Original ArticlesInterleukins and Ischemic Stroke: Effect of Neutralization of Rat IL-6 Bioactivity on the Degree of Ischemic Cerebral Damage Caused by Experimental Middle Cerebral Artery Occlusion in the RatTuna, Metin MD; Erman, Tahsin MD; Ylmaz, Derviş M. MDıAuthor Information Department of Neurosurgery, Çukurova University School of Medicine, Adana, Turkey Supported by Grant no TF9841 from The Research Fund of Çukurova University, which was awarded to Dr Tuna. This study was approved by Ethical Committee of Medical Faculty and carried out in The Research Center of Medical Sciences of Çukurova University. Reprints: Metin Tuna, MD, Department of Neurosurgery, Çukurova University School of Medicine, Balcalı, Adana 01330, Turkey (e-mail: email@example.com). Neurosurgery Quarterly: December 2008 - Volume 18 - Issue 4 - p 256-260 doi: 10.1097/WNQ.0b013e3181732ba8 Buy Metrics Abstract The purpose was to investigate the effect of neutralization of rat interleukin-6 (IL-6) bioactivity on the activity levels of Na+-K+/Mg++ adenosine-5′-triphosphatase and superoxide dismutase, and the degree of infarct size in ischemic brain produced by experimental middle cerebral artery occlusion in the rat. Seventy adult male Wistar rats weighing 280 to 310 g were used in this study. The animals were randomly allocated to groups: group I (n=10) consisted of normal controls, group II (sham-operated, n=20) underwent surgical exposure of the middle cerebral artery but no cauterization, and the remaining 40 rats were subjected to middle cerebral artery occlusion. Immediately after occlusion, each of these 40 rats was randomly assigned to either the occlusion-only group (group III, n=20) or the occlusion plus IL-6 antibody treatment group (group IV, n=20). All evaluations were performed in blinded fashion. Our data provide evidence that neutralization of IL-6 bioactivity correlates with less secondary damage in long-term treatment after focal cerebral ischemia in the rat. © 2008 Lippincott Williams & Wilkins, Inc.