AUDITORY AND VESTIBULAR SYSTEMSHydrogen protects auditory hair cells from free radicalsKikkawa, Yayoi S.; Nakagawa, Takayuki; Horie, Rie T.; Ito, JuichiAuthor Information Department of Otolaryngology–Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan Correspondence to Dr Takayuki Nakagawa, MD, PhD, Department of Otolaryngology–Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kawahara-cho 54, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan Tel: +81 75 751 3346; fax: +81 75 751 7225; e-mail: email@example.com Received 21 January 2009 accepted 11 February 2009 NeuroReport: May 6th, 2009 - Volume 20 - Issue 7 - p 689-694 doi: 10.1097/WNR.0b013e32832a5c68 Buy SDC Metrics Abstract Reactive oxygen species (ROS) play a role in the degeneration of auditory hair cells because of aging, noise trauma, or ototoxic drugs. Hydrogenation is a fundamental reduction/deoxidation reaction in living organisms. This study thus examined the potential of hydrogen to protect auditory hair cells from ROS-induced damage. To generate ROS, we applied antimycin A to explant cultures of auditory epithelia, and examined the effect of hydrogen on the protection of hair cells against ROS. Incubation with a hydrogen-saturated medium significantly reduced ROS generation and subsequent lipid peroxidation in the auditory epithelia, leading to increased survival of the hair cells. These findings show the potential of hydrogen to protect auditory hair cells from ROS-induced damage. © 2009 Lippincott Williams & Wilkins, Inc.