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MiR-200a impairs glioma cell growth, migration, and invasion by targeting SIM2-s

Su, Yuhanga,b,c; He, Qiaoweid; Deng, Linb,c; Wang, Juntaob,c; Liu, Qinglinb,c; Wang, Donghaib,c; Huang, Qibinga,b,c; Li, Gangb,c

doi: 10.1097/WNR.0000000000000032
CELLULAR, MOLECULAR AND DEVELOPMENTAL NEUROSCIENCE
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Recently, single-minded homolog 2-short form (SIM2-s) was reported to be related to tumor development and progression and to be elevated in many human cancer cells. In this study, we investigated the factors that contribute to the regulation of SIM2-s expression in gliomas. The results showed that SIM2-s was elevated in gliomas. In addition, inhibition of SIM2-s reduced glioma cell growth, migration, and invasion. Next, we demonstrated that SIM2-s is a functional target of miR-200a. Further, miR-200a is downregulated in human glioma and inhibition of miR-200a caused upregulation of SIM2-s in T98G cells and promoted their motility. Finally, blockage of miR-200a expression in a mouse model of human glioma resulted in significant promotion of tumor growth. These findings suggest that miR-200a could serve as a therapeutic tool for glioma.

Departments of aEmergency Surgery

bNeurosurgery, Qilu Hospital of Shandong University

cBrain Science Research Institute, Shandong University, Jinan

dDepartment of Neurology, The Affiliated Yantai Yuhuangding Hospital of Qingdao Medical College, Yantai, China

Correspondence to Gang Li, Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012, China Tel: +86 0531 82166615; fax: +86 0531 82166615; e-mail: doctorligang@126.com

Received July 29, 2013

Accepted August 20, 2013

© 2014 Wolters Kluwer Health | Lippincott Williams & Wilkins