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Suppressed neurogenesis without cognitive deficits

effects of fast neutron irradiation in mice

Mineyeva, Olga A.a,b; Barykina, Natalia V.a,b; Bezriadnov, Dmitry V.a,b; Latushkin, Sergey T.c; Ryazanov, Alexander I.c; Unezhev, Vitaliy N.c; Shuvaev, Sergey A.b; Usova, Svetlana V.a,b,d; Lazutkin, Alexander A.a,b,e

doi: 10.1097/WNR.0000000000001237

This study assessed the effects of combined low-dose neutron and γ-ray irradiation on hippocampal neurogenesis and hippocampal-dependent memory. Neural progenitor cell division and survival were evaluated in brain sections and whole hippocampal preparations following head irradiation at a dose of 0.34 Gy for neutron radiation and 0.36 Gy for γ-ray radiation. Hippocampal-dependent memory formation was tested in a contextual fear conditioning task following irradiation at doses of 0.4 Gy for neutron radiation and 0.42 Gy for γ-ray radiation. Cell division was suppressed consistently along the entire dorsoventral axis of the hippocampus 24 h after the irradiation, but quiescent stem cells remained unaffected. The control and irradiated mice showed no differences in terms of exploratory behavior or anxiety 6 weeks after the irradiation. The ability to form hippocampus-dependent memory was also unaffected. The data may be indicative of a negligible effect of the low-dose of fast neutron irradiation and the neurogenesis suppression on animal behavior at 6 weeks after irradiation.

aLaboratory of Neurobiology of Memory, P.K. Anokhin Research Institute of Normal Physiology

bBrain Stem Cell Laboratory, Moscow Institute of Physics and Technology

cNational Research Center ‘Kurchatov Institute’

dLaboratory of Human Cell Neurophysiology, Semenov Institute of Chemical Physics

eN.N. Burdenko National Scientific and Practical Center for Neurosurgery, Moscow, Russia

Correspondence to Alexander A. Lazutkin, PhD, P.K. Anokhin Research Institute of Normal Physiology, 8 Baltiyskaya Street, 125315 Moscow, Russia Tel/fax: +7 495 601 2245; e-mail:

Received March 7, 2019

Accepted March 9, 2019

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