REGENERATION AND TRANSPLANTATIONTransplantation of an adipose stem cell cluster in a spinal cord injuryOh, Jin Sooa,b; Park, In Suc; Kim, Keung Nyuna; Yoon, Do Heuma,b; Kim, Sang-Heonc; Ha, YoonaAuthor Information aDepartment of Neurosurgery, Spine and Spinal Cord Institute bBrain Korea 21 Project for Medical Science, College of Medicine, Yonsei University cBiomaterial Research Center, Division of Life Sciences, Korea Institute of Science and Technology, Seoul, Korea Jin Soo Oh and In-Su Park contributed equally to the writing of this article. Correspondence to Yoon Ha, MD, PhD, Department of Neurosurgery, Spine and Spinal Cord Institute, Yonsei University College of Medicine, 120-752, 134 Shinchon-dong, Seodaemoon-gu, Seoul, Korea Tel: +82 2 2228 2165; fax: +82 2 393 9979; e-mail:[email protected] and Sang-Heon Kim, PhD, Biomaterial Research Center, Division of Life Sciences, Korea Institute of Science and Technology, 39-1 Hawolgok, Seongbuk, Seoul 136-791, Korea Tel: +82 2 958 5344; fax: +82 2 958 5308; e-mail: [email protected] Received November 15, 2011 Accepted December 13, 2011 NeuroReport: March 28, 2012 - Volume 23 - Issue 5 - p 277-282 doi: 10.1097/WNR.0b013e3283505ae2 Buy Metrics Abstract We investigated whether transplantation of a three-dimensional cell mass of adipose-derived stem cells (3DCM-ASCs) improved hind limb functional recovery by the stimulation of angiogenesis and neurogenesis in a spinal cord injury. In in-vitro experiments, we confirmed that 3DCM-ASCs differentiated into CD31-positive endothelial cells. To evaluate the therapeutic effect of 3DCM-ASCs in vivo, PBS, human adipose tissue-derived stem cells, and 3DCM-ASCs were transplanted into a spinal cord injury model. The 3DCM-ASCs transplanted into the injured spinal cord differentiated into CD31-positive endothelial cells and remained differentiated. Transplantation of 3DCM-ASCs into the injured spinal cord significantly elevated the density of vascular formations through angiogenic factors released by the 3DCM-ASCs at the lesion site, and enhanced axonal outgrowth at the lesion site. Consistent with these results, the transplantation of 3DCM-ASCs significantly improved functional recovery compared with both ASC transplantation and PBS treatment. These findings suggest that transplantation of 3DCM-ASCs may be an effective stem cell therapy for the treatment of spinal cord injuries and neural ischemia. © 2012 Lippincott Williams & Wilkins, Inc.