GLIAL CELLSGalectin-3 inhibits Schwann cell proliferation in cultured sciatic nerveGustavsson, Pera; Linsmeier, Cecilia Erikssona; Leffler, Hakonb; Kanje, MartinaAuthor Information Departments of aCell and Organism Biology bLaboratory Medicine, Division of Microbiology, Immunology and Glycobiology-MIG, Lund University, Lund, Sweden Correspondence to Per Gustavsson, Department of Cell and Organism Biology, Lund University, Helgonavägen 3B, SE-223 62, Lund, Sweden Tel: +46 46 222 93 54; fax: +46 46 222 45 39; e-mail: [email protected] Received 15 December 2006; accepted 7 January 2007 NeuroReport: May 7, 2007 - Volume 18 - Issue 7 - p 669-673 doi: 10.1097/WNR.0b013e3280bef97b Buy Metrics Abstract The production of galectin-3, a carbohydrate-binding mammalian lectin, is upregulated in Schwann cells after peripheral nerve injury in areas where Schwann cells proliferate. Here we tested if galectin-3 affected proliferation of Schwann cells in cultured sciatic nerve segments. Galectin-3 significantly decreased the number of bromodeoxyuridine-labelled Schwann cell nuclei. Neither lactose nor a synthetic inhibitor directed against the carbohydrate-binding region abolished the effects of galectin-3. In addition, a mutant galectin-3 unable to bind endogenous carbohydrates had similar effects as normal galectin-3. We conclude that galectin-3 reduces proliferation of Schwann cells in cultured sciatic nerve segments by a mechanism which is independent of its carbohydrate-binding moiety. © 2007 Lippincott Williams & Wilkins, Inc.