Cellular, Molecular and Developmental NeuroscienceThe effect of ascorbic acid pretreatment on amphetamine-induced dopamine depletion in male and female miceGifford, Janace J.; Wagner, George C.Author Information Department of Psychology, Rutgers University, New Jersey, USA Received 17 April 2020 Accepted 23 April 2020 Correspondence to Janace J. Gifford, MS, Department of Psychology, Rutgers University, 152 Frelinghuysen Rd, New Brunswick, NJ 08854, USA, Tel: +(848) 4458937; fax: +(848) 445 2263; e-mail: Janace.email@example.com NeuroReport: July 10, 2020 - Volume 31 - Issue 10 - p 737-740 doi: 10.1097/WNR.0000000000001474 Buy Metrics Abstract The repeated administration of high doses of amphetamine has been shown to cause long-lasting depletions of striatal dopamine which, when substantial enough, have been shown to result in cognitive and motor impairment. These amphetamine-induced lesions are slightly larger in males than that in females and can be partially ameliorated by pretreatment with antioxidants. The objective of the present study was to replicate these two latter observations using an amphetamine dosing regimen that yields only minor depletions of dopamine. It was found that a low-dose treatment of amphetamine using only two subcutaneous injections caused a 57% depletion of striatal dopamine with males slightly more affected than females. Furthermore, pretreatment with ascorbic acid reduced the magnitude of this dopamine depletion with males exhibiting a slightly enhanced protection as compared to females. Compared to the traditionally used high-dose regimens, these effects were mild but in the same direction. The advantage of this regimen is that it better reflects amphetamine-induced depletions of dopamine in humans. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.