Molecular Neuroscience: PDF Only

Localization of 5-HT3 receptors in the rat spinal cord

immunohistochemistry and in situ hybridization

Kia, Hossein K.; Miquel, Marie-Christine; McKernan, Ruth M.1; Laporte, Anne-Marie2; Lombard, Marie-Christine3; Bourgoin, Sylvie2; Hamon, Michel2; Vergé, Daniel

Author Information

Département de Neurobiologie des Signaux Intercellulaires, Institut des Neurosciences, CNRS URA 1488, Université Pierre et Marie Curie, 7 Quai Saint-Bernard, 75005 Paris, France; 1Merck Sharp and Dohme Research Laboratories, Harlow, UK; 2Laboratoire de Neurobiologie Cellulaire et Fonctionnelle, INSERM U288, Paris; 3Laboratoire de Neurophysiologie Pharmacologique, INSERM U161, Paris, France

NeuroReport 6(2):p 257-261, January 1995.

Abstract

SPECIFIC antibodies raised against a fusion protein containing the amino acid sequence of the putative second intracellular loop of the cloned 5-HT,-A receptor subunit were used for the immunohistochemical visualization of 5-HT, receptors in the rat spinal cord. A dense 5-HT,-like immunoreactivity was found in the superficial layers of the dorsal horn, which closely matched the labelling of 5-HT, binding sites by [125I]iodo-zacopride. This immuno-staining was markedly decreased following unilateral rhizotomy, consistently with a preferential location of 5-HT, receptors on terminals of primary afferent fibres, and with the presence of 5-HT, mRNA in dorsal root ganglia. However, a significant proportion of 5-HT, receptors persisted after rhizotomy, and the corresponding mRNA was found in the dorsal horn of the spinal cord. 5-HT, receptors are therefore also located on intrinsic neurones of the spinal cord.

© Lippincott-Raven Publishers.

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