Marins, EF, Cabistany, L, Bartel, C, Dawes, J, and Del Vecchio, FB. Effects of personal protective equipment on the performance of Federal Highway Policemen in physical fitness tests. J Strength Cond Res 34(1): 11–19, 2020—Personal protective equipment (PPE), worn by police officers, provides protection and can modify physiological and performance responses during physical efforts. Physiological, perceptual, and physical responses were compared with PPE (WPPE) and without PPE (NPPE) among the Brazilian Federal Highway Policemen (FHP). Nineteen (n = 19) FHP completed 2 experimental trials: NPPE and WPPE (load = 8.3 kg). Tests of aerobic and anaerobic power, muscular strength and endurance, as well as change of direction speed (CODS) were performed under both conditions. Heart rate reductions were shown at the second ventilatory threshold (1.4%) and maximal exercise (1.5%) WPPE. In comparison with NPPE, physical performance in the WPPE condition showed decreases in the following: treadmill time at maximal exercise (21%, p < 0.001); time in the isometric trunk test (28.9%, p < 0.001); vertical jump height (11.6 and 10.5%, p < 0.001); standing long jump distance (7.3%, p < 0.001); bar hang time (14.8%, p < 0.05); and CODS (2.6%, p < 0.05). Results indicate that PPE use reduces physical performance of FHP in cardiorespiratory, strength, power, and CODS tests.