DEGENERATION AND REPAIRNeonatal SVZ EGFP-labeled cells produce neurons in the olfactory bulb and astrocytes in the cerebral cortex by in-vivo electroporationWang, Xijuana; Chang, Liwena; Guo, Zhibaob; Li, Wenbina; Liu, Weia; Cai, Baohuana; Wang, JingaAuthor Information aDepartment of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei bDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China Correspondence to Liwen Chang, BSc, Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jie Fang Avenu, Wuhan, Hubei, China Tel: +86-27-83663458; fax: +86-27-83646605; e-mail: [email protected] Received December 23, 2012 Accepted March 5, 2013 NeuroReport: May 8, 2013 - Volume 24 - Issue 7 - p 381-387 doi: 10.1097/WNR.0b013e328360f7ef Buy Metrics Abstract Neural progenitors/stem cells (NSCs) exist in neonatal mouse subventricular zone (SVZ). To explore the differentiation of the NSCs in neonatal mouse SVZ and the distribution of the progeny cells derived from these NSCs in early adulthood, the enhanced green fluorescent protein (EGFP) plasmid was transferred into the NSCs in the lateral ventricle of newborn mice (P0) by in-vivo electroporation to trace these cells and their progeny cells. Thirty days after electroporation, histological sections of mouse brain were prepared for immunofluorescence with cell-specific antibodies to identify the type(s) of cells that were marked by EGFP. The results showed that EGFP-positive cells were distributed mainly in the olfactory bulb (OB), cortex, and SVZ, and double labeled with NeuN (neuron marker) in OB, glial fibrillary acidic protein (GFAP) (astrocyte marker) in the cortex, and Blbp and GFAP (astrocyte marker) in SVZ. However, there was no-EGFP-positive cell in the hippocampus. The present results indicate that the NSCs in SVZ of the neonatal mouse can give rise to neurons in the OB and astrocytes in the cortex in early adulthood, but not generate progeny cells residing in the hippocampus. In addition, there are still neural progenitors in SVZ until early adulthood. © 2013 Lippincott Williams & Wilkins, Inc.