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Ca2+ imaging of identifiable neurons labeled by electroporation in insect brains

Fujiwara, Terufumia; Kazawa, Tomokib; Haupt, Stephan Shuichib; Kanzaki, Ryoheia b

doi: 10.1097/WNR.0b013e32832e7d93
CHEMICAL, SENSES
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SDC

Identifiable neurons in the silkmoth brain were studied physiologically after loading of Ca2+ indicator by local electroporation. Small groups of neurons with projections in confined regions of the antennal lobe were labeled, and Ca2+ imaging showed differences in the dose–response characteristics between projection neurons of the macroglomerular complex that responded to different pheromone components. Compared with projection neurons, local interneurons showed shorter response latencies. Targeted labeling of neurons innervating restricted regions of neuropil by local electroporation is a powerful method that will be generally useful for elucidating details of the functional circuitry in insect brains.

aGraduate School of Information Science and Technology

bResearch Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan

Correspondence to Dr Ryohei Kanzaki, PhD, Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo 153-8904, Japan

Tel/fax: +81 3 5452 5195; e-mail: kanzaki@rcast.u-tokyo.ac.jp

This study was carried out at the Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan

Received 17 April 2009 accepted 5 May 2009

© 2009 Lippincott Williams & Wilkins, Inc.