Bishop, C, Abbott, W, Brashill, C, Loturco, I, Beato, M, and Turner, A. Seasonal variation of physical performance, bilateral deficit, and interlimb asymmetry in elite academy soccer players: Which metrics are sensitive to change? J Strength Cond Res XX(X): 000–000, 2022—This study aimed to report seasonal variations for (a) physical performance, (b) interlimb asymmetry, and (c) bilateral deficit (BLD) data over the course of a competitive soccer season and determine which metrics are sensitive to change. This study used a repeated-measures observational design for 19 under-20, elite, academy, soccer players (age: 17.58 ± 0.61 years; height: 1.81 ± 0.09 m; body mass: 74.36 ± 7.58 kg), who conducted bilateral and single-leg countermovement jumps (CMJ and SLCMJ), linear speed (5, 10, 20, and 30-m) and 505 change of direction (COD) speed tests, at pre, mid, and end of season time points. For jump tests, jump height, reactive strength index modified, time to takeoff, and countermovement depth metrics were monitored, with interlimb asymmetry and the BLD also calculated for each. Significant improvements (p < 0.05) in performance were evident in all fitness tests: CMJ (effect size [ES]: 0.61 to 1.03), SLCMJ (ES: 0.60 to 2.25), linear speed (ES: −0.54 to −1.96), and COD speed (ES: −0.68 to −1.14). Significant reductions in asymmetry (ES: −0.68 to −1.07) and significant increases in the BLD (ES: 1.15 to 1.57) were also evident throughout the season. Additionally, kappa coefficients were used to determine consistency in limb dominance throughout the season, but only poor to fair levels of agreement (kappa: −0.17 to 0.37) were evident, highlighting the fluctuating nature of limb dominance throughout the season. Despite all tests exhibiting meaningful change, the SLCMJ and linear speed showed the most frequent and largest differences in performance, highlighting their usefulness in the ongoing monitoring process of physical capacities in elite male academy players across a competitive soccer season.