Cellular, Molecular and Developmental NeuroscienceEnhanced phosphorylation of S6 protein in mouse cortical layer V and subplate neurons.Lee, Seong-Euna; Hwang, Seojina; Ahn, Sang-Gunb; D’Arcangelo, Gabriellac; Lee, Gum HwaaAuthor Information aCollege of Pharmacy bDepartment of Pathology, College of Dentistry, Chosun University, Gwangju, South Korea cDepartment of Cell Biology and Neuroscience, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA Received 9 March 2020 Accepted 2 April 2020 Supplemental Digital Content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's website, www.neuroreport.com. Correspondence to Gum Hwa Lee, College of Pharmacy, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju 61452, South Korea, Tel: +82 62 230 6371; fax: +82 62 222 5414; e-mail: email@example.com NeuroReport: July 10, 2020 - Volume 31 - Issue 10 - p 762-769 doi: 10.1097/WNR.0000000000001479 Buy SDC Metrics Abstract The mammalian neocortex is composed of six major layers of neurons. Each group of neurons in the cortical layers has distinct characteristics based on the expression of specific genes and connectivity patterns of neural circuits. Neuronal subtype transition and regional identity acquisition are established by temporal cues and interaction between several transcription factors during neurogenesis. The impairment of cortical lamination or neural circuits results in a wide range of neurodevelopmental disorders such as autism, schizophrenia, and certain forms of childhood epilepsy. Despite continuous efforts to classify neurons with the aid of genetic and epigenetic analyses, the neuron-specific properties associated with post-transcriptional modification remain unclear. In the present study, the distribution of phosphorylated S6-positive layers across the neocortex was examined using several layer markers. The development of pS6 S235/236 layers in layer V and the subplate was spatiotemporally regulated in the mouse brain. In addition, enhanced phosphorylation of ribosomal protein S6 in Ctip2-positive layer V neurons in vivo was sustained under in-vitro conditions using a culture of primary cortical neurons. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.