MOLECULAR NEUROSCIENCEc-Jun induction is independent of early growth response factor during cerebellar granule neuron apoptosisSong, Bina,b,c; Xie, Boa,b,c; Wang, Chonga,b,c; Li, Mingtaoa,b,cAuthor Information aDepartment of Pharmacology bThe Proteomics Center cCenter for Neuroscience, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China Bin Song and Bo Xie contributed equally to this study. Correspondence to Dr Mingtao Li, PhD, Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, China Tel: +86 20 87330161; fax: +86 20 87331653; e-mail: [email protected] Received September 15, 2011 Accepted October 23, 2011 NeuroReport: January 25, 2012 - Volume 23 - Issue 2 - p 67-72 doi: 10.1097/WNR.0b013e32834e7d69 Buy Metrics Abstract Early growth response factor 1 (Egr-1) is induced during activity deprivation-dependent apoptosis in cerebellar granule neurons and plays a critical role in neuronal apoptosis. It has been proposed that c-Jun lies downstream of the Egr-dependent proapoptotic signaling cascade in cerebellar granule neurons. However, our data show that neither suppressing Egr activity using a dominant-negative mutant nor specifically silencing Egr-1 using small interfering RNA inhibited the activation and expression of c-Jun after activity deprivation in cerebellar granule neurons. Furthermore, two inhibitors, mithramycin A and chromomycin A3, which repressed transcription by selectively displacing GC-rich DNA-binding transcription factors (including those of the Egr family), did not attenuate the activation and expression of c-Jun during neuronal apoptosis. We conclude that c-Jun induction is independent of Egr during apoptosis induced by activity deprivation in cerebellar granule neurons. © 2012 Lippincott Williams & Wilkins, Inc.