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Effects of ginkgo biloba extract on the cognitive function and expression profile of inflammatory factors in a rat model of hemorrhagic stroke

Luo, Chen; Fan, Li-Hong; Zhang, Hua; Zhao, Jing; Li, Lei; Zhang, Lian; Zhang, Hai-Xiao; Ma, Ming-Ming

doi: 10.1097/WNR.0000000000001072

Hemorrhagic stroke is a major risk factor for cognitive impairment. Our study aimed to measure the effect of ginkgo biloba extract (EGB761) on the cognitive ability and inflammatory expression in hemorrhagic stroke model SD rats and to analyze their relationship. Forty SD rats were divided randomly into an SD group (normal control SD rats), an SD+EGB761 group (normal control SD rats supplemented with 45 mg/kg EGB761), a CO group (hemorrhagic stroke model SD rats using collagenase), and a CO+EGB761 group (hemorrhagic stroke model SD rats supplemented with 45 mg/kg EGB761) consisting of 10 rats, respectively. The Y-electric maze test was selected to measure the cognitive function in four groups. Furthermore, enzyme-linked immunosorbent assay and real-time PCR were, respectively, applied for detecting the protein and gene expression profiles of inflammatory factors in primary cultured microglia. Compared with rats in the SD group, the average time of electrical simulation for mastering criteria was prolonged in the CO group (P<0.05). Furthermore, expression levels of proinflammatory cytokines interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α and anti-inflammatory cytokines IL-4, IL-10, and tumor necrosis factor-β were significantly increased and decreased, respectively, in rats of the CO group compared with the SD group (P<0.05). The results of electrical simulation time, inflammatory factors protein, and gene expression profile in rats of the CO+EGB761 group compared with the CO group were opposite to above contrast (P<0.05). Ginkgo biloba extract could alleviate the cognitive dysfunction after hemorrhagic stroke in SD rats; this is associated with regulating the expression of inflammatory factors secreted by microglia.

Department of Neurology, Hangzhou Red Cross Hospital, Hangzhou, Zhejiang Province, China

Correspondence to Chen Luo, MD, Department of Neurology, Hangzhou Red Cross Hospital, #208 East Huancheng Road, Shangcheng District, Hangzhou 310009, China Tel/fax: +86 571 5610 9782; e-mail:

Received March 21, 2018

Accepted May 23, 2018

© 2018 Wolters Kluwer Health | Lippincott Williams & Wilkins