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Measurement of plasma volume using99 Tcm-labelled DMP-HSA

DU RAAN, H.1,*; LÖTTER, M. G.1; HARRIS, G. W.1; VAN ASWEGEN, A.1; OTTO, A. C.2; NEL, M. G.2; BADENHORST, P. N.3; VERBEKE, K. A.4; VERBRUGGEN, A. M.4

Nuclear Medicine Communications: June 2000 - Volume 21 - Issue 6 - p 539-544
Review Article
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Red cell volume (RCV) and plasma volume (PV) measurements are performed routinely in nuclear medicine departments to diagnose a number of haematological disorders. Currently, 125I-HSA is used as a plasma tracer and 99Tcm-labelled red cells to determine red cell volume. 125I-HSA is not always readily available, leading to inconvenience for patients and medical practitioners. Due to the availability of 99Tcm in nuclear medicine departments, the use of albumin labelled with 99Tcm was investigated. A new 99Tcm-human serum albumin labelling kit (99Tcm-DMP-HSA) was developed by Verbeke and supplied for use in this study. The main aim of the study was to investigate the use of 99Tcm-DMP-HSA for PV determination. Secondly, the feasibility to determine red cell and plasma volume simultaneously using 99Tcm as radionuclide in both instances was investigated. Fourteen healthy volunteers were enrolled in the dual-phase study. During the first study, 99Tcm-DMP-HSA was used as tracer to calculate PV (PV1a) after intravenous administration. Subsequently, 99Tcm-labelled red cells were administered and the PV (PV1b) and RCV (RCV1) were calculated. The second study was repeated within 2 weeks using the conventional method. 125I-HSA and 99Tcm-labelled red cells were administered simultaneously. The PV (PV2) and RCV (RCV2) were calculated. We found that the redistribution of 99Tcm-DMP-HSA is faster than that of 125I-HSA; therefore, the plasma counts obtained at different times were back-extrapolated to time zero for plasma volume calculations. The mean values for the different calculated PVs were 2964±470 ml for PV1a, 3006±623 ml for PV1b and 3001±530 ml for PV2, the reference PV. The confidence intervals indicate no significant differences between plasma volumes PV1a and PV2 and plasma volumes PV1a and PV1b. The mean calculated RCV1 was 2130±322 ml; that of RCV2 was 2128±353 ml. The difference between RCV1 and RCV2 was not significant. Our results indicate that 99Tcm-DMP-HSA could be used for plasma volume calculation. Red cell and plasma volumes can be calculated simultaneously using 99Tcm as radionuclide in both cases.

Departments of 1Medical Physics, 2Nuclear Medicine and 3Haematology, University of the Orange Free State, Bloemfontein, South Africa and 4Laboratory of Radiopharmaceutical Chemistry, Catholic University Leuven, Leuven, Belgium

*Address all correspondence to Hanlie du Raan, Department of Medical Physics, University of the Orange Free State, PO Box 339, Bloemfontein 9300, South Africa. e-mail: gnbihn@med.uovs.ac.za

Received 26 January 1999 and accepted in revised form 21 December 1999

© 2000 Lippincott Williams & Wilkins, Inc.