AUDITORY AND VESTIBULAR SYSTEMSChanges in guinea pig cochlea after transient cochlear ischemiaLin, Chia-Dera b; Wei, I-Huac; Tsai, Mang-Hungc; Kao, Ming-Chinge f; Lai, Chih-Hod; Hsu, Chuan-Jeng; Oshima, Takeshih; Tsai, Ming-Hsuia bAuthor Information aDepartment of Otolaryngology-Head and Neck Surgery, China Medical University Hospital bGraduate Institute of Clinical Medical Science Departments of cAnatomy dMicrobiology, School of Medicine eDepartment of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung fDepartment of Biochemistry, National Defense Medical Center gDepartment of Otolaryngology-Head and Neck Surgery, National Taiwan University Hospital, Taipei, Taiwan hDepartment of Otolaryngology-Head and Neck Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan Correspondence to Ming-Hsui Tsai, MD, Department of Otolaryngology-Head and Neck Surgery, China Medical University Hospital, No. 2, Yude Road, North District, Taichung City, Taiwan Tel/fax: +886 4 22052121 x4436; e-mail: [email protected] Received 30 May 2010 accepted 27 June 2010 NeuroReport: October 27, 2010 - Volume 21 - Issue 15 - p 968-975 doi: 10.1097/WNR.0b013e32833da3c3 Buy Metrics Abstract Perturbation of cochlear microcirculation, that is, ischemia is a major cause of hearing impairment. Earlier studies examined the short-term (≤7 days) effect of cochlear ischemia. This study characterized the long-term (4 weeks) functional and morphological changes in adult guinea pig cochleae subject to transient ischemia by clamping the labyrinthine artery for 0.25–3 h. Notably, cochlear ischemia for over 1 h caused an increase of auditory brainstem response thresholds and loss of high-frequency hearing, basal-turn hair cells, and spiral ganglions. Auditory recovery may be possible after 30-min ischemia. The extent of the functional and morphological changes depended on the ischemia period, and the changes progressed in extent from the apical to the basal turn in an orderly fashion. © 2010 Lippincott Williams & Wilkins, Inc.