Degeneration and RepairAngiopoietin-1 protects neurons by inhibiting autophagy after neuronal oxygen-glucose deprivation/recovery injuryYin, Zhaoyanga,,*; Gong, Geb,,*; Zhu, Chaoc; Wang, Binc; Sun, Chaoc; Liu, Xinhuic,,#; Yin, Jianc,,#Author Information aDepartment of Orthopedics, the Affiliated Lianyungang Hospital of Xuzhou Medical University, the First People’s Hospital of Lianyungang, Lianyungang bDepartment of Geriatrics, Jinling Hospital, Medical School of Nanjing University cDepartment of Orthopedics, the Affiliated Jiangning Hospital with Nanjing Medical University, Nanjing, PR China *Dr. Zhaoyang Yin and Dr. Ge Gong contributed equally to the writing of this article. #Jian Yin and Xinhui Liu are the co-corresponding author. Received 16 April 2020 Accepted 10 May 2020 Correspondence to Jian Yin, MD, Nanjing Medical University, Nanjing 211100, Jiangsu, PR China, Tel: +8615195969587; fax: +025 52281256; e-mail: firstname.lastname@example.org NeuroReport: August 5, 2020 - Volume 31 - Issue 11 - p 825-832 doi: 10.1097/WNR.0000000000001491 Buy Metrics Abstract Angiopoietin-1 (Ang-1) is a new neuroprotective agent, which can protect neurons from apoptosis. Increased autophagy in neurons subjected to oxygen-glucose deprivation/recovery (OGD/R) injury may lead to autophagic cell death; therefore, the present study investigated the effect of Ang-1 on neurons subjected to OGD/R injury. Neuronal viability was detected by using the Cell Counting Kit-8, which was then used to select the appropriate concentration of Ang-1 and rapamycin used in the OGD/R injury model. The mechanistic role of Ang-1 was observed by detecting the survival rate of neurons and the level of autophagy. Results showed that Ang-1 significantly reduced neuronal cell injury induced by OGD/R and the expression of the autophagy-related proteins LC3 II/I and Beclin-1, and increased the expression of P62/SQSTM1. However, the neuroprotective effects of Ang-1 were counteracted by rapamycin, an autophagy activating agent. The changes of autophagy intensity were further confirmed by transmission electron microscopy observation of autophagosomes. Ang-1 appears to have a neuroprotective role by inhibiting autophagy expression in OGD/R. Thus, these findings could be useful for the treatment of OGD/R injury. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.