CELLULAR, MOLECULAR AND DEVELOPMENTAL NEUROSCIENCEMetformin inhibits glioma cell U251 invasion by downregulation of fibulin-3Gao, Lian-Bo; Tian, Shen; Gao, Hong-Hua; Xu, Yan-Yuan Author Information Department of Neurology, The 4th Affiliated Hospital of China Medical University, Shenyang, China Correspondence to Lian-Bo Gao, Department of Neurology, The 4th Affiliated Hospital of China Medical University, Shenyang 110032, China Tel/fax: +86 024 625 71500; e-mail: [email protected] Received November 4, 2012 Accepted December 28, 2012 NeuroReport: July 10, 2013 - Volume 24 - Issue 10 - p 504-508 doi: 10.1097/WNR.0b013e32836277fb Buy Metrics Abstract Fibulin-3 has been considered as a regulator of glioma cell invasion, but little is known about the molecules regulating fibulin-3 expression. Metformin, an oral antidiabetic drug in the biguanide class, is known to inhibit proliferation and metastasis in a variety of cancer cells. In the present study, we determined the effect of metformin on the expression of fibulin-3 in U251 Human glioma cells. Metformin potently suppressed U251 cell adhesion and invasion. Metformin inhibited the expression of fibulin-3 at the transcriptional level. Moreover, metformin abolished the protein expression of fibulin-3 in a concentration-dependent manner. Furthermore, this compound suppressed the expression of matrix metalloproteinase-2, a key effector of glioma cell invasion, regulated by fibulin-3. Taken together, our results suggest that metformin abolishes fibulin-3 expression and subsequently inhibits invasion of glioma cells. © 2013 Lippincott Williams & Wilkins, Inc.