CELLULAR, MOLECULAR AND DEVELOPMENTAL NEUROSCIENCEAmyloid precursor protein binding protein-1 knockdown reduces neuronal differentiation in fetal neural stem cellsHong, Bo-Hyuna; Ha, Sungjia; Joo, Yuyounga; Kim, Jeong A.a; Chang, Keun-A.a; Woo, Ran-Sookb; Suh, Yoo-Huna; Kim, Joung-Hunc; Kim, Hye-Suna,d Author Information aDepartment of Pharmacology, Seoul National University, College of Medicine, Seoul bDepartment of Anatomy, Eulji University, College of Medicine, Daejeon cDepartment of Life Science, Postech, Pohang, Kyungbuk dSeoul National University Bundang Hospital, Seoul National University College of Medicine, Bundang-Gu, Sungnam, Korea Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Website (www.neuroreport.com). Bo-Hyun Hong and Sungji Ha contributed equally to this study. Correspondence to Dr Hye-Sun Kim, PhD, Department of Pharmacology, College of Medicine, Seoul National University, Seoul, 110-799, South Korea Tel: +82 2 740 8298; fax: +82 2 741 8298; e-mail: [email protected] Received August 26, 2011 Accepted October 23, 2011 NeuroReport: January 25, 2012 - Volume 23 - Issue 2 - p 61-66 doi: 10.1097/WNR.0b013e32834e7d4f Buy SDC Metrics Abstract Amyloid precursor protein binding protein-1 (APP-BP1) was first identified as an interacting protein of APP. In this study, we explored whether APP-BP1 plays a role in neuronal differentiation of fetal neural stem cells. APP-BP1 knockdown by small interfering RNA treatment was found to downregulate neuronal differentiation and to upregulate APP intracellular domain production from APP in fetal neural stem cells. Furthermore, the change in gene expression profiles was systemically examined by DNA microarray. The expression of several genes including ephrin A2 was upregulated by APP-BP1 knockdown as assessed with DNA microarray and reverse transcriptase-polymerase chain reaction. Taken together, our results suggest that APP-BP1 modulates neuronal differentiation by altering gene expression profiles in fetal neural stem cells. © 2012 Lippincott Williams & Wilkins, Inc.