Microbial contamination of contact lenses during wear is closely associated with ocular inflammation such as contact lens–induced acute red eye (CLARE),1,2 contact lens peripheral ulcer (CLPU),3 and infiltrative keratitis.4 Although rare, microbial keratitis (MK) is a sight-threatening contact lens–related infection.5–7 These continue to be an ongoing problem with contact lens wear for wearers and practitioners alike. A contact lens with high antimicrobial activity may inhibit microbial adhesion and consequently reduce these contact lens–related adverse events.
Antimicrobial peptides (AMPs) are small peptides and a part of the innate immune system of all multicellular organisms, with the native ability to inhibit microbial growth.8–13 Although more than 800 to 1000 AMPs have been discovered to date,14,15 only a few have been tested on animals and humans.16 Lipsky et al.17 evaluated pexiganan acetate cream to treat mildly infected diabetic foot ulcers in comparison with systemic ofloxacin and showed that the topical AMP cream was an effective alternative. Another phase III trial demonstrated that the use of omiganan was associated with significant reductions in catheter-related infections.18
Previous studies have shown that melimine, prepared by combining active regions of protamine (from salmon sperm) and melittin (from bee venom), is a broad-spectrum AMP.19,20 Covalently bound melimine on contact lenses has demonstrated high activity against a range of microorganisms, including fungi, Acanthamoeba, and various strains of multidrug-resistant bacteria.21 The coating is heat stable and not toxic to mammalian cells in vitro.19,21 In addition to its broad-spectrum antimicrobial activity,21 the coating was also capable of reducing the severity and incidence of CLPU and CLARE in animal studies.22 Thus, it would be worthwhile to investigate the in vivo biocompatibility of the broad-spectrum antimicrobial contact lens in a rabbit model following the guidelines of the International Organization for Standardization (ISO)23 and in a human clinical trial.
Covalent Attachment of Melimine to Contact Lenses
Melimine (T-L-I-S-W-I-K-N-K-R-K-Q-R-P-R-V-S-R-R-R-R-R-R-G-G-R-R-R-R) was synthesized by conventional solid-phase peptide synthesis by the American Peptide Company (CA, USA). Peptides with more than 80% purity were used in this study. Detailed procedures for covalent attachment of melimine onto contact lenses have been explained by Dutta et al.21 Briefly, etafilcon A lenses (base curve, 8.7 mm; diameter, 14.0 mm; Johnson & Johnson Vision Care Inc., Jacksonville, FL) were used for this study. Melimine was covalently bound to the contact lenses via EDC (1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride) coupling. Lenses were regularly washed with phosphate buffered saline (PBS) pH 7.4 (NaCl 8 g L−1, KCl 0.2 g L−1, Na2HPO4 1.15 g L−1, KH2PO4 0.2 g L−1) and incubated overnight with 10% wt/vol NaCl followed by soaking in PBS for 2 hours to remove any dissolved melimine remaining in the lens matrix. Subsequently, lenses were autoclaved (121°C) in PBS for 15 minutes. This covalent technique was able to attach 152 μg melimine onto contact lenses.21 Uncoated etafilcon A lenses were used as controls. Ten unused melimine-coated contact lenses were used as comparator to worn melimine-coated lenses during testing for retention of antimicrobial activity. To facilitate masking of the contact lens types during the trials, control lenses were carefully removed from the blister packets, washed three times in PBS, and autoclaved in 5 mL of PBS in a glass vial that is visually identical to the melimine contact lens vial. All the contact lenses were stored in a cold room (5°C) until required.
Animal Model for Assessing the Safety and Ocular Irritation of Melimine-Coated Lenses
This was a prospective, masked, randomized, controlled study conducted following the guidelines of ISO 9394.24 All animals were treated strictly in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research, and before the study commencement, approval from University of New South Wales Animal Care and Ethics Committee was obtained.
A total of six female New Zealand white rabbits were allocated for contralateral melimine and control contact lens wear. They were acclimated for at least 1 week. Only rabbits in good general health, weighing more than 3.5 kg, and having eyes free of clinically significant ocular irritation were used in the study. The nictitating membrane was not removed from the rabbits’ eyes. The test and control eyes were randomly assigned using Microsoft Office Excel (Microsoft, Redmond, WA).
Lens Wear and Assessments
Rabbits wore contact lenses for 7 to 8 hours daily for 21 consecutive days. Biotrue (Bausch & Lomb, Rochester, NY) multipurpose disinfecting solution was used for overnight storage, pre–lens insertion, and rinsing. Day 22 was the last day, and lenses were worn for at least 4 hours on this day. All rabbits were monitored daily for any indication of stress by examining their movements, alertness, gait, behavior, vocalizations, and respiration. Rabbits were weighed at baseline and days 8, 15, and 22 of contact lens wear. Special attention was given to observing any scratching or pawing of eyes, which might indicate ocular irritation. Detailed slit lamp ophthalmic examinations were performed at baseline and immediately after lens removal on days 8, 15, and 22 following the McDonald-Shadduck Score System24 by a masked observer. Conjunctival congestion/swelling/discharge, aqueous flare, iris involvement, corneal cloudiness, vascularization, and fluorescein staining were determined at each observation time. Baseline examinations were performed within 24 hours of starting the study. Slit lamp biomicroscopy (Nikon FS-3V, Tokyo, Japan) and detailed anterior segment examinations were carried out, including sodium fluorescein (Chauvin Pharmaceuticals Ltd, Surrey, United Kingdom) staining. Wratten no. 12 filter (Bausch & Lomb) was used in conjunction with cobalt blue filter to excite fluorescence. Contact lenses that fell out of the eye during the treatment period were thoroughly examined, rinsed, and reinserted. If the lenses were lost or damaged, new lenses were inserted as replacements. A maximum of four replacement lenses were allowed during the entire study. Lens retention on the rabbit eyes was checked frequently by visual inspection. After 8 hours of contact lens wear, the lenses were removed from each eye, inspected for damage, rinsed, and soaked overnight in the designated storage cases with solutions. On day 22, after the final ophthalmic observations, contact lenses were removed and all rabbits were euthanized.
Twelve corneas of six rabbits were collected in 4% formaldehyde (BDH Chemicals, Victoria, Australia) for histopathology. Corneal samples were placed in cassettes and then loaded into a Shandon Excelsior ES Tissue Processor (Thermo Fisher Scientific, Waltham, MA) for overnight processing (infiltration with paraffin). Samples were then removed and embedded in wax molds on a Shandon Histocentre 3 (Thermo Fisher Scientific, Pittsburgh, PA). Wax blocks were trimmed, and sections were cut on the Leica RM 2165 Microtome (Leica Microsystems, Rijswijk, The Netherlands) at 4-μm thickness. Slides were placed in a laboratory oven at 56°C for 1 hour and stained with hematoxylin and eosin using a Leica XL Autostainer (Leica Microsystems Inc., Bannockburn, IL). Slides were then coverslipped using the Dako CR 100 Coverslipper (Dako, Produktionsvej, Denmark) and allowed to dry overnight. Processed slides were stored at 4°C before microscopic examination.
Biocompatibility and Retention of Antimicrobial Activity in a Human Clinical Trial
A prospective, randomized, double-masked, contralateral, 1-day clinical trial was conducted using melimine-coated and control contact lenses. The participants’ comfort, dryness, and lens awareness with lenses and corneal health were evaluated, and the lenses were collected on completion of the study to determine the retention of antimicrobial activity.
Seventeen participants were enrolled to demonstrate a statistically significant difference in corneal staining score of 0.5 ± 0.7 at the 5% level of significance and 80% power. The study was approved by the Human Research Ethics Committee of the University of New South Wales and followed the tenets of the Declaration of Helsinki 1975, as amended in 2000, including local regulations as applicable such as Therapeutic Goods Administration, Australia. The clinical trial was conducted under the clinical trial notification scheme following the regulations of the Therapeutic Goods (Medical Devices) Regulations 2000. The clinical trial was registered in the publicly accessible Australian New Zealand Clinical Trial Registry (trial ID ACTRN 12613000369729).
Inclusion criteria required participants to be older than 18 years, in good health, not taking any medications, and correctable vision to 6/12 or better in each eye; both experienced and non–contact lens wearers were included in the study. Exclusion criteria were any preexisting ocular irritation; injury or condition (including infection or disease) of the cornea, conjunctiva, or eyelids that would preclude contact lens fitting and safe wearing of contact lenses; any systemic disease; eye surgery; systemic or topical medication up to 12 weeks before or during the trial that may adversely affect ocular health; and/or being pregnant or having had corneal refractive surgery.
Subjects were recruited from the subject population at Brien Holden Vision Institute and School of Optometry and Vision Science, University of New South Wales. Participants were screened for general clinical trial suitability by way of a routine eye examination that included refraction, visual acuity, and general eye health. Informed consent was obtained from all the participants before the trial.
Study Visits and Clinical Techniques
A baseline visit was conducted to assess the suitability of the participants, and baseline measurements were taken for the trial. A total of four visits were undertaken: lens dispensing (visit 1), lens collection after 8 hours (visit 2), and follow-ups after 1 and 4 weeks. Because both the follow-up visits included no assigned contact lens wear, participants were free to wear own lenses or glasses if needed. A follow-up visit after 4 weeks was conducted to rule out any delayed toxicity of melimine-coated lenses.
Visual acuity was measured at each visit using computer letter charts.25 Slit lamp biomicroscopy (Zeiss SL-120, Carl Zeiss Meditec, Jena, Germany) was performed by a single masked observer. All the clinical grading was conducted using the CCLRU26 grading scales (0 to 4 units) interpolated into 0.1 increments. Bulbar and limbal redness, palpebral redness and roughness, and corneal and conjunctival staining were assessed at all visits. Examination of corneal and conjunctival staining and lens-induced conjunctival indentation was conducted with fluorescein (Fluorets ophthalmic strips, 1 mg; Chauvin Pharmaceuticals Ltd) with the help of Wratten no. 12 filter (Bausch & Lomb, Rochester, NY) in conjunction with cobalt blue filter. Examination with fluorescein was conducted before and after contact lens wear in the lens dispensing and collection visit, respectively. Type, extent, and density of corneal staining were recorded in each of the five corneal zones according to the CCLRU staining criteria,26 as shown in Fig. 1. Fluorescein was carefully washed from the eyes completely before the lens insertion. Lenses were inserted and removed using aseptic gloves (DermaClean Sterile, Ansell Ltd, Richmond, Australia). Lens surface deposits and wetting, back surface debris, centration, tightness, fluting, primary gaze movement and gaze lag, corneal coverage, and overall acceptance were assessed at the lens dispensing and collection visits. Slit lamp photographs were taken using a Nikon photographic slit lamp (Nikon FS-3V; Nikon, Tokyo, Japan), which provided up to 32× magnification. Subjects were asked to rate the comfort of the lenses based on their overall impression of ocular comfort, ocular dryness, lens awareness, and lens edge awareness at the time of contact lens collection using a 1 to 10 scale using whole number steps (1 = very uncomfortable, dry, or aware; 10 = comfortable, not dry, or not aware). Participants were asked for the preference of either eye (forced preference: either right or left eye) based on contact lens wear experience. After wear, lenses were collected in glass vials containing 2 mL of sterile PBS.
Retention of Antimicrobial Activity
Worn and unworn contact lenses were processed for evaluation of retention of antimicrobial activity against Pseudomonas aeruginosa strain 6294 (isolated from a case of MK) and Staphylococcus aureus strain 31 (isolated from a case of CLPU) within 48 hours after a procedure detailed earlier.21 Briefly, bacteria were grown overnight in Tryptone Soya Broth (Oxoid, Basingstoke, United Kingdom) and then resuspended in 1/10 Tryptone Soya Broth (S. aureus) or PBS (P. aeruginosa) to an OD660nm of 0.1 (1.0 × 108 colony-forming units [CFUs] per milliliter). The bacterial cell suspensions were then serially diluted (1/10) to 1.0 × 106 CFU mL−1 for adhesion assays. Worn and unworn melimine-coated and uncoated contact lenses were transferred to 1 mL of bacterial suspensions in wells of 24-well tissue culture plates (CELESTAR, Greiner Bio-One, Frickenhausen, Germany). To allow bacterial adhesion, lenses were incubated for 18 hours at 37°C with shaking (120 rpm). Contact lenses were then washed three times with PBS to remove nonadherent cells and then stirred rapidly in 2 mL of PBS containing a small magnetic stirring bar. After log serial dilutions in Dey Engley neutralizing broth (DE; Becton, Dickson and Company, USA), 3 × 50 μL of each dilution were plated on a tryptic soy agar (Oxoid) containing Tween 80 and lecithin for recovery of cells. After 24 hours of incubation at 37°C, the viable bacteria were enumerated as CFUs per square millimeter. Results are expressed as the reduction in adherent viable bacteria. Triplicate measurements were performed on a minimum of three separate occasions.
Data were analyzed using Microsoft Office Excel and Statistical Package for the Social Sciences software for Windows software version 21.0 (SPSS, Inc., Chicago, IL). Percent of lens retention for rabbits was calculated as [(actual wear time for duration of study)/(total possible wear time for duration of study)] × 100. Analytical manipulation of the data, such as the sum or frequency of scores, was calculated where appropriate. Human clinical and subjective ratings were summarized using descriptive statistics. Differences between lens types were determined at each visit using paired t test or Wilcoxon signed rank test based on the type of variable. Frequency and percentage of participants preferring any of the contact lens types were reported for each preference category. The bacterial adhesion data were log10 (x+1) transformed before data analysis where x is the number of adherent bacteria in CFUs per square millimeter. Differences in bacterial adhesion were analyzed using independent two-sample t test. Statistical significance was set at 5%.
Melimine-Coated Contact Lens Wear in the Animal Model
All six animals included in the study maintained good health, and no abnormal behavior was observed during the study period indicative of ocular discomfort. Contact lens loss during the study was infrequent. Lens retention was 94% for melimine-coated contact lenses and 96% for control contact lenses.
Gross Ocular Examination
Gross ocular scores are presented in Table 1. Mild conjunctival redness (score 1) was observed twice with melimine lenses. Mild conjunctival discharge (score 1) and redness were observed once each with control contact lenses. The remaining eyes appeared normal (score 0) for both melimine and control lenses throughout the study.
Ophthalmic Observation by Slit lamp Biomicroscopy
Slit lamp biomicroscopy scores are presented in Table 2. Unless detailed in Table 2, all eyes appeared normal. Scores of “1” for fluorescent retention by the cornea are commonly noted in healthy rabbits’ eyes; thus, this score was not considered clinically significant. At baseline, corneal fluorescein staining (score 1) was observed in two eyes in each treatment group. Mild conjunctival congestion (score 1) and mild corneal fluorescein staining (score 1) were the only two other signs occasionally observed in the study in both the treatment groups.
Diffuse and fluorescent slit lamp photographs of melimine and control contact lenses worn by the same rabbit at baseline, day 8, day 15, and day 22 are shown in Fig. 2. The fluorescein photographs in this figure (pictures 1C to 4C and 1D to 4D) confirm the absence of corneal staining of control and test eyes. Neither melimine-coated nor control contact lens wear was associated with any other slit lamp biomicroscopy signs of ocular irritation, such as conjunctival chemosis or swelling, discharge, iris changes, corneal cloudiness, or vascularization. During the study period, observations made by slit lamp biomicroscopy indicated no significant clinical signs that might suggest ocular irritation induced by melimine coatings. None of the rabbits were discontinued from contact lens wear during the trial.
Histopathology of corneal sections stained with hematoxylin and eosin indicated no major structural differences between corneas exposed to melimine or control contact lenses (Fig. 3). All the sections from 12 corneas showed normal central and peripheral structures. All the three layers of corneal epithelium (basal layer, intermediate layer, and flattened cells) were intact and identical in all sections observed with high (40× objective) magnification.
Melimine-Coated Contact Lens Wear in Humans
A total of 17 participants were enrolled in this study, of which eight were experienced wearers. There were no disqualifications, and data from all the enrolled participants were included in the analysis. There were 10 females in this study, and the mean (±SD) age of the participants was 30.9 (±9.4) years. The mean (±SD) lens wearing time was 6.9 (±0.9) hours. Table 3 shows refractive error and keratometry readings at baseline visit (n = 34).
Clinical Signs and Symptoms
There were no significant differences seen in wettability or surface deposition between melimine-coated and control contact lenses during both lens dispensing and collection visits (p > 0.05). Melimine lenses showed clinically acceptable centration, movement, and tightness at all times. Overall fitting acceptance for both the lens types at both time points was rated highly (>3.0), which indicated complete corneal coverage, good centration, adequate primary gaze movement, and acceptable tightness. None of the contact lenses needed to be refitted, and no lens loss was reported.
There were no significant differences in different areas of bulbar redness, limbal redness, palpebral redness, and palpebral roughness between the melimine-coated and control lenses (p > 0.05). One participant with melimine-coated lenses showed slightly higher conjunctival staining in all four quadrants (mean difference in grade of 0.7). Overall, melimine lenses did not show any significant difference in conjunctival indentation and staining when compared with control lenses (p > 0.05). Melimine-coated contact lens wear was associated with significantly higher levels of corneal staining (Fig. 4) in all areas compared with the control lenses (p < 0.05; extent, depth, and type). Fig. 5 shows the extent, depth, and type (median; mean ± SD) of fluorescein staining associated with melimine and control lenses in all the corneal areas. Both corneal staining mean and median were higher in corneas with melimine lenses than controls (p < 0.05).
Overall, 65% participants preferred the control contact lenses. Distribution of comfort scores during melimine-coated and control contact lens wear is presented in Fig. 6 using box plots. One participant was uncomfortable with the melimine-coated lens and reported high levels of dryness, lens awareness, and lens edge awareness that are represented as the outliers in Fig. 6. Although there was no significant difference in overall comfort (p = 0.07), dryness (p = 0.10), lens awareness (p = 0.06), or lens edge awareness (p = 0.20), the mean responses were slightly lower with melimine-coated lenses. Standard deviations of comfort ratings for melimine lenses (range, 1.9 to 2.5) were higher than those for control lenses (range, 1.7 to 2.0).
Retention of Antimicrobial Activity
When incubated with P. aeruginosa 6294 and S. aureus 31, worn melimine contact lenses showed significantly lower adhesion (p < 0.05) when compared with worn control lenses, resulting in 1.5 ± 0.5 log and 1.5 ± 0.4 log inhibition in adhesion, respectively. Worn melimine lenses showed 0.5 ± 0.3 log (p = 0.05) and 0.8 ± 0.5 (p > 0.05) log higher P. aeruginosa 6294 and S. aureus 31 adhesion than unworn melimine lenses (Fig. 7). Pseudomonas aeruginosa 6294 and S. aureus 31 adhesion to contact lenses collected from each of the 17 participants is presented in Fig. 8.
This study provides the first evidence to indicate that AMP-coated contact lenses can be worn by humans without any major side effects. Although the contact lenses covalently coated with melimine showed increased human corneal staining, they retained antibacterial activity after 1 day of wear.
All the animals during this trial remained healthy and behaved normally, and no ocular irritation–related symptoms such as eye scratching or pawing of eyes were observed. Slit lamp and gross ocular observation of the cornea, conjunctiva, and ocular adnexa did not show any ocular signs that might indicate irritation. Corneal fluorescein staining indicated no difference between eyes during melimine or control contact lens wear. This was supported by the histopathological investigation that confirmed the absence of toxicity to corneal tissue, especially epithelium. A previous study has shown that melimine-coated contact lenses are able to reduce the clinical manifestations of CLPU and CLARE, arising from both gram-positive and gram-negative bacterial contaminations in rabbit and guinea pig models, respectively.22 Considering the results presented here and those of previously reported studies, it may be concluded that, at least in animal models, melimine-coated contact lenses are safe in the wear modalities that have been investigated and have the capacity to reduce the severity and or incidence of bacterially driven adverse events.
During the human trial, melimine-coated lenses performed similarly to the control lenses for lens surface characteristics, including wettability, deposition, and debris. This result is in agreement with the high in vitro hydrophilicity of melimine-coated lenses reported earlier.21 Similar to the antimicrobial fimbrolide-coated contact lenses,27 melimine lenses showed acceptable fit with optimum movement/tightness and centration. This finding is in agreement with our previous study determining that covalent immobilization of melimine does not change lens parameters in vitro.21
This study, for the first time, investigated biocompatibility of synthetic AMP in human eyes and is one of the few studies that have evaluated human responses of antimicrobial lens in a clinical trial.27–29 Melimine-coated lenses were not associated with conjunctival staining, bulbar and limbal redness, and palpebral redness and roughness. The melimine lenses were not associated with any delayed ocular toxicity. However, when compared with controls, melimine-coated lens wear was associated with significantly higher corneal fluorescein staining mean and median. Ten of 17 participants wearing melimine-coated lenses had clinically significantly (difference in corneal staining >0.5 grading) higher corneal staining. However, the time taken to resolve these stainings was not determined with an unscheduled visit, and participants were doing well after 1 week. The observed corneal staining was similar to that of solution-induced corneal staining (SICS) reported with the use of contact lens care solutions.30
Solution-induced corneal staining generally represents as diffuse corneal staining in at least four of the five regions.30 Similarly, fluorescein staining associated with melimine-coated lenses were greater in all the five corneal areas. Uptake of cationic biocides including polyhexamethylene biguanide, other quaternary ammonium compounds such as benzalkonium chloride, and polyquaternary ammonium compounds such as polyquaternium-131 has been strongly associated with the incidence of SICS.32–35 However, the exact mechanism of fluorescein interaction with corneal epithelial cells during SICS is not well understood.32,36–38 Fluorescein pooling,36,39 ionic interaction with negatively charged fluorescein,40 uptake by apoptotic cells,41 staining of dead/damaged cell contents with compromised membranes,36 and accumulations in the intercellular space on the ocular surface42 are various theories that have tried to explain this. However, Bandamwar41 has shown that accumulation of fluorescein solutions in the voids on ocular surface or in the intracellular space is unlikely to be the mechanism of corneal staining. Given that melimine is covalently coupled and not released from lenses, other hypotheses such as ionic interactions with cationic surfactants bound to epithelial cells and fluorescein molecules or adhesion of cationic compounds to cell membranes are unlikely to be applicable here. Fluorescein staining of dead cells is controversial, and a few studies have shown that dead cells were actually responsible for lowest staining intensities.41 In addition, Morgan et al.36 suggested that corneal staining cannot be explained by its uptake onto damaged epithelial cells. Apoptotic cells have demonstrated much higher fluorescein staining than live or dead cells.41 Perhaps the bound melimine might be inducing apoptosis in these cells. This effect was not reported earlier with the in vitro cytotoxicity assay.21 It should be noted however that the in vitro assay used mouse fibroblast cells and not human corneal epithelial cells. Interestingly, the fluorescein staining observed with melimine-coated lenses in human corneas was not detected in rabbit corneas. Rabbit tears have higher divalent cations such as Ca2+ and Mg2+ than those of humans.43 It appears that there may not be any ionic difference between rabbit tears and melimine-coated contact lens surface, whereas significant ionic difference with human tears may have stimulated corneal fluorescein staining.
Solution-induced corneal staining with the use of polyhexamethylene biguanide and polyquad-based multipurpose disinfecting solution has been associated with higher corneal infiltrative events.44 Whether melimine-coated lenses would be associated with inflammation because of the SICS-like response cannot be ruled out and needs further exploration. However, Szczotka-Flynn et al.45 showed that corneal staining is frequent during continuous contact lens wear and not associated with the development of corneal infiltrative events. This was a contradictory finding with the previous work by the same investigators46 and was a consequence of fluorescein staining grades being used in the earlier study46 that underreported corneal staining. Perhaps the incidence, mechanism, type, duration, and consequence of corneal staining with melimine-coated contact lenses should be minutely investigated in a larger clinical trial, especially considering the uncertainty to the causative mechanism behind corneal staining.
The median of comfort scores indicated that control lenses were associated with marginally higher comfort when compared with melimine-coated lenses. Mean grades of overall comfort scores, lens-related dryness, lens awareness, and lens edge awareness were also slightly higher with control lenses, but the differences with those for melimine-coated lenses were not detected to be clinically significant. This finding is in agreement with the finding that clinical relevance of SICS is not known and often not associated with patient symptoms.32,47,48 Comfort results of melimine-coated contact lens wear were consistent with the results with fimbrolide-coated antimicrobial contact lenses,27 showing slightly less comfort and increased dryness and lens edge and lens awareness. Although this study was not designed to evaluate statistical difference in participants’ preference, 65% of the participants preferred control lenses, indicating that 15% more participants (p = 0.22) felt better with control lenses than hypothesized (50%). It is difficult to correlate these subtle differences in comfort score, as the melimine covalent coupling procedure involved several additional laboratory steps that could have affected the comfort or preference responses.
Zhu et al.27 have shown that fimbrolide-coated antimicrobial contact lenses are safe in humans; however, they did not evaluate retention of antimicrobial activity. The current study showed that melimine-coated lenses retained 1.5 log (96.8%) inhibition against P. aeruginosa and S. aureus after contact lens wear. When compared with unworn melimine lenses, there was increased bacterial adhesion to worn melimine lenses, but the difference was not statistically significant. The opposite trend was seen with the control lenses, which showed 0.4 ± 0.2 (p > 0.05) log higher S. aureus adhesion compared with worn lenses, but that was not the case for P. aeruginosa. Contact lens wear has different effects on bacterial adhesion partly because of differences in tear components and microorganisms present in the ocular biota of wearers.49,50 Comparative ex vivo bacterial adhesion to worn and unworn etafilcon A lenses varies considerably between studies.51,52 Negatively charged methacrylic acid of etafilcon A lenses encourage S. aureus adhesion53 and deposition of the cationic protein lysozyme from tears.54–56 However, the attachment of the cationic peptide melimine is likely to result in an increased positive charge on the lens surface, perhaps making the surface either positive or neutral. The human tear film consists of various negatively charged components, such as phospholipid,57 mucins, and mucin-like proteins such as lubricin58 or the protein lipocalin,59,60 which may interact with the surface-bound melimine and perhaps may affect activity. This requires further investigation.
Susceptibility of AMPs to in vivo proteolytic degradation is possible and may limit the pharmacokinetics and functions of AMPs.61–63 These interactions may make AMPs unsuitable for certain applications. Trotti et al.64 investigated an AMP called “iseganan” in a mouthwash to reduce oral mucosis during radiotherapy treatment for head and neck cancer. The peptides failed to effectively reduce ulcerative events and subsequent morbidity possibly because the presence of various proteases and enzymes in the oral cavity may have reduced the activity of the AMP. An effective way to increase the stability of AMPs to degradation by proteolytic enzymes is to modify the C-terminus by amidation.65 Surface-attached melimine has been shown to retain activity after exposure to the proteolytic enzyme trypsin,66 indicating that this lens surface–immobilized melimine may be resistant to proteases at the ocular surface. The current study showed that melimine-coated contact lenses are active after 8 hours of human lens wear, indicating that melimine-coated contact lenses may have a permanent activity. Whether melimine could reduce contact lens–related adverse events during wear, especially extended wear, requires more extensive clinical trials. Given the incidence of CLARE, CLPU, and infiltrative keratitis, prospective clinical trials with melimine-coated lenses may be able to demonstrate a reduction. However, MK is relatively rare and postmarket studies may be required to demonstrate a reduction in incidence and severity.
In conclusion, this study has shown that melimine-coated contact lenses can be safely worn by humans without any major side effects. It is supported by animal study, and the antimicrobial benefit could be achieved without any adverse effect on mammalian eye health. Although melimine lenses were less preferred, subjective comfort scores were broadly comparable to those of uncoated control lenses. Melimine lens wear was associated with a higher corneal staining and retained antibacterial activity against P. aeruginosa and S. aureus after wear. Given the period of human contact lens wear, melimine lenses were biocompatible and retained antibacterial activity.
School of Optometry and Vision Science
Rupert Myers Bldg, Gate 14, Barker St
The University of New South Wales
Sydney New South Wales 2052
The authors thank Ms. Denise and Ms. Robyn Lawler from the University of New South Wales for assistance in the animal study, Dr. Thomas John for help with the statistical analysis, and Dr. Judith Flanagan for help with scientific writing.
This work is original, has not been published, and is not being considered for publication elsewhere. This work was partly presented as a paper at the American Academy of Optometry annual meeting 2013. There are no conflicts of interest for any of the authors that could have influenced the results of this work. The first author is supported by the University International Postgraduate Award and Maki Shiobara scholarship from the University of New South Wales, the Ezell fellowship (Bausch & Lomb) from the American Academy of Optometry, and the Brien Holden Vision Institute.
Received December 3, 2013; accepted January 23, 2014.
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Keywords:© 2014 American Academy of Optometry
peptide; contact lens; biocompatibility; melimine; antimicrobial activity