Institutional members access full text with Ovid®

Share this article on:

SIRT2 and Akt mediate NAD+-induced and NADH-induced increases in the intracellular ATP levels of BV2 microglia under basal conditions

Zhang, Jie; Wang, Caixia; Ying, Weihai

doi: 10.1097/WNR.0000000000000876
Cellular, Molecular and Developmental Neuroscience

NAD+ replenishment can restore ATP levels and rescue premature aging in Cockayne syndrome mice. However, there has been no mechanistic study regarding the effects of NAD+ and NADH on intracellular ATP levels under basal conditions. In our current study, we used BV2 microglia to test our hypothesis that NAD+ and NADH can increase intracellular ATP levels under basal conditions. We found that both NAD+ and NADH significantly increased the intracellular ATP levels of BV2 microglia, which were attenuated by SIRT2 siRNA, the SIRT2 inhibitor AGK2, and the phosphatidylinositol 3-kinase/Akt inhibitor LY294002. Our study has also suggested that SIRT2 mediates the NAD+-induced and NADH-induced increase in Akt phosphorylation in BV2 microglia. Collectively, our study has suggested that SIRT2 mediates both NAD+-induced and NADH-induced increases in the intracellular ATP levels of BV2 microglia by modulating Akt phosphorylation.

School of Biomedical Engineering, Med-X Research Institute, Division of Disease Studies, Shanghai Jiao Tong University, Shanghai, People’s Republic of China

Correspondence to Weihai Ying, PhD, School of Biomedical Engineering, Med-X Research Institute, Division of Disease Studies, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, People’s Republic of China Tel: +86 216 293 3075; fax: +86 216 293 2302; e-mail: weihaiy@sjtu.edu.cn

Received May 5, 2017

Accepted July 17, 2017

© 2018 Wolters Kluwer Health | Lippincott Williams & Wilkins