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Effects of Creatine Supplementation During Resistance Training on Myosin Heavy Chain (MHC) Expression in Rat Skeletal Muscle Fibers

Aguiar, Andreo F1; Aguiar, Danilo H2; Felisberto, Alan DS1; Carani, Fernanda R1; Milanezi, Rachel C1; Padovani, Carlos R3; Dal-Pai-Silva, Maeli1

The Journal of Strength & Conditioning Research: January 2010 - Volume 24 - Issue 1 - p 88-96
doi: 10.1519/JSC.0b013e3181aeb103
Original Research

Aguiar, AF, Aguiar, DH, Felisberto, ADS, Carani, FR, Milanezi, RC, Padovani, CR, and Dal-Pai-Silva, M. Effects of creatine supplementation during resistance training on mysoin heavy chain (MHC) expression in rat skeletal muscle fibers. J Strength Cond Res 24(1): 88-96, 2010-The purpose of this study was to utilize a rodent model to test the hypothesis that creatine (Cr) supplementation during resistance training would influence the pattern of slow-twitch muscle myosin heavy chain (MHC) isoforms expression. Male Wistar rats (2-3 months old, 250-300 g) were divided into 4 groups: Nontrained without creatine supplementation (CO), nontrained with creatine supplementation (CR), trained without creatine supplementation (TR), and trained with creatine supplementation (TRCR). TR and TRCR groups were submitted to a resistance training program for 5 weeks (5 days/week) for morphological and biochemical analysis of the soleus muscle. Weightlifting exercise involved jump sessions into water, carrying progressive overload equivalent to percentage of body weight. CR and TRCR groups were given creatine at 0.5 g/kg−1/d−1. Both Cr supplementation and resistance training alone or associated did not result in significant alterations (p > 0.05) in body weight gain, food intake, and muscle weight in the CR, TR and TRCR groups compared to the CO group. Also compared to the CO group, the CR group showed a significant (p < 0.02) increase in MHCI content and a reduction in MHCII; inversely, the TR group increased the MHCII content and reduced MHCI (p < 0.02). When combined, both creatine and resistance training did not promote significant (p > 0.05) changes in MHC content of the TRCR group compared to the CO group. The data show that Cr supplementation provides a potential action to abolish the exercise-induced MHC isoform transitions from slow to fast in slow-twitch muscle. Thus, Cr supplementation might be a suitable strategy to maintaining a slow phenotype in slow muscle during resistance training, which may be favorable to maintenance of muscle oxidative capacity of endurance athletes.

1UNESP, Morphology Department, Botucatu, São Paulo, Brazil; 2UEPB, Biology Department, João Pessoa, Paraíba, Brazil; and 3UNESP, Bioestatistic Department, Botucatu, São Paulo, Brazil

Address correspondence to Andreo Fernando Aguiar,

© 2010 National Strength and Conditioning Association