ArticlesDifferential subcellular localization of phosphorylated neurofilament and tau proteins in degenerating neurons of the human entorhinal cortexPorchet, Raymonde1; Probst, Alphonse2; Dráberová, Eda3; Dráber, Pavel3; Riederer, Irène M.1,4; Riederer, Beat M.1,4,CAAuthor Information 1Institut de Biologie Cellulaire et de Morphologie, Rue du Bugnon 9, 1005 Lausanne; 2Institute of Pathology, Basel, Switzerland; 3Institute of Molecular Genetics, Academy of Sciences, 14220 Prague, Czech Republic; 4Centre de Neurosciences Psychiatriques, Psychiatric Hospital CERY, 1008 Prilly, Switzerland CACorresponding Author and Address: [email protected] Received 6 March 2003; accepted 17 March 2003 NeuroReport: May 23rd, 2003 - Volume 14 - Issue 7 - p 929-933 doi: 10.1097/01.wnr.0000072844.93264.31 Buy Metrics Abstract A panel of novel monoclonal antibodies was tested on the human entorhinal cortex for the recognition of age- and disease-related changes of neurofilament proteins (NF). Several antibodies identified phosphorylated NF-H subunit, which occurred preferentially in those aged between 60 and 80 years and were localized in degenerating neurons. Such neurons also contained neurofibrillary tangles, but neurofilament aggregates did not co-localize with tangles, nor did the quantity nor the number of NF-positive neurons correlate with the severity of Alzheimer's disease. This points to a susceptibility of NF in a subset of neurons for phosphor-ylation- and metabolically related morphological changes during neurodegeneration. © 2003 Lippincott Williams & Wilkins, Inc.