Cellular, Molecular and Developmental NeuroscienceIntravenous administration of human amniotic mesenchymal stem cells improves outcomes in rats with acute traumatic spinal cord injury.Zhou, Hong-Longa,,*; Fang, Huaa,,*; Luo, Hai-Taoa; Ye, Min-Huaa; Yu, Guo-Yongb; Zhang, Yana; Mao, Guo-Huaa; Gao, Zi-Yuna; Cheng, Zu-Juea; Zhu, Xin-GenaAuthor Information aDepartment of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang bDepartment of Neurosurgery, Peking University People’s Hospital, Peking University, Beijing, People’s Republic of China *Dr. Hong-Long Zhou and Dr. Hua Fang contributed equally to the writing of this article. Received 9 February 2020 Accepted 16 April 2020 Correspondence to Xin-Gen Zhu, Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, No.1 Min De Road, Dong hu District, Nanchang 330006, People’s Republic of China, Tel: +008679186311396; fax: +86 00167687922; e-Mail: firstname.lastname@example.org NeuroReport: July 10, 2020 - Volume 31 - Issue 10 - p 730-736 doi: 10.1097/WNR.0000000000001473 Buy Metrics Abstract We previously reported that intraspinal transplantation of human amniotic mesenchymal stem cells (hAMSCs) promotes functional recovery in a rat model of acute traumatic spinal cord injury (SCI). However, whether intravenous transplantation of hAMSCs also has therapeutic benefit remains uncertain. In this study, we assessed whether intravenous transplantation of hAMSCs improves outcomes in rats with acute traumatic SCI. In addition, the potential mechanisms underlying the possible benefits of this therapy were investigated. Adult female Sprague–Dawley rats were subjected to SCI using a weight drop device, and then hAMSCs or PBS were administered after 2 h via the tail vein. Our results indicated that transplanted hAMSCs could migrate to injured spinal cord lesion. Compared with the control group, hAMSCs transplantation significantly decreased the numbers of ED1+ macrophages/microglia and caspase-3+ cells, and reduced levels of inflammatory cytokines, such as tumor necrosis factor alpha, interleukin-6 and IL-1β. In addition, hAMSCs transplantation significantly attenuated Evans blue extravasation, promoted angiogenesis and axonal regeneration. hAMSCs transplantation also significantly improved functional recovery. These results suggest that intravenous administration of hAMSCs provides neuroprotective effects in rats after acute SCI, and could be an alternative therapeutic approach for the treatment of acute SCI. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.