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In-vivo gene transfer into newly hatched chick brain by electroporation

Yamaguchi, Shinjia; Katagiri, Sachikoa; Hirose, Naokia; Fujimoto, Yasuyukia; Mori, Masahiroa; Fujii-Taira, Ikukob; Takano, Tatsuyaa; Matsushima, Toshiyac; Homma, Koichi J.a

doi: 10.1097/WNR.0b013e3280bef990
BEHAVIOUR

Newly hatched domestic chicks serve as ideal models for studies of the neural basis of behavioral plasticity, particularly for understanding the mechanisms of learning such as filial imprinting. To elucidate the molecular basis and gene functions involved in learning, we developed an in-vivo gene-transfer system in the brain of a living chick using electroporation. When green fluorescent protein-encoding plasmids were transfected to a chick brain, green fluorescence was clearly observed, and expression at the protein level was confirmed by immunoblotting. Most of the transfected brain cells were neuronal cells with dendrites. This neuron-selective electroporation system will facilitate the analysis of gene functions in the living chick brain and provide further clues as to the molecular mechanisms of avian learning.

aFaculty of Pharmaceutical Sciences, Teikyo University, Sagamihara-shi, Kanagawa

bFutai Special Laboratory, Microbial Chemistry Research Center, Microbial Chemistry Research Foundation, CREST, Japan Science and Technology Agency, Shinagawa-ku, Tokyo

cDepartment of Biology, Faculty of Science, Hokkaido University, Hokkaido, Japan

Correspondence to Dr Koichi J. Homma, PhD, Faculty of Pharmaceutical Sciences, Teikyo University, Sagamihara-shi, Kanagawa 229-0195, Japan

Tel: +81 42 685 3739; fax: +81 42 685 3738; e-mail: homma-kj@umin.ac.jp,hommakj@pharm.teikyo-u.ac.jp

Received 15 December 2006; accepted 10 January 2007

© 2007 Lippincott Williams & Wilkins, Inc.