimental manipulations were synchronized to this developmental time point. This is in accord with all previous published work from this site.

There were three blue light–treated groups (n = 5 per group) exposed to the following: flickering 457 ± 10-nm narrow-band light, flickering 464 ± 10-nm narrow-band light, and steady 464 ± 10-nm light. These were compared with a group (n = 7) of “normal” animals raised in the fluorescent colony light, data from which were reported in previous studies.28 All groups were balanced to include both males and females, and there were no siblings in any group. All four groups were maintained on the same 14-hour light/10-hour darkness schedule.

Blue-light exposure was started at 24 days of visual experience, an age when tree shrews have completed their initial steep descent from hyperopia toward emmetropization. At this point, the refractive state of colony-raised animals is relatively stable, typically around 1.2 diopters hyperopic, and is very slowly trending toward emmetropia over time (Fig. 2A). All blue-treated animals had blue-light exposure for a minimum of 11 days, but blue-light exposure was continued longer for 13 of the animals because it became apparent that a longer exposure was needed to achieve a clear understanding of the direction and magnitude of the refractive changes. Many were followed up until 35 days of visual experience, and one animal continued in blue light until 66 days of visual experience.

Back to Top | Article Outline

Blue-light Stimulators

Fig. 1A illustrates the spectrum of the colony fluorescent lighting in which all animals were initially raised and in which our normal animals remained. It has numerous sharp peaks but nonetheless spans the range of visible light and overlaps the absorbance spectra of both tree shrew cone types (Fig. 1B). Two different blue light–emitting diode arrays were used. One provided flickering blue light at a peak emission of 457 nm (Fig. 1C). It consisted of an array of five “Royal Blue” LXML-PR02-A900 light-emitting diodes (Luxeon Star LEDS, Brantford, Ontario, Canada) that were focused by a Carclo 44-degree circular beam optics onto a standard 60 × 60-cm lenticular diffuser mounted in a commercial metal fluorescent light fixture. This was placed on top of the cage (which was a cube 60 cm on a side). The light-emitting diodes were powered by a 3023-D-E-700, 700-mA controlled current “BuckPuck DC Driver” (LEDdynamics, Randolph, VT), which in itself was controlled by an Arduino microcontroller.

The other light-emitting diode array provided steady or flickering light at a peak emission of 464 nm (Fig. 1C). It was composed of six 50-cm-long strips of NFLS-RGBX2 high-power RGB flexible light strips (Superbright, St. Louis, MO), with only the blue light–emitting diodes activated. The strips were affixed to a white-painted piece of 60 × 60-cm plywood placed atop the cage. The light-emitting diodes were powered by a custom-designed MOSFET circuit controlled by an Arduino microprocessor. This array was used in our previous study.30

In the two flickering light groups (one at each blue wavelength), the light-emitting diodes were flickered around a mean luminance in a pseudo-random temporal pattern meant to mimic the natural time course of luminance on the retina as an animal moves around. This flicker is spectrally broad band, containing all frequencies less than 50 Hz; that is, it was not a single frequency. This temporal flicker pattern has been described previously and was used because, in infant tree shrews in our previous study, there seemed to be a difference between steady and flickering blue lights.28

The spectrum of the two blue wavelengths (Fig. 1C) was measured using a PhotoResearch LS670 spectrophotometer (Photo Research, North Syracuse, NY). The illuminance of the light at the cage floor was measured using an LX1330B digital illuminance meter (Hisgadget Inc., Union City, CA) and ranged from a mean of 300 to 500 human lux at the bottom center of the cage. The blue lights had a total irradiance power on the order of 2 W/m2 compared with direct sunlight at the Earth's surface, which is typically on the order of 1000 W/m2. In the blue part of the visible spectrum (400 to 500 nm), the blue lights were more than two orders of magnitude less intense than sunlight. It therefore was unlikely that the blue lights could be damaging to the retina.

In Fig. 1D, the relative absorbance by the short-wavelength–sensitive and long-wavelength–sensitive cones is shown. This was calculated by first multiplying the power at each wavelength by the wavelength to convert from power to photon count. Then, the dot product was taken with the cone absorption curve. The wavelengths emitted by the 464- and 457-nm blue light are both absorbed by the long-wavelength–sensitive cones and the short-wavelength–sensitive cones. However, the relative activation of the long-wavelength–sensitive cones by the 457-nm light-emitting diode is slightly less than that produced by the 464-nm light-emitting diodes. During blue-light treatment, the 457- or the 464-nm light was the sole illuminant present during the 14-hour lights-on period. Thus, the relative activation of the long-wavelength–sensitive versus the short-wavelength–sensitive cones would have been essentially constant over this time (our light stimuli were not truly monochromatic but were narrow band), as would the image contrast as sensed by these cones, regardless of changes in refractive state. This would prevent any substantial feedback from longitudinal chromatic aberration.

During blue-light exposure, the animals in all three groups were free to move around their cage and were provided with unrestricted access to food, water, and a dark-gray plastic nest tube with one open end, as were the normal colony-light animals. The amount of time that the animals spent in the nest tube was not recorded but did not seem to differ across groups. The cages also contained an elevated white shelf about halfway up from the bottom of the cage floor; light levels were typically approximately 40% higher on the shelf than on the cage floor. The cages had solid sides and backs, but the front and top were mesh. The tree shrews could look out the front of the cages at the room walls, typically 2 to 3 m away. Because the lighting arrays atop the cages were the only source of light in the room, this distant view was relatively dim. The floors of the cages were covered with newspaper. When the investigators entered the room, the animals were often found resting on the shelf but also sometimes were on the floor of the cage or inside their nest tube. No differences in behavior were noted across the blue-light and colony-light conditions.

Back to Top | Article Outline

Pedestal Installation

To consistently align the animals for awake refractive and axial component measures, a dental acrylic pedestal was installed on the skull of the experimental animals 2 to 3 days before treatment began at 24 days of visual experience, following procedures described previously.39 The pedestal in the normal group was installed at 10 days of visual experience. In brief, the animals were removed from their maternal home cage and anesthetized intramuscularly with 100 mg/kg ketamine and 7 mg/kg xylazine. After initial anesthesia but before the procedure began, they were given intraperitoneal atropine 0.27 mg/kg, intramuscular buprenorphine 0.02 mg/kg, and subcutaneous carprofen 5 mg/kg. Anesthesia was supplemented with 0.5 to 2.0% isoflurane as needed. After recovery from anesthesia, the animals were weaned and housed singly, or in pairs, in colony lighting until the start of blue-light treatment.

Back to Top | Article Outline

Refractive and Axial Measures

The refractive measures were made in awake, gently restrained animals with a Nidek ARK-700A infrared autorefractor (Marco Ophthalmic, Jacksonville, FL) using the pedestal for alignment, viewed on a video monitor.40 As in previous studies, noncycloplegic measures were made because atropine may interfere with emmetropization41,42 and because noncycloplegic measures have been shown to provide a valid estimate of a wide range of refractive states in tree shrews. Measured in the same animals, cycloplegic refractions for untreated and for myopic eyes were approximately 0.8 diopters hyperopic in comparison with noncycloplegic refractions (Norton TT, et al. IOVS 2000;41:ARVO E-Abstract 563)40 indicating the presence of a small tonic accommodation in treated and control eyes. All refractive values were corrected for the “small eye artifact”43 previously shown to be approximately +4 diopters in tree shrews.40

Refractive state was measured daily during treatment shortly after 9 AM, approximately 30 minutes after the lights were turned on. Animals were kept in darkness while being transported between their treatment cage and the measurement room. While the measurements were being made, the room was dimly illuminated only with blue light–emitting diodes similar to those in the treatment cage so that the animals did not have a period of visual stimulation that was different from the treatment conditions. The internal incandescent target light of the autorefractor was disabled to further avoid spurious visual stimulation.

Axial component dimensions were measured in all animals immediately before the start of treatment at 24 days of visual experience, after 11 days of treatment (35 days of visual experience), and at or near the end of blue-light treatment. Measures were made in awake, gently restrained animals with a LenStar LS-900 optical biometer (Haag-Streit, Mason, OH). This system uses optical low-coherence optical interferometry and has been found to typically give similar dimensions to those using other techniques such as ultrasonography, but with better repeatability.44 The LenStar provides and stores waveforms with peaks that correspond to the front and back of the cornea, front and back of the lens, front and back of the retina, and front and back of the choroid.45 Offline, the retinal cursors were manually moved to provide measures of vitreous chamber depth and choroidal thickness. All axial components (including choroid) were summed to provide a measure of axial length.

Back to Top | Article Outline

Statistical Analysis

Refractive and axial component measures were summarized in Excel spreadsheets (Excel for Mac, version 16.13.1; Microsoft, Redmond, WA). For all animals, the left and right eyes generally had similar refractions. Therefore, the data from the two eyes were averaged for each animal and treated as a single data point.46 Refractive measures were plotted as a function of time (days of visual experience). Comparisons were made between the normal animals and the three blue groups using a two-way ANOVA (P < .05) with repeated measures using IBM SPSS Statistics 24 (IBM, Armonk, NY). We also performed independent one-way ANOVAs on the refractive data both just before the start of treatment (24 days of visual experience) and after 11 days of treatment (35 days of visual experience), two time points when all animals were measured. To explore the variability of the refractive state over time, we first calculated the mean refraction over time for the seven normal animals. We then calculated the 95% confidence interval around this mean as simply ±2 standard deviations. It is important to remember that this is not the confidence interval for the mean: this is the area in which one would expect 95% of all individual data points to fall.

In most blue-treated animals, the refractive state change over time eventually became nearly linear. The linear region was determined for each animal, and the slope of the refractive change was calculated with the Excel regression function using all data points between the start and the end of this linear region.

Back to Top | Article Outline

RESULTS

Refractive Measures

The refractive measures over time for the individual animals in the colony-light and blue-treatment groups are shown in Fig. 2. As found previously, the refractions of the colony-reared animals (Fig. 2A) were hyperopic and variable when measurements began at 11 days of visual experience.47,48 From 11 to 24 days of visual experience, the refractions decreased toward emmetropia and became less variable. From 24 to 60 days of visual experience, the refractive state of each animal slowly declined toward emmetropia, and the variability across animals continued to decrease. At 35 days of visual experience, the range of the colony-light refractions (maximum individual animal refraction minus minimum refraction) was 1.2 diopters. By 60 days of visual experience, the range of refractive states across animals was 0.7 diopters. As shown in Fig. 2A, the refractions of the seven colony-light animals were within the 95% confidence intervals for the group at all measurements after 24 days of visual experience.

When blue-light exposure began at 24 days of visual experience, there was no significant difference in refraction across the four groups (one-way ANOVA, F 3,18 = 0.29, P = .83). However, during blue-light treatment, the refractions of all animals in the three groups diverged from those of the colony-light animals. Figs. 2B to D show the individual animals of each blue treatment group and the 95% confidence interval of the colony-light group. The refractive state of many (13/15) of the blue-light animals moved in the hyperopic direction within 1 to 4 days after the onset of treatment. The duration and magnitude of the hyperopic shift were highly variable; for some animals, it lasted only 1 day, and in others, it continued for up to 10 days. For the animals that showed a brief hyperopic shift, the refractive state initially remained within the 95% confidence intervals. For those in which the hyperopic shift continued for more than 3 days, the refractive state eventually became more hyperopic than the 95% confidence intervals by 0.5 to 4.5 diopters.

After a variable period, the refractions of all blue-treated animals began to decrease in the direction of myopia; this occurrence varied from animal to animal. For some, this occurred within 1 to 4 days after the onset of blue-light exposure, and for others, the decrease did not begin until up to 10 days. This includes two animals in the 457-nm blue flicker group that developed substantial hyperopia and were followed up for only 11 days. Refractions peaked after 7 and 10 days. For the remaining 13 animals, during the period of myopic drift, the slope of the daily decrease in refractive state (from maximum refraction to last measure) was −0.3 ± 0.1 diopters/d (mean ± SEM). In six animals, members of all three blue-treatment groups, the refractions first became more hyperopic than the 95% confidence intervals and then more myopic, passing through emmetropia without an apparent change in slope. Two additional animals' refractions were decreasing slowly but were still hyperopic when treatment ended at 50 and 66 days of visual experience.

All animals were exposed to blue light for at least 11 days (until 35 days of visual experience). At this time point, the refractive states of the four groups were significantly different from each other (one-way ANOVA, F 3,18 = 9.31, P = .001). The mean (± SEM) refraction for the normal group was 1.0 ± 0.2 diopters; for the flickering 464-nm group, it was −2.6 ± 1.2 diopters; for the flickering 457-nm group, it was 3.4 ± 0.9 diopters; and for the steady 464-nm group, it was 0.7 ± 0.8 diopters. However, the group refractive states at this time point fail to convey the diversity of the response trajectories of the individual animals in blue light. Increased variability and departure from the colony-light group were the most prominent results.

Generally, during blue-light treatment, the two eyes followed the same trajectory. However, at the end of blue treatment (mean, 49 days of visual experience), the absolute difference between the right and left eyes for the 15 blue-treated animals was 1.2 ± 0.2 diopters. This was significantly greater than the interocular difference of the colony animals at 39 days of visual experience (0.4 ± 0.1 diopters) or at 52 days of visual experience (0.4 ± 0.1 diopters) (independent t test, P < .05) and suggests that guidance needed to coordinate the refraction of the two eyes was reduced or absent.

Back to Top | Article Outline

Ocular Component Dimensions

Axial component dimensions were measured on all animals at or near the last day of blue-light exposure (which varied from 35 to 66 days of visual experience, see Methods). Fig. 3 shows that the vitreous chamber depth was correlated with refractive state. Eyes with a larger vitreous chamber were all myopic. Vitreous chamber depth was smaller in eyes with less myopia and smaller still in the four animals that were hyperopic at the end of their (short) blue-light treatment period. This correlation had an R 2 of 0.60 and a statistically significant (P < .05) slope of −0.015 mm per diopter. None of the other ocular component dimensions showed a significant relationship to refractive state.

FIGURE 3

FIGURE 3

Back to Top | Article Outline

DISCUSSION

In Narrow-band Blue Light, Juvenile Tree Shrew Eyes Did Not Maintain Emmetropia

In the present study, the refractive state of the juvenile tree shrews, whose eyes were nearly emmetropic at the start of treatment, became unstable with continued exposure to blue light—whether flickering or steady. The refractions of all 15 blue-treated animals moved outside the 95% confidence intervals of the colony-light animals. Other cues for refractive error, such as optical defocus, did not allow for the emmetropization mechanism to keep the eyes within the reference range of refractive state. The refractive state of most animals in all three blue-treatment groups first moved in the hyperopic direction by varying amounts in the first few days after the onset of blue treatment. After a variable period, the refractive state moved toward myopia. In all three groups, some animals that had become hyperopic then became myopic, passing through emmetropia. For those animals, optical or other cues were insufficient to signal the emmetropization mechanism to stop the decrease in refractive state so that it remained at emmetropia. It seems that, in the absence of a wide range of wavelengths that can provide substantial differential wavelength (longitudinal chromatic aberration) cues, juvenile tree shrews are unable to maintain emmetropia, even though the blue light also activated long-wavelength sensitive cones (Fig. 1D) which could provide defocus information.

In the present study, we found that both flickering and steady, narrow-band blue lights had very similar effects. In our previous study using infant tree shrews,28 we found that flickering blue light produced myopia, whereas steady blue light allowed for normal emmetropization (although with increased variability). Although it is possible that the effects of steady and flickering blue lights change with age, it seems more likely that by studying older animals in which the refractive state was relatively stable and less variable we were able in the present study to more accurately assess the effect of flickering and steady blue lights and found that there was little difference in the refractive responses to these conditions.

Back to Top | Article Outline

Types of Instability of Refractive State in Blue Light

The three salient effects of blue-light exposure in this study are as follows: the instability of the refractions, the variable initial hyperopic shift that occurred in most animals, and the eventual myopic shift in refractive state. In the tree shrews raised in colony light, like others observed in past studies,30,47 the emmetropization mechanism produced a highly consistent refractive state that was maintained within a narrow range. The instability of the refractions in the blue-light groups suggests that the feedback (error signals) normally used by the emmetropization mechanism to maintain emmetropia was greatly reduced or set to zero, allowing the eyes to drift away from age-appropriate refractions. The divergence of the refractions of the right and left eyes in the blue-treated animals (relative to normal animals) is also consistent with the notion that feedback to the emmetropization mechanism was reduced or absent.

The reason for the initial hyperopic shift observed in many animals is unknown. However, we speculate that differences in illuminance between the colony light and the blue light may have been a factor. The colony light, measured as 100 to 300 lux with a meter calibrated for humans, contained light at many wavelengths. The blue light, measured as 300 to 500 lux with the same meter, may have seemed brighter to the emmetropization mechanism (especially if this mechanism heavily weights luminance using the blue-sensitive intrinsically photosensitive retinal ganglion cells, shown to be present in tree shrew retinas49). This may have had same effect as did increasing the illuminance of colony lighting to approximately 975 lux in a previous study, which also produced a small transient hyperopic shift.30 Increasing the colony illuminance to approximately 15,000 lux also produced a hyperopic shift in a group of colony-raised juvenile animals (unpublished data). Why the hyperopic shift was so variable across animals is undetermined.

A myopic drift in the refractions was the dominant long-term effect. If observations in blue light had continued longer, it seems likely that all animals would have become myopic. The myopic drift in blue light resembles the myopic effect of dark treatment in juvenile tree shrews that had experienced colony lighting until they were similar age to the animals in the present study.48 During 10 days in complete darkness, where no visual guidance was available, the refractive state of all eyes shifted in the myopic direction by variable amounts. The slope of the refractive shift was −0.4 ± 0.1 diopters/d, slightly greater than the −0.3 ± 0.1 diopters/d observed in the blue-light animals. Also, the absolute difference in refraction between the right and left eyes increased during dark treatment. The similar myopic drift and reduced interocular coordination in the blue light– and dark-treated animals suggest that feedback to guide the emmetropization mechanism may have been reduced or absent during exposure to the blue light, despite the presence of other refractive error cues. Both results also demonstrate that the maintenance of emmetropia in juvenile eyes depends on the continued functioning of the emmetropization mechanism.

The variable trajectories of the refractions in this study may help to explain the reports of the variable effect of blue light in other species. Guinea pigs have been reported to become consistently hyperopic in blue light.50,51 Some studies have suggested that chickens do not require wavelength cues for emmetropization and will emmetropize normally under blue light20,24 or that blue light tends to produce hyperopia.23 In the present study, most animals were maintained in blue light until the refractive trajectory was clear. Importantly, refractive state was measured daily rather than only at the start and end of treatment. If we had compared the refractive state of the animals only at the start and end of a fixed, short period, such as the 11 days that was originally intended (before we realized how unstable the refractions were over time), we would have reported increased variability but would have missed the overall myopic effect. If we had chosen even shorter treatment periods, we would have reported that blue light produced a hyperopic shift in refraction. We suggest that, when exploring the effects of new types of visual stimuli on refractive state, it is critical to not use fixed, short treatment period, comparing only the starting and ending refractions, but rather to follow the refractive development of experimental animals with multiple measures over a sufficient period to determine the overall effect.

Back to Top | Article Outline

Effect of Limited Wavelength Cues at Short and Long Wavelengths

In addition to examining the effect of narrow-band blue light, we also have studied how exposure to narrow-band long-wavelength (red) light affects emmetropization.28,30,52 The effect of the red light is both similar to and different from the effect of the blue. Both are similar in that the eyes do not remain emmetropic if only a restricted band of wavelengths (±10 nm) is present. In both conditions, a refractive error develops that is not corrected by other defocus cues. It seems that the presence of a range of wavelengths that permit feedback from longitudinal chromatic aberration cues may be essential for the emmetropization mechanism to perform normally. The most evident difference is that, in narrow-band red light, infant, juvenile, and adolescent tree shrews became significantly hyperopic compared with colony-light animals.30 In contrast, the blue light produced an eventual myopic drift.

We have speculated that the very consistent red-induced hyperopia occurred because the red light only stimulates long-wavelength–sensitive cones. Fig. 1C shows the illuminance spectrum provided by red light–emitting diodes that were used in previous studies.28,30,52 The red wavelengths were very far removed from the short-wavelength–sensitive cone absorption curve for tree shrews27; the effect on the short-wavelength–sensitive cones is between 5 and 6 log units less than on the long-wavelength–sensitive cones (Fig. 1D). Thus, the red light almost exclusively activated the long-wavelength–sensitive cones and the post-receptoral retinal circuitry with long-wavelength–sensitive cone input. This may have caused the emmetropization mechanism to signal that the eye was too long for its optics because image contrast at red was present, but image contrast at the blue end of the spectrum was not detected, a condition that would be associated with the blue wavelengths being very far out of focus. It would follow that the emmetropization mechanism would slow the rate of axial elongation, producing eyes that are hyperopic. It may be that the narrow-band blue light, because it stimulates the short-wavelength–sensitive and the long-wavelength–sensitive cones, cancels the hyperopic effect of long-wavelength–sensitive cone activation.

Although exposure to an environment that contains only a narrow band of long or short wavelengths seems to reduce the ability of other visual cues to provide effective input to the emmetropization mechanism, these cues can still affect the emmetropization mechanism. We have previously shown that monocular form deprivation in red light produces myopia as does minus lens wear, although the amount of lens-induced myopia was less than in colony light (Ward AH, et al. IOVS 2017;58:ARVO E-Abstract 449). Thus, the ability of other cues is not completely disrupted in narrow-band light.

Back to Top | Article Outline

Accommodation in Narrow-band Light

Accommodation and emmetropization both have the same basic task: to evaluate focus and adjust the optics appropriately to maintain good focus (although on very different time scales). One might therefore expect that these two systems would use the same optical cues. In general, human subjects can correctly accommodate to scenes lit by narrow-band light of different wavelengths. Because longer wavelengths focus farther back than shorter wavelengths, there is more accommodation needed to clear the image if only long wavelengths are present compared with the amount of accommodation needed in short wavelengths, so that the image is optimally focused.23 However, accommodation in narrow-band light is slower, and the “gain” of accommodation is less than for images viewed in broad-band illumination.53 Also, there is a paradoxical effect for wavelengths less than 430 nm, where human subjects start to accommodate more rather than less.54 Thus, the accommodation mechanisms can use wavelength cues,55 but it does not seem that, under most conditions, differential wavelength cues are absolutely required for accommodation in humans. (We have not, however, measured accommodation in tree shrews, which is a limitation of this study.)

However, accommodation makes use of central processing mechanisms and can integrate information on depth from stereopsis, motion parallax, vergence angle, and so on. In addition, accommodation could potentially use a “hunting” strategy, wherein if changing accommodation in one direction makes focus worse, it only needs to go the other way. In contrast, the emmetropization mechanism probably functions primarily within the eye using local retinal processing. This may explain why both accommodation and emmetropization may be able to use wavelength (longitudinal chromatic aberration) cues; for accommodation, these are optional, but for emmetropization, they are essential.

Back to Top | Article Outline

Intrinsically Photosensitive Retinal Ganglion Cells

Tree shrew retinas have been found to have melanopsin-containing intrinsically photosensitive retinal ganglion cells.49 These cells receive input from photoreceptors via bipolar cells, but they are also directly activated by light via the blue-sensitive photopigment, melanopsin. They seem to be responsible for many non–image-forming functions of the visual system such as the pupillary light reflex and entraining circadian rhythms to the pattern of light and dark.56 Because the photopigment is distributed widely in dendrites, the receptive fields of these cells are large, so their activity cannot be involved in the detection of image focus. However, these cells could be involved in regulating the state of light adaptation within the retina, which could possibly affect emmetropization. The narrow-band blue light presumably could activate the intrinsically photosensitive retinal ganglion cells and perhaps affect emmetropization, but any effect is unknown at this time.

Back to Top | Article Outline

Tree Shrews as a Model for Human Emmetropization

Although tree shrews are a dichromatic species and most humans are trichromatic, we suggest that the results in tree shrews are likely to provide useful information for human emmetropization. Tree shrews are diurnal mammals, with good visual acuity, which are very closely related to primates.31 Because emmetropization is a fundamental issue for any animal with a camera-type eye that needs good visual acuity, one might expect this mechanism to be evolutionarily ancient and conserved across species with substantial retinal cones. Indeed, trichromacy evolved only recently in the primate lineage; dichromacy has been suggested to be the baseline state of color vision for all mammals including primates.57 Dichromacy is adequate for emmetropization, both in dichromatic species and in humans who are red/green color blind and thus have only the same basic long- versus short-wavelength color vision system as tree shrews; there is no evidence that these individuals emmetropize differently from trichromatic humans. One article suggested that some forms of human red/green color blindness are actually protective against myopia.58 It seems that having two cone types with wavelength absorption profiles that can use information provided by longitudinal chromatic aberration is essential for normal emmetropization in mammals. Further experiments in tree shrews and monkeys will either strengthen or weaken this hypothesis.

Back to Top | Article Outline

Relationship to Clinical Studies Using Blue Light

Blue light has been much in the news lately, most notably as regards its possible effects on circadian rhythms and sleep quality.59 However, blue light has also been suggested to be important for emmetropization. One group claims that lenses and windows that block blue light of a wavelength of less than 400 nm can be myopiagenic,60 although this is currently quite controversial.61 There are also many commercially available blue light–blocking glasses and many claims that they are made for these, but there is hardly any peer-reviewed literature on this topic. The only relationship of these studies and products with this study is the use of the word “blue.” The only clear clinical prediction to be derived from our results is that it would probably be unwise to raise children in a constant narrow-band blue-light environment. However, our findings suggest that the presence of a wide range of wavelengths in the environment is essential for emmetropization in tree shrews and possibly also in humans. Narrow-band light radically interferes with emmetropization. The question then arises: could artificial ambient light that is not strictly narrow band but that is still limited in the range of wavelengths compared with natural lighting interfere with emmetropization in a way that is less extreme than found here but still clinically significant? Further research will be needed to answer this question.

Back to Top | Article Outline

CONCLUSIONS

The results of the present study using narrow-band blue light, taken with our previous results with narrow-band red light, have established that an environment containing only a narrow band of wavelengths is insufficient to establish and maintain emmetropia in tree shrews. In both red and blue, it seems that other visual cues, such as optical defocus, cannot provide information to the emmetropization that is sufficient for normal functioning. Rather, the presence of a wide range of wavelengths may be essential to maintain emmetropia. The studies in red and blue light also have shown that future studies into the nature of the visual cues that guide emmetropization would benefit from following refractive development over time with frequent measures rather than only at arbitrarily defined start and stop points.

Back to Top | Article Outline

REFERENCES

1. Wildsoet CF. Active Emmetropization—Evidence for Its Existence and Ramifications for Clinical Practice. Ophthalmic Physiol Opt 1997;17:279–90.
2. Norton TT. Animal Models of Myopia: Learning How Vision Controls the Size of the Eye. ILAR J 1999;40:59–77.
3. Schaeffel F, Howland HC. Properties of the Feedback Loops Controlling Eye Growth and Refractive State in the Chicken. Vision Res 1991;31:717–34.
4. Mutti DO, Mitchell GL, Jones LA, et al. Axial Growth and Changes in Lenticular and Corneal Power during Emmetropization in Infants. Invest Ophthalmol Vis Sci 2005;46:3074–80.
5. Smith EL 3rd, Hung LF, Huang J, et al. Effects of Optical Defocus on Refractive Development in Monkeys: Evidence for Local, Regionally Selective Mechanisms. Invest Ophthalmol Vis Sci 2010;51:3864–73.
6. Wallman J, Winawer J. Homeostasis of Eye Growth and the Question of Myopia. Neuron 2004;43:447–68.
7. Schaeffel F, Feldkaemper M. Animal Models in Myopia Research. Clin Exp Optom 2015;98:507–17.
8. Smith EL 3rd, Hung LF, Arumugam B. Visual Regulation of Refractive Development: Insights from Animal Studies. Eye (Lond) 2014;28:180–8.
9. Norton TT, Amedo AO, Siegwart JT Jr. The Effect of Age on Compensation for a Negative Lens and Recovery from Lens-induced Myopia in Tree Shrews (Tupaia glis belangeri). Vision Res 2010;50:564–76.
10. Amedo AO, Norton TT. Visual Guidance of Recovery from Lens-induced Myopia in Tree Shrews (Tupaia glis belangeri). Ophthalmic Physiol Opt 2012;32:89–99.
11. Schaeffel F, Glasser A, Howland HC. Accommodation, Refractive Error and Eye Growth in Chickens. Vision Res 1988;28:639–57.
12. Irving EL, Sivak JG, Callender MG. Refractive Plasticity of the Developing Chick Eye. Ophthalmic Physiol Opt 1992;12:448–56.
13. Hung LF, Crawford ML, Smith EL. Spectacle Lenses Alter Eye Growth and the Refractive Status of Young Monkeys. Nat Med 1995;1:761–5.
14. Smith EL III, Hung LF. The Role of Optical Defocus in Regulating Refractive Development in Infant Monkeys. Vision Res 1999;39:1415–35.
15. Shen W, Sivak JG. Eyes of a Lower Vertebrate Are Susceptible to the Visual Environment. Invest Ophthalmol Vis Sci 2007;48:4829–37.
16. Metlapally S, McBrien NA. The Effect of Positive Lens Defocus on Ocular Growth and Emmetropization in the Tree Shrew. J Vis 2008;8:1–12.
17. Troilo D, Totonelly K, Harb E. Imposed Anisometropia, Accommodation, and Regulation of Refractive State. Optom Vis Sci 2009;86:E31–9.
18. Howlett MH, McFadden SA. Spectacle Lens Compensation in the Pigmented Guinea Pig. Vision Res 2009;49:219–27.
19. Siegwart JT Jr., Norton TT. Binocular Lens Treatment in Tree Shrews: Effect of Age and Comparison of Plus Lens Wear with Recovery from Minus Lens–induced Myopia. Exp Eye Res 2010;91:660–9.
20. Rohrer B, Schaeffel F, Zrenner E. Longitudinal Chromatic Aberration and Emmetropization: Results from the Chicken Eye. J Physiol 1992;449:363–76.
21. Rucker FJ, Kruger PB. Cone Contributions to Signals for Accommodation and the Relationship to Refractive Error. Vision Res 2006;46:3079–89.
22. Rucker FJ, Wallman J. Cone Signals for Spectacle-lens Compensation: Differential Responses to Short and Long Wavelengths. Vision Res 2008;48:1980–91.
23. Seidemann A, Schaeffel F. Effects of Longitudinal Chromatic Aberration on Accommodation and Emmetropization. Vision Res 2002;42:2409–17.
24. Wildsoet CF, Howland HC, Falconer S, et al. Chromatic Aberration and Accommodation: Their Role in Emmetropization in the Chick. Vision Res 1993;33:1593–603.
25. Mandelman T, Sivak JG. Longitudinal Chromatic Aberration of the Vertebrate Eye. Vision Res 1983;23:1555–9.
26. Rucker FJ, Wallman J. Chick Eyes Compensate for Chromatic Simulations of Hyperopic and Myopic Defocus: Evidence that the Eye Uses Longitudinal Chromatic Aberration to Guide Eye-growth. Vision Res 2009;49:1775–83.
27. Petry HM, Harosi FI. Visual Pigments of the Tree Shrew (Tupaia belangeri) and Greater Galago (Galago crassicaudatus): A Microspectrophotometric Investigation. Vision Res 1990;30:839–51.
28. Gawne TJ, Siegwart JT Jr., Ward AH, et al. The Wavelength Composition and Temporal Modulation of Ambient Lighting Strongly Affect Refractive Development in Young Tree Shrews. Exp Eye Res 2017;155:75–84.
29. Rucker F, Henriksen M, Yanase T, et al. The Role of Temporal Contrast and Blue Light in Emmetropization. Vision Res 2017; Aug 1:e-pub ahead of print: doi 10.1016/j.visres.2017.07.003.
30. Gawne TJ, Ward AH, Norton TT. Long-wavelength (Red) Light Produces Hyperopia in Juvenile and Adolescent Tree Shrews. Vision Res 2017;140:55–65.
31. Luckett WP. Comparative Biology and Evolutionary Relationships of Tree Shrews. New York: Plenum Press; 1980.
32. Smith EL III, Hung LF, Arumugam B, et al. Effects of Long-wavelength Lighting on Refractive Development in Infant Rhesus Monkeys. Invest Ophthalmol Vis Sci 2015;56:6490–500.
33. Roorda A, Williams DR. The Arrangement of the Three Cone Classes in the Living Human Eye. Nature 1999;397:520–2.
34. Roorda A, Metha AB, Lennie P, et al. Packing Arrangement of the Three Cone Classes in Primate Retina. Vision Res 2001;41:1291–306.
35. Muller B, Peichl L. Topography of Cones and Rods in the Tree Shrew Retina. J Comp Neurol 1989;282:581–94.
36. McBrien NA, Norton TT. The Development of Experimental Myopia and Ocular Component Dimensions in Monocularly Lid-sutured Tree Shrews (Tupaia belangeri). Vision Res 1992;32:843–52.
37. Guo L, Frost MR, He L, et al. Gene Expression Signatures in Tree Shrew Sclera in Response to Three Myopiagenic Conditions. Invest Ophthalmology Vis Sci 2013;54:6806–19.
38. He L, Frost MR, Siegwart JT Jr., et al. Gene Expression Signatures in Tree Shrew Choroid during Lens-induced Myopia and Recovery. Exp Eye Res 2014;123:56–71.
39. Siegwart JT Jr., Norton TT. Goggles for Controlling the Visual Environment of Small Animals. Lab Anim Sci 1994;44:292–4.
40. Norton TT, Wu WW, Siegwart JT Jr. Refractive State of Tree Shrew Eyes Measured with Cortical Visual Evoked Potentials. Optom Vis Sci 2003;80:623–31.
41. McKanna JA, Casagrande VA. Atropine Affects Lid-suture Myopia Development. Doc Ophthalmol Proc Ser 1981;28:187–92.
42. McBrien NA, Moghaddam HO, Reeder AP. Atropine Reduces Experimental Myopia and Eye Enlargement via a Nonaccommodative Mechanism. Invest Ophthalmol Vis Sci 1993;34:205–15.
43. Glickstein M, Millodot M. Retinoscopy and Eye Size. Science 1970;168:605–6.
44. Penha AM, Burkhardt E, Schaeffel F, et al. Ultrasonography and Optical Low-coherence Interferometry Compared in the Chicken Eye. Optom Vis Sci 2012;89:916–21.
45. Ward AH, Siegwart JT Jr., Frost MR, et al. The Effect of Intravitreal Injection of Vehicle Solutions on Form Deprivation Myopia in Tree Shrews. Exp Eye Res 2016;145:289–96.
46. Ederer F. Shall We Count Numbers of Eyes or Numbers of Subjects? Arch Ophthalmol 1973;89:1–2.
47. Norton TT, McBrien NA. Normal Development of Refractive State and Ocular Component Dimensions in the Tree Shrew (Tupaia belangeri). Vision Res 1992;32:833–42.
48. Norton TT, Amedo AO, Siegwart JT Jr. Darkness Causes Myopia in Visually Experienced Tree Shrews. Invest Ophthalmol Vis Sci 2006;47:4700–7.
49. Johnson EN, Westbrook T, Shayesteh R, et al. Distribution and Diversity of Intrinsically Photosensitive Retinal Ganglion Cells in Tree Shrew. J Comp Neurol 2017; Dec 14:epub ahead of print:doi 10.1002/cne.24377.
50. Liu R, Qian YF, He JC, et al. Effects of Different Monochromatic Lights on Refractive Development and Eye Growth in Guinea Pigs. Exp Eye Res 2011;92:447–53.
51. Jiang L, Zhang S, Schaeffel F, et al. Interactions of Chromatic and Lens-induced Defocus during Visual Control of Eye Growth in Guinea Pigs (Cavia porcellus). Vision Res 2014;94:24–32.
52. Ward AH, Norton TT, Huisingh CE, et al. The Hyperopic Effect of Narrow-band Long-wavelength Light in Tree Shrews Increases Non-linearly with Duration. Vision Res 2018;146-147:9–17.
53. Aggarwala KR, Nowbotsing S, Kruger PB. Accommodation to Monochromatic and White-light Targets. Invest Ophthalmol Vis Sci 1995;36:2695–705.
54. Graef K, Schaeffel F. Control of Accommodation by Longitudinal Chromatic Aberration and Blue Cones. J Vis 2012;12:1–14.
55. Rucker FJ, Kruger PB. The Role of Short-wavelength Sensitive Cones and Chromatic Aberration in the Response to Stationary and Step Accommodation Stimuli. Vision Res 2004;44:197–208.
56. Do MT, Yau KW. Intrinsically Photosensitive Retinal Ganglion Cells. Physiol Rev 2010;90:1547–81.
57. Jacobs GH. The Distribution and Nature of Colour Vision among the Mammals. Biol Rev Camb Philos Soc 1993;68:413–71.
58. Qian YS, Chu RY, He JC, et al. Incidence of Myopia in High School Students with and without Red-green Color Vision Deficiency. Invest Ophthalmol Vis Sci 2009;50:1598–605.
59. Hatori M, Gronfier C, Van Gelder RN, et al. Global Rise of Potential Health Hazards Caused by Blue Light–induced Circadian Disruption in Modern Aging Societies. NPJ Aging Mech Dis 2017;3:9.
60. Torii H, Kurihara T, Seko Y, et al. Violet Light Exposure Can Be a Preventive Strategy Against Myopia Progression. EBioMedicine 2017;15:210–9.
61. Schaeffel F, Smith EL 3rd. Inhibiting Myopia by (Nearly) Invisible Light? EBioMedicine 2017;16:27–8.
© 2018 American Academy of Optometry