Molecular Neurosciences

Localization of mGluR4 protein in the rat cerebral cortex and hippocampus

Phillips, Timothy1,4; Makoff, Andrew2; Brown, Susan3; Rees, Stephen3; Emson, Piers1

Author Information

1Laboratory of Molecular Neuroscience, Department of Neurobiology, Babraham Institute, Cambridge CB2 4AT, UK

2Department of Psychological Medicine, Institute of Psychiatry, Denmark Hill, London SE5 8AF, UK

3Receptor Systems Unit, Glaxo Wellcome Research and Development, Gunnels Wood Rd, Stevenage, Hertfordshire SG1 2NY, UK

4Corresponding Author: Timothy Phillips

ACKNOWLEDGEMENTS: This work was supported by an MRC/Glaxo Wellcome studentship awarded to the author. We would like to thank F. Montague for synthesizing/coupling the peptide and N. Davies for immunizing rabbits and antisera collection. The author is grateful to Dr M. Corsi and Dr F. Ferraguti for their constructive comments. We thank Dr A. Rhodes and A. Barnes for mGluR cDNA.

Website publication 3 October 1997

Received 6 August 1997; accepted 17 August 1997

NeuroReport 8(15):p 3349-3354, October 20, 1997.

Abstract

THE cellular distribution of the rat metabotropic glutamate receptor type 4 (mGluR4) was examined in the adult rat cerebral cortex and hippocampus. Antibodies were raised against amino acid residues located in the extracellular amino terminal domain that is common to both the mGluR4a and mGluR4b splice variants, and used for an immunohistochemical investigation. The affinity-purified antibodies on immunoblot analysis specifically detected mGluR4 protein in transfected mammalian cells, showing no cross-reactivity with other members of the mGluR family. At the light microscope level intense mGluR4-like immunoreactivity was detected in the CA2 region of Ammon's horn in the hippocampus. In the cerebral cortex numerous nonpyramidal cells were strongly immunolabelled.

© Lippincott-Raven Publishers.

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