Secondary Logo

Journal Logo

Institutional members access full text with Ovid®

Method to Identify Demodex in the Eyelash Follicle Without Epilation

Mastrota, Katherine M.*

doi: 10.1097/OPX.0b013e318294c2c0

ABSTRACT The ubiquitous skin mite Demodex has been implicated as an instigator of anterior blepharitis. To date, overpopulation of the organism has been verified by cilia epilation (Coston or modified Coston method). This monograph describes an alternate technique to isolate Demodex organisms by eyelash rotation within the follicle. Via this method, without epilation, mites are made visible in follicles that may or may not display the cylindrical cuffing characteristic of demodicidosis.

Supplemental digital content is available in the text.


Omni Eye Surgery, New York, New York.

Katherine M. Mastrota Omni Eye Surgery New York 36 East 36th St New York, NY 10016 e-mail:

Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal’s Web site (

© 2013 American Academy of Optometry