(Abstracted from Prenatal Diagn 2018;38(10):765–771)
Noninvasive prenatal testing (NIPT) analyzes cell-free DNA (cfDNA) in maternal plasma, which is a combination of DNA from maternal cells and from placental cytotrophoblast. The technique of massively parallel sequencing (MPS) sequences and counts large numbers of unique, single-locus DNA fragments and assigns them to the chromosome of origin.
Sydney Ultrasound for Women, Bondi Junction (F.S., R.S., M.-E.S., A.M.); University of New South Wales, Kensington (F.S.); Genea, Sydney (M.B., K.E.); and Discipline of Obstetrics, Gynaecology and Neonatology, University of Sydney, Camperdown (A.M.), New South Wales, Australia