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Immature electrophysiological properties of human-induced pluripotent stem cell-derived neurons transplanted into the mouse cortex for 7 weeks

Kayama, Tasukua; Okamoto, Kazukia; Gao, Mengxuana; Ikegaya, Yujia,b; Sasaki, Takuyaa,c

doi: 10.1097/WNR.0000000000001178

The transplantation of human-induced pluripotent stem cell (hiPSC)-derived cells has emerged as a potential clinical approach for the treatment of brain diseases. Recent studies with animal disease models have shown that hiPSC-derived neurons transplanted into the brain, especially the nigrostriatal area, could restore degenerated brain functions. Further works are required to test whether hiPSC-derived neurons can also gain functional properties for other cortical areas. In this study, hiPSC-derived neurospheres were transplanted into the adult mouse hippocampus and sensory cortex. Most transplanted hiPSC-derived neurons expressed both Nestin and NeuN at 7 weeks after transplantation. Whole-cell patch-clamp recordings from brain slices indicated that transplanted cells showed no action potentials upon current injection and few small inward currents, indicating that hiPSC-derived neurons did not become functionally mature within these time periods.

aGraduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo

bCenter for Information and Neural Networks, Suita City

cPrecursory Research for Embryonic Science and Technology), Japan Science and Technology Agency, Kawaguchi, Japan

Correspondence to Takuya Sasaki, PhD, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo 113-0033, Japan Tel: +81 358 414 783; fax: +81 358 414 786; e-mail:

Received October 23, 2018

Accepted November 19, 2018

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