CELLULAR, MOLECULAR AND DEVELOPMENTAL NEUROSCIENCEEffects of controlled release of brain-derived neurotrophic factor from collagen gel on rat neural stem cellsHuang, Feia; Yin, Zongshengb; Wu, Danc; Hao, JiqingdAuthor Information aDepartment of Orthopaedics, The Fourth Affiliated Hospital of Anhui Medical University Departments of bOrthopaedics cScience-education dOncology, The First Affiliated Hospital of Anhui Medical University, Hefei, People’s Republic of China Correspondence to Jiqing Hao, MD, Department of Oncology, The First Affiliated Hospital of Anhui Medical University, No 218 Jixi Road, Shushan District, Hefei 230000, Anhui Province, People’s Republic of China Tel: +86 551 2922945; fax: +86 551 2922945; e-mail: email@example.com Received October 21, 2012 Accepted November 13, 2012 NeuroReport: February 13th, 2013 - Volume 24 - Issue 3 - p 101-107 doi: 10.1097/WNR.0b013e32835c93c5 Buy SDC Metrics Abstract This study aimed to determine the effects of the controlled release of brain-derived neurotrophic factor (BDNF) from collagen gel on rat neural stem cells (NSCs). With two groups of daily addition of BDNF and collagen gel without BDNF as controls, BDNF was tested using ELISA at different time points. In the BDNF-collagen gel group, BDNF was steadily released from gels for 10 days. The cell viability test and the bromodeoxyuridine incorporation assay showed that the BDNF-collagen gel supported the survival and proliferation of NSCs. Compared with controls, the length of processes was markedly longer and the differentiation percentage from NSCs into neurons was much higher in the BDNF-collagen gel group (P<0.05). These findings suggest that BDNF-collagen gel can significantly reduce the amount of BDNF required for the culture of NSCs and increase the differentiation percentage from NSCs into neurons. © 2013 Lippincott Williams & Wilkins, Inc.