Commonly, measurements of lens thickness are performed using A-scan ultrasonography or slitlamp Scheimpflug photography. Both techniques have their drawbacks in the study of presbyopia: ultrasonography requires the velocity of sound in the lens which may change with age, whereas Scheimpflug photography requires knowing the refractive index of the lens to enable correction of the photographs for the distortion due to the refraction of the cornea and lens. By combining Scheimpflug photography and axial optical eye-length measurements, we were able to individually correct the Scheimpflug images for distortion and calculate the refractive index and thickness of the human lens. Lens thickness of 90 subjects ranging in age between 16 and 65 years was measured, and an average increase of 24 μm/year was found. This value is consistent with ultrasonographic measurements assuming an age-independent velocity of sound in the lens of 1641 m/s. The posterior lens surface recedes from the cornea with age, and this backward movement does not differ significantly from the forward movement of the anterior lens surface.