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Concentration- and time-dependent effects of myo-inositol on evoked epileptic afterdischarge in the hippocampus in vivo

Gamkrelidze, Georgi N.a; Nanobashvili, Zaqaria I.b; Bilanishvili, Irina G.b; Lordkipanidze, Tamara,,b; Kandashvili, Mananaa; Kokaia, Merabc; Solomonia, Revaz O.a,,b

doi: 10.1097/WNR.0000000000001341
Degeneration and Repair
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Epilepsy is one of the most widespread neurological diseases characterized by spontaneous recurrent seizures. There is no cure for epilepsy, and available pharmacological treatments with anti-seizure drugs are only symptomatic. Moreover, about third of epilepsy patients are resistant to the anti-seizure drugs. Thus, it is essential to discover new anti-epilepsy drugs. Recently, myo-inositol has been identified as a promising antiepileptic compound. In the present study, using electrophysiological method, we examined for the first time, the effect of myo-inositol on the generation of epileptic afterdischarges in the hippocampus evoked by a local electrical stimulation. This was achieved by implanting two electrodes with a cannula into the same dorsal hippocampus, which allowed for simultaneous local injection of myo-inositol or saline and afterdischarges induction and recording from the same hippocampus. We found that myo-inositol has time- and concentration-dependent effects on the evoked afterdischarges. Specifically, 5 minutes after 1 M myo-inositol infusion, the afterdischarges duration was significantly decreased as compared to preinjection durations in the same animals and also as compared to preinjection level durations in saline injected or contralateral hippocampus myo-inositol infused animals. Further, 0.055 M myo-inositol significantly decreased afterdischarges duration at 5 minutes as compared to 40 minutes post-injection. At both concentrations, the afterdischarges duration recovered to the pre-injection value at 40 minutes after the myo-inositol injection. The present data, taken together with our previous results, strongly suggest that myo-inositol has significant local seizure-suppressant effect.

aInstitute of Chemical Biology, Ilia State University

bI. Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia

cDepartment of Clinical Sciences, Epilepsy Centre, Lund University Hospital, Lund, Sweden

Received 24 July 2019 Accepted 21 August 2019

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Correspondence to Revaz O. Solomonia, PhD, Institute of Chemical Biology, Ilia State University, I. Beritashvili Center of Experimental Biomedicine, K. Cholokashvili av3/5, Tbilisi 0162, Georgia, Tel: +995322220009 x158; fax: +995322231026; e-mail: revaz_solomonia@iliauni.edu.ge

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