GYNECOLOGY: INFERTILITY/REPRODUCTIVE TECHNOLOGIESExpanding Homogeneous Culture of Human Primordial Germ Cell–Like Cells Maintaining Germline Features Without Serum or Feeder LayersKobayashi, Mutsumi; Kobayashi, Misato; Odajima, Junko; Shioda, Keiko; Hwang, Young Sun; Sasaki, Kotaro; Chatterjee, Pranam; Kramme, Christian; Kohman, Richie E.; Church, George M.; Loehr, Amanda R.; Weiss, Robert S.; Jüppner, Harald; Gell, Joanna J.; Lau, Ching C.; Shioda, Toshi Author Information Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA (Mutsumi Kobayashi, Misato Kobayashi, J.O., K. Shioda, T.S.); Institute for Regenerative Medicine, Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA (Y.S.H., K. Sasaki); MIT Media Lab, Massachusetts Institute of Technology, Cambridge (P.C.); Department of Genetics, Harvard Medical School (P.C., C.K., R.E.K., G.M.C.); Wyss Institute for Biologically Inspired Engineering, Harvard University (P.C., C.K., R.E.K., G.M.C.), Boston, MA; Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY (A.R.L., R.S.W.); Endocrine Unit and Pediatric Nephrology Unit, Massachusetts General Hospital and Harvard Medical School, Boston, MA (H.J.); The Jackson Laboratory for Genomic Medicine, Farmington (J.J.G., C.C.L.); Connecticut Children’s Medical Center, Hartford (J.J.G., C.C.L., T.S.); and University of Connecticut School of Medicine, Farmington (J.J.G., C.C.L.), CT Obstetrical & Gynecological Survey: May 2022 - Volume 77 - Issue 5 - p 280-281 doi: 10.1097/01.ogx.0000827980.67713.d1 Buy Metrics Abstract (Abstracted from Stem Cell Rep 2022;17(3):507–521) Primordial germ cells (PGCs) are embryonic precursors of gametes that are extensively studied in research settings. Human primordial PGC–like cells (hPGCLCs) are a surrogate model for embryonic PGCs that present difficulties in in vitro cell culture and expansion due to rapid loss of PGC-like identity and limited cell survival. Copyright © 2022 Wolters Kluwer Health, Inc. All rights reserved.