Cellular, Molecular and Developmental NeuroscienceLong noncoding RNA differentiation antagonizing nonprotein coding RNA promotes the proliferation, invasion and migration of neuroblastoma cells via targeting β-1, 4-galactosyltransferase III by sponging miR-338-3pBi, Chunhuaa,,*; Shan, Jilib,,*; Li, Maoxiangc; Zhang, Qianc; Li, Caihuad; Tong, Jianninge; Huang, QikunfAuthor Information aDepartment of Infectious Diseases, The Affiliated Hospital of Qingdao University bDepartment of Pediatric, Chengyang People’s Hospital cDepartment of Gynaecology and Obstetrics, Rizhao Maternal and Child Health Care Hospital dDepartment of Medical Laboratory, Traditional Chinese Medicine Hospital of Ju County, Rizhao eDepartment of Infectious Disease and Digestion System, Qingdao Women and Children’s Hospital fDepartment of Pediatric, Qilu Hospital of Shandong University (Qingdao), Qingdao, Shandong, China *Dr. Chunhua Bi and Dr. Jili Shan contributed equally to the writing of this article. Received 26 October 2020 Accepted 25 January 2021 Correspondence to Jianning Tong, MB, Department of Infectious Disease and Digestion System, Qingdao Women and Children’s Hospital, Qingdao 266000, Shandong, China, Tel: +86 0532 68661155; e-mail: [email protected] NeuroReport: August 11, 2021 - Volume 32 - Issue 12 - p 965-974 doi: 10.1097/WNR.0000000000001664 Buy Metrics Abstract Neuroblastoma is a common malignant tumor in children, and patients often have a poor prognosis. Long noncoding RNAs (lncRNAs) are involved in the regulation of neuroblastoma progression. However, the regulatory effect of lncRNA differentiation antagonizing nonprotein coding RNA (DANCR) on neuroblastoma is still not clear. The expression levels of DANCR, miR-338-3p and β-1, 4-galactosyltransferase III (B4GALT3) were determined by quantitative real-time PCR. 3-(4, 5-dimethyl-2 thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide, flow cytometry and transwell assays were used to evaluate the proliferation, apoptosis, migration and invasion abilities of neuroblastoma cells. Moreover, western blot analysis was performed to assess the levels of B4GALT3 and the proliferation, apoptosis and migration-related proteins. Besides, a dual-luciferase reporter assay was used to verify the interactions among DANCR, miR-338-3p and B4GALT3. Mice xenograft models were used to ascertain the effect of DANCR on neuroblastoma tumor growth in vivo. Our results revealed that DANCR was highly expressed in neuroblastoma tissues and cells, and its silencing impeded the progression of neuroblastoma cells. DANCR could interact with miR-338-3p. Knockdown of miR-338-3p recovered the inhibitory effect of DANCR knockdown on neuroblastoma progression. B4GALT3 was a target of miR-338-3p. B4GALT3 overexpression reversed the suppression effect of DANCR silencing on neuroblastoma progression. In-vivo experiments further confirmed that DANCR silencing inhibited neuroblastoma tumor growth. Our results indicated that DANCR promoted B4GALT3 expression to increase the proliferation, migration and invasion of neuroblastoma cells via sponging miR-338-3p, which provided a theoretical basis for the targeted therapy of neuroblastoma. Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.