Twenty-one healthy, physically active but not highly trained, college-aged men volunteered to take part in the experiment. The participants were fully informed of the risks and stresses associated with the study and gave written informed consent to participate in this study as approved by the Medical Research Ethics Committee of the Academy of Physical Education and Sport. Each participant underwent preliminary exercise testing to become familiar with the exercise model and to obtain a measure of their maximal aerobic work capacity 1 week before the experimental trial. The participants were randomly assigned to either the interval training (IT; n = 10) or control (C; n = 11) group.
The first stage of the study (baseline measurements and first 3 weeks of training) was conducted during the semester break. The last 3 weeks of training was performed during the first 3 weeks of the semester. Nevertheless, the 6 weeks of the protocol were contiguous. The participants' class schedules included both theoretical and physical activity units, respectively, 23 and 7 h wk−1.
During the study, neither group reported participating in any organized or recreational physical activity outside the protocol; only the IT group performed the interval training. The physical activity classes in which the participants were enrolled were designed with a primary focus on the methods of teaching physical education, not for promoting fitness. All participants, according to self-reports, strictly adhered to the study's physical activity restrictions, thereby increasing the likelihood that the changes observed resulted from the interval training instead of other outside influences.
Body mass (BM) and body composition were estimated using a bioelectrical impedance floor scale (TBF-300 Body Fat Monitor/Scale Analyzer, Tanita, Japan) calibrated in accordance with manufacturer guidelines before each test session. One hour after a light breakfast, participants voided their bladder and bowels and, clad only in briefs, underwent duplicate measures while in the standing position recommended by the manufacturer guidelines. The average of the 2 values was used for final analysis.
Aerobic Power Measurement
To determine V̇o2max and anaerobic threshold (AT), participants performed a graded cycle ergometry test on an electromagnetically braked cycle ergometer (ER 900 Jaeger, Germany/Viasys Health Care). The ergometer seat height was individually adjusted to attain a 5° bend in the knee at the lowest point in the pedal revolution. Participants were allowed a 5-minute warm-up period at an intensity of 1.5 W·kg−1 with a pedaling cadence of 60 rpm. Immediately after the warm-up, the participants began V̇o2max testing by cycling at increasingly difficult workloads in which resistance was increased by 25 W·min−1 until the participant reached the point of volitional exhaustion. The recovery was passive with the participant in a seated position. Breath-by-breath pulmonary gas exchange was measured (Oxycon-Pro, Jaeger-Viasys Health Care, Hochberg, Germany) throughout the V̇o2max test; the O2 and CO2 analyzers were calibrated before each test using standard gases of known concentrations in accordance with manufacturer guidelines. The nonlinear increase in ventilation (ventilatory threshold) was used to determine AT. Heart rates were monitored continuously by telemetry (S-625, Polar Electro-Oy, Finland) during each test session and the first 5 minutes of passive recovery in a seated position.
Anaerobic Power Measurement
Participants performed the WAnT on a mechanically braked cycle ergometer (884E Sprint Bike, Monark, Sweden) according to the procedures described previously (2,23). The testing session started with a standardized 5-minute warm-up cycling at 1.0 W·kg−1·BM−1 including 2 all-out sprints lasting 3-5 seconds each against the resistance used for the actual test. After a 5-minute rest, the WAnT began from a stationary starting position with the participant seated and the right pedal at approximately 45° as previously described (23,30). The participants were instructed to accelerate to their maximal pedaling rate and were verbally encouraged to maintain this pedaling cadence as long as possible throughout the 30-second test. Only during the first few, initial movements of the test, was each participant able to pedal in a standing position; this helped overcome the resistance and achieve the maximal pedaling rate. The remaining part of the test was performed with the participant in a seated position. A flywheel resistance equaling 0.075 kg·kg·BM−1 (corresponding to 7.5% of each individual's BM) was applied at the onset of the WAnT (10). After test termination, the participants were supervised during a 15-minute recovery in a seated or supine position. All tests were performed at similar times in the morning at least 2 hours after a light breakfast.
The computer software automatically calculated mechanical power by multiplying braking force by velocity (pedaling cadence) for each 5-second segment of the WAnT. Four other indices describing the participant's WAnT performance were also calculated programmatically. Peak power (PP), defined as the highest mechanical power achieved at any stage of the test, typically occurred in the first few seconds of the WAnT and was used to represent the explosive characteristics of the participant's muscle power. Mean power (MP), the average power sustained throughout the 30-second period, was used to represent the average local muscle endurance throughout the WAnT. The fatigue index (FI) or degree of power drop from PP to the lowest power at the end of the test, and was expressed in W·s−1, W·kg·BM−1 and as a percentage of PP. Total work (Wtot) was used to describe muscle endurance and was calculated by multiplying MP by 30 seconds (duration of the WAnT protocol) and expressed in Joules (J) or J kg·BM−1 (2). It was assumed that PP primarily reflected the participant's ability to convert alactic (phosphagenic) energy, whereas MP mainly reflected the combined rate of lactic (glycolytic-nonphosphagenic), phosphagenic, and aerobic energy conversion.
In the present study, the phosphagenic and nonphosphagenic components of work were calculated as previously described (38). This mathematical method is based on calculating the total area under the curve from the beginning to the end of WAnT (from 0 to 30 seconds on the horizontal axis). The total diagram area (Figure 1) is subsequently separated into 2 components, divided at the perpendicular line from point A on the power course curve to point B on the time axis; point B corresponds to the onset of power drop from PP (point A). The first of the 2 parts corresponds to the phosphagenic (Wanaphosph) component of work, and the second one represents the nonphosphagenic (Wananonphosph) component of work. This method provides an estimation of the proportion of Wanaphosph and Wananonphosph contribution to the total anaerobic energy turnover during the WAnT. Wanaphosph and Wananonphosph may be expressed as percentages of total anaerobic work (Wanatot).
Blood Lactate Analysis
Blood samples were collected from an antecubital vein as part of the baseline and follow-up WAnT procedures with samples taken before warm-up and 5 and 15 minutes after WAnT. Immediately after collection, the blood was deproteinized by the addition of ice cold 0.4 M perchloric acid. After being thoroughly mixed, the samples were centrifuged at 12,000g for 10 minutes. Blood LA was determined using a standard Randox (Crumlin, United Kingdom) kit based on the LA oxidase method (LC2389); assays were performed on a Cecil CE9200 spectrophotometer (Cambridge, United Kingdom).
Participants in C continued with their regularly scheduled classes and activities; Participants in IT, also in the same academic program, continued with these same regularly scheduled activities in addition to the training protocol. The IT group met every Monday, Wednesday, and Friday for 6 weeks. Each training session began at 0900, 1 hour after the participants ate a light breakfast. The same research assistant who controlled the flywheel resistance and also timed the interval and recovery periods supervised all sessions. The ergometer (884E Sprint Bike, Monark, Sweden) seat height was individually identified for each participant (5° bend in the knee at the lowest point in the revolution) and maintained throughout the intervention period. Each training session began with a 5-minute warm-up at an intensity approximating 30% of maximal aerobic power (30% p V̇o2max). Six 90-second cycling bouts at 80% p V̇o2max were then performed at a cadence of 60 rpm. Each 90-second bout was followed by 180 seconds of passive rest for a work-to-rest ratio of 1:2. All laboratory sessions were performed in ambient conditions of 20-22°C and 60% humidity.
Statistical analyses were performed using Statistica 8.0 for Windows. A 2 (group) × 2 (time) repeated-measures analysis of variance was used to investigate the significance of differences between groups and pre and posttraining sessions. The normality of data was tested using the Shapiro-Wilks W test. To assess the influence of initial values on power output, Pearson correlations were performed to compare the relationship between the pre and postintervention values of power at V̇o2max in absolute and relative terms. Statistical significance was set at p ≤ 0.05 for all analyses. Targeting statistical power at β = 0.8 with an effect size of 0.7, a sample size estimate of 12 participants per group was determined (39).
All participants completed the study, and no adverse events were reported. The Shapiro-Wilks W test was significant (p < 0.05). At baseline, there were no significant differences in basic anthropometric characteristics between groups (Table 2) or in aerobic (Table 3) and anaerobic performance (Table 4). There were significant (p < 0.05) main effects of group and time for several aerobic (Table 3) and anaerobic parameters (Table 4). Additionally, as shown in Table 5, there was a significant main effect of group for LA at the 5 and 15 minutes post-WAnT (mmol·L−1) marks for IT; p < 0.05. Significant interaction effects were noted for absolute (L·min−1) and relative (ml·kg−1·min−1) V̇o2max, absolute (W) and relative (W·kg−1) power at V̇o2max, power at AT, V̇o2 at AT (ml·kg−1·min−1), work output (J·kg−1), and MP (W·kg−1) with those in IT, but not C, experiencing improvements; p < 0.05 (Tables 3 and 4).
The 6-week HIIT program favorably influenced the aerobic and anaerobic performances for the IT group. Absolute and relative V̇o2max increased (p < 0.05) by an average of 0.5 L·min−1 (13%) and 5.5 ml·kg−1·min-1 (11%), respectively (Table 3). The pre and posttraining correlations between aerobic performance expressed in absolute (r = 0.93) and relative (r = 0.86; Figure 2) power terms for IT indicate that their training effect was similar regardless of their respective initial values. Improvements (p < 0.05) of 29.5 W and 0.29 W·kg−1 in absolute and relative power at AT, respectively, and 3.8 ml·kg−1·min−1 in V̇o2max at AT were recorded for IT on average (Table 3). Mean values for absolute and relative power at AT decreased slightly for C between the pre and posttraining periods.
As indicated by data presented in Table 4, the IT group improved in several anaerobic performance variables when compared to C. Work output (J·kg−1) increased 4.3% for IT but only by 1.4% for C. Although the average nonphosphagenic component of work (J·kg−1) increased for both groups, the change was larger for IT (12.5 J·kg−1; 6.8%); this was also reflected through an increase in the mean difference of the relative contribution of nonphosphagenic component of work to the total amount of work performed (1.6%) and a corresponding decrease of 1.7% in the relative contribution of the phosphagenic system for IT. Only in IT group, was a significant correlation between V̇o2max and nonphosphagenic component of total work (%) observed (Figure 3). The relative contribution of the anaerobic energy sources changed by less than 0.5% for C. Mean power improved, on average, for IT only. Both groups attained PP more quickly at the end of the 6-week period; however, the decrease in time to attain PP was 0.9 seconds for IT compared to 0.5 seconds for C. On the other hand, the mean difference in the ability to sustain PP revealed that C was able to maintain PP production 0.4 seconds longer than was IT during follow-up testing. Both groups demonstrated that LA values increased from resting values to 5 minutes postexertion with a decline after an additional 10 minutes of rest (Table 5). Significant (p < 0.05) improvements in LA response were noted only for IT. Compared to baseline, LA values for IT when 5 and 15 minutes into recovery were 3.1 and 2.0 mmol·L−1 lower, respectively, after HIIT training.
The major finding of this study was that 27 min·wk−1 of HIIT performed on a stationary cycle ergometer at a 1:2 work-to-rest ratio throughout a 6-week period significantly improved numerous variables associated with aerobic and anaerobic performance but decreased LA formation. Our protocol requiring the participants to cycle at 80% of their baseline p V̇o2max with a 1:2 work-to-rest ratio was tolerated by all in the IT group.
Unlike in the protocol used by Laursen et al. (27) in which they adjusted the training workload after a midpoint reassessment, our workload remained constant over the 6-week intervention. We observed an 11% increase in V̇o2max (ml·kg−1·min−1) for the IT group, whereas Laursen et al. (27) reported an increase of 6%. The 5% difference in final outcome between the 2 studies may be the result of the initial cardiorespiratory training levels of the participants and the duration of the interventions. There are several other studies showing increases in the maximal aerobic capacity (V̇o2max) after interval training (16,29).
Our results also support the findings of Creer et al. (16) and MacDougall et al. (29) even though these 2 studies used a 1:8 work-to-rest ratio for a sample of trained cyclists and healthy college students, respectively. Although we noted an average improvement in V̇o2max of 5.5 ml·kg−1·min−1 after a 6-week protocol using a 1:2 work-to-rest ratio, MacDougall et al. (29) reported an improvement of 3.5 ml·kg−1·min−1 after a 7-week protocol. Creer et al. (16) assigned their trained male cyclists into endurance-only and short-sprint-plus-endurance intervention groups; they noted an increase in V̇o2max of 0.2 L·min−1 for both groups. As shown in Table 3, we reported an increase of 0.41 L·min−1 for the IT group. Again, the initial conditioning level of the participants compared to ours cannot be overlooked when comparing the results from these 2 studies. In terms of indicators of aerobic endurance, those in the IT group of the present study increased their V̇o2 consumption at AT by 3.5 ml·kg−1·min−1; however, AT as a percentage of V̇o2max at posttesting did not differ from the baseline values. Similarly, Creer et al. (16) reported no change in the percentage of maximal heart rate at which ventilatory threshold was observed.
To investigate the effects of HITT on anaerobic capacity, a Wingate test was performed. We noted that total work output was significantly higher in the IT group. As performance in WAnT is dependent on phosphagenic, glycolytic and, partially, oxidative metabolism, these data indicate that at least one of these energetic systems had improved after interval training. Our data indicate that the phosphagenic work was the same in both groups but the nonphosphagenic component of work tended to be higher in the IT group. Recent evidence suggests that during exercise lasting up to 30 seconds, a substantial amount of energy is derived from aerobic metabolism. Therefore, we consider that improvement in WAnT could be a result of increase in glycolysis and oxidative phosphorylation. To evaluate the influence of HIIT on glycolysis, we measured the blood LA concentration after WAnT. Blood LA values taken after the WAnT were lower on average for our IT group (Table 5). This 3-mmol·L−1 decrease in concentration at the 5-minute mark indicates that LA formation was significantly lower in the IT group. On the other hand, when we compared changes in LA concentration 15 minutes after WAnT, there was no difference between groups. These data suggest that 6 weeks of HIIT did not influence the rate of LA removal.
Our data are in agreement with those of Burgomaster et al. (11); their sprint cycling group had lower rates of glycogenolysis and, hence, muscle LA accumulation because the men in that group also had an increased ability to oxidize pyruvate. Moreover, 8 weeks of endurance training was sufficient to increase nicotinamide adenine dinucleotide reduced form (NADH) shuttle enzymes levels by approximately 50%; this enzymatic system competes with LA dehydrogenase for NADH (35). Because we had no capabilities of measuring NADH shuttle enzyme activity, we can only speculate that our training protocol decreased LA formation by increasing oxidative capabilities of cytoplasmic NADH via the shuttle enzymes. Indeed, recent evidence suggests that 13% of energy during a 10-second sprint and 27% of energy during a 20-second sprint is generated aerobically (9,24). Postintervention results for the IT group in the present study revealed an increased nonphosphagenic component of total work performed during WAnT (Figure 3). All together these data indicate that an increase in total work output during WAnT is a result of an increase in aerobic metabolism. This conclusion is in direct opposition to the studies reporting an increase in anaerobic metabolism as a result of HIIT. For example, Creer et al. (16) noted higher LA levels for the short-sprint-plus-endurance training group during posttraining compared to baseline for each of the 4 sprints and at 3- and 6-minutes into recovery after the last sprint. Using a sample of college students similar to those in the present study, MacDougall et al. (29) noted increases in oxidative and glycolytic enzymes after a 7-week intervention of 30-second sprint sessions 3 times per week. Our data indicate that 6 weeks of an HIIT protocol with a 1:2 work-to-rest ratio is an effective means to improve aerobic capacity and that the increased performance during WAnT is a result of increased aerobic capacity.
For coaches, athletes, and fitness practitioners who need to quickly improve maximal aerobic and anaerobic cycling performance, using our 1:2 work-to-rest HIIT protocol should produce notable and favorable changes with minimal time invested in cycling-specific training per week. This protocol may shorten preseason training requirements or provide a time-efficient method to maintain in- and postseason performance capacities of men similar to those in our study. For the tennis players who lost 4-15% of their aerobic capacity during a 3-6 week break (31) and for other athletes, our HIIT protocol with a 1:2 work-to-rest ratio may be a time-efficient means to counter detraining during the off season.
Gratitude is expressed to all the participants involved in this study. This project was supported by the Committee of Scientific Research (KBN), and a grant from the Academy of Physical Education and Sport funded this project in its entirety. There are no conflicts of interest to be reported by the authors, because there are no professional relationships with companies or manufacturers who will benefit from the results of the present study. The results presented herein do not constitute an endorsement of the protocol by the NSCA nor by the authors as the only method by which to quickly show such improvements in aerobic and anaerobic performance. Disclosure of funding: There was no external funding for this project.
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Keywords:Copyright © 2011 by the National Strength & Conditioning Association.
blood lactate; Wingate; Training adaptations; cycling