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Auxin-mediated rapid degradation of target proteins in hippocampal neurons

Nakano, Risakoa; Ihara, Naokia; Morikawa, Shotaa; Nakashima, Aia; Kanemaki, Masato T.c,,d; Ikegaya, Yujia,,e; Takeuchi, Harukia,,b

doi: 10.1097/WNR.0000000000001299
Cellular, Molecular and Developmental Neuroscience
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Genetic manipulation of protein levels is a promising approach to identify the function of a specific protein in living organisms. Previous studies demonstrated that the auxin-inducible degron strategy provides rapid and reversible degradation of various proteins in fungi and mammalian mitotic cells. In this study, we employed this technology to postmitotic neurons to address whether the auxin-inducible degron system could be applied to the nervous system. Using adeno-associated viruses, we simultaneously introduced enhanced green fluorescent protein (EGFP) fused with an auxin-inducible degron tag and an F-box family protein, TIR1 from Oryza sativa (OsTIR1), into hippocampal neurons from mice. In dissociated hippocampal neurons, EGFP enhanced green fluorescent protein fluorescence signals rapidly decreased when adding a plant hormone, auxin. Furthermore, auxin-induced enhanced green fluorescent protein degradation was also observed in hippocampal acute slices. Taken together, these results open the door for neuroscientists to manipulate protein expression levels by the auxin-inducible degron system in a temporally controlled manner.

aLaboratory of Chemical Pharmacology

bSocial Cooperation Program of evolutional chemical safety assessment system, LECSAS, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan

cMolecular Cell Engineering Laboratory, National Institute of Genetics, Research Organization of Information and Systems (ROIS)

dDepartment of Genetics, The Graduate University for Advanced Studies (SOKENDAI), Mishima, Shizuoka, Japan

eCenter for Information and Neural Networks, National Institute of Information and Communications Technology, Suita, Osaka, Japan

Received 21 May 2019 Accepted 18 June 2019

Correspondence to Haruki Takeuchi, PhD, Social Cooperation Program of evolutional chemical safety assessment system, LECSAS, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan, Tel: +81 3 5841 4784; fax: +81 3 5841 4786; e-mail: haruki-t@mol.f.u-tokyo.ac.jp

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