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Comparison of AAV2 and AAV5 in gene transfer in the injured spinal cord of mice

Peng, Su-Pinga; Kügler, Sebastianb; Ma, Zhi-Kuia; Shen, Yan-Qina; Schachner, Melittaa

doi: 10.1097/WNR.0b013e328348bff5
REGENERATION AND TRANSPLANTATION
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Recombinant adeno-associated virus (AAV) vectors are promising tools for gene therapy. In spinal cord injury where extensive damage occurs, vectors with high diffusion and transduction abilities are required. We compared the diffusion capacity and transduction efficiency of AAV2 and AAV5 vectors using a mouse spinal cord injury model. Our study demonstrates that AAV5 is more effective than AAV2 for delivering genes into the injured spinal cord tissue. AAV5 diffused 6.9 mm from the injection site, transduced with an approximately two-fold increase in total cell number and yielded an approximately three-fold increase in gene expression in comparison with AAV2.

aCenter for Neuroscience, Shantou University Medical College, Shantou, Guangdong Province, People's Republic of China

bDeutsche Forschungsgemeinschaft Research Center for Molecular Physiology of the Brain, Department of Neurology, University of Medicine Göttingen, Göttingen, Germany

Correspondence to Yan-Qin Shen, PhD, Center for Neuroscience, Shantou University Medical College, 22 Xin Ling Road, Shantou 515041, People's Republic of China Tel:+86 0754 8890 0276; fax:+86 0754 8890 0236; e-mail: yqshen@stu.edu.cn and Melitta Schachner, PhD, Center for Neuroscience, Shantou University Medical College, 22 Xin Ling Road, Shantou 515041, People's Republic of China Tel: + 86 0754 8890 0276; fax: + 86 0754 8890 0236; e-mail: schachner@stu.edu.cn

Received April 26, 2011

Accepted May 12, 2011

© 2011 Lippincott Williams & Wilkins, Inc.