Institutional members access full text with Ovid®

Share this article on:

Prolonged duration of isoflurane anesthesia impairs spatial recognition memory through the activation of JNK1/2 in the hippocampus of mice

Jiang, Shan; Miao, Bei; Chen, Ying

doi: 10.1097/WNR.0000000000000760
Clinical Neuroscience

Postoperative cognitive dysfunction is a frequent complication with surgery and anesthesia, and the underlying mechanism is unclear. Our aim was to investigate the effect of different durations of isoflurane anesthesia on spatial recognition memory and activation of JNK1/2 in the hippocampus of mice. In the present study, adult male mice were anesthetized with isoflurane for different durations (1.5% isoflurane for 1, 2, and 4 h). Spatial recognition memory was determined using spontaneous alternation and two-trial recognition memory in Y-maze at 24 h after anesthesia. The activation of JNK1/2 in the hippocampus was tested using western blot. Mice treated with isoflurane for 4 h showed significantly decreased spontaneous alternations and decreased exploration parameters compared with the no anesthesia group, but this was not observed in mice treated with isoflurane for 1 or 2 h. The protein levels of p-JNK1/2 in the hippocampus were significantly increased at 10 min after isoflurane anesthesia for 1, 2, and 4 h compared with no anesthesia. However, only isoflurane anesthesia for 4 h still increased JNK1/2 and p-JNK1/2 levels at 24 h after anesthesia. We concluded that prolonged duration of isoflurane anesthesia maintained the activation of JNK1/2, which led to memory impairment at 24 h after anesthesia.

aDepartment of Anesthesiology, The First People’s Hospital of Lianyungang

bDepartment of Anatomy and Physiology Lianyungang Branch of Traditional Chinese Medicine, Jiangsu Union Technical Institute, Lianyungang

cDepartment of Gastroenterology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China

* Shan Jiang and Bei Miao contributed equally to the writing of this article.

Correspondence Ying Chen, MD, Department of Anesthesiology, The first People's Hospital of Lianyungang, 182 Tongguan North Road, Lianyungang City 222002, Jiangsu Province, China Tel: +86 0518 85452253; fax: +86 0518 85456700; e-mail:

Received January 12, 2017

Accepted February 8, 2017

© 2017 Wolters Kluwer Health | Lippincott Williams & Wilkins